Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep
Background: Cystic echinococcosis caused by the metacestode of Echinococcus granulosus is a major problem in both humans and domestic animals health. There-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (CE) is still needed. Met...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-06-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/410 |
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| author | Zahra Jahani Behnam Meshgi Masomeh Rajabi-Bzl Fatemeh Jalousian Sajad Hasheminasab |
| author_facet | Zahra Jahani Behnam Meshgi Masomeh Rajabi-Bzl Fatemeh Jalousian Sajad Hasheminasab |
| author_sort | Zahra Jahani |
| collection | DOAJ |
| description | Background: Cystic echinococcosis caused by the metacestode of Echinococcus granulosus is a major problem in both humans and domestic animals health. There-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (CE) is still needed.
Methods: In the present work, antigen B labeled with gold nanoparticles was used to detect antibodies against hydatid cyst disease. The prepared antigen B was ana-lyzed by SDS-PAGE. Tetra chloroauric acid (HAuCl4) was used to produce colloi-dal gold and antigen B labeled by gold nanoparticles, then it was tested by using rabbits antisera and sera from naturally infected sheep. The labeled antigen B was evaluated using Dot-immunogold staining (Dot-IGS) method.
Results: Electrophoretic pattern of hydatid cyst fluid showed the quality of bands in the condensed fluid is better than crude fluid. SDS-PAGE analysis cyst fluid and antigen B revealed three specific protein bands that were detected at molecular weights of 24, 30 and 40 kDa that all are the subunits of antigen B. Evaluation of antigen B labeled by gold nanoparticles by using Dot-IGS technique showed 1/1 and 1/50 dilutions in comparison with another has the best immunoreaction. In this method, nanoparticles produced a typical purple color, when they binded to the strip at the site of immunoreaction.
Conclusion: Therefore, using gold nanoparticles is a good candidate for detection of helminthiasis, also as selective tools of early detection, simple and cost-effective, regardless of specific skills and equipment with optimal durability. |
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| format | Article |
| id | doaj.art-e6d2a7b81420468cb80e3de99e61ba79 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-24T04:12:29Z |
| publishDate | 2014-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-e6d2a7b81420468cb80e3de99e61ba792022-12-21T17:16:01ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2014-06-0192389Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected SheepZahra Jahani0Behnam Meshgi1Masomeh Rajabi-Bzl2Fatemeh Jalousian3Sajad Hasheminasab4Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Dept. of Medical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Background: Cystic echinococcosis caused by the metacestode of Echinococcus granulosus is a major problem in both humans and domestic animals health. There-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (CE) is still needed. Methods: In the present work, antigen B labeled with gold nanoparticles was used to detect antibodies against hydatid cyst disease. The prepared antigen B was ana-lyzed by SDS-PAGE. Tetra chloroauric acid (HAuCl4) was used to produce colloi-dal gold and antigen B labeled by gold nanoparticles, then it was tested by using rabbits antisera and sera from naturally infected sheep. The labeled antigen B was evaluated using Dot-immunogold staining (Dot-IGS) method. Results: Electrophoretic pattern of hydatid cyst fluid showed the quality of bands in the condensed fluid is better than crude fluid. SDS-PAGE analysis cyst fluid and antigen B revealed three specific protein bands that were detected at molecular weights of 24, 30 and 40 kDa that all are the subunits of antigen B. Evaluation of antigen B labeled by gold nanoparticles by using Dot-IGS technique showed 1/1 and 1/50 dilutions in comparison with another has the best immunoreaction. In this method, nanoparticles produced a typical purple color, when they binded to the strip at the site of immunoreaction. Conclusion: Therefore, using gold nanoparticles is a good candidate for detection of helminthiasis, also as selective tools of early detection, simple and cost-effective, regardless of specific skills and equipment with optimal durability.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/410Antigen BDiagnosisHydatid cystNanoparticle |
| spellingShingle | Zahra Jahani Behnam Meshgi Masomeh Rajabi-Bzl Fatemeh Jalousian Sajad Hasheminasab Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep Iranian Journal of Parasitology Antigen B Diagnosis Hydatid cyst Nanoparticle |
| title | Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep |
| title_full | Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep |
| title_fullStr | Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep |
| title_full_unstemmed | Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep |
| title_short | Improved Serodiagnosis of Hydatid Cyst Disease Using Gold Nanoparticle Labeled Antigen B in Naturally Infected Sheep |
| title_sort | improved serodiagnosis of hydatid cyst disease using gold nanoparticle labeled antigen b in naturally infected sheep |
| topic | Antigen B Diagnosis Hydatid cyst Nanoparticle |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/410 |
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