| Summary: | Products derived from <i>Cannabis sativa</i> L. have gained increased interest and popularity. As these products become common amongst the public, the health and potential therapeutic values associated with hemp have become a premier focus of research. While the psychoactive and medicinal properties of <i>Cannabis</i> products have been extensively highlighted in the literature, the antibacterial properties of cannabidiol (CBD) have not been explored in depth. This research serves to examine the antibacterial potential of CBD against <i>Salmonella newington</i> and <i>S</i>. <i>typhimurium</i>. In this study, we observed bacterial response to CBD exposure through biological assays, bacterial kinetics, and fluorescence microscopy. Additionally, comparative studies between CBD and ampicillin were conducted against <i>S</i>. <i>typhimurium</i> and <i>S</i>. <i>newington</i> to determine comparative efficacy. Furthermore, we observed potential resistance development of our <i>Salmonella</i> spp. against CBD treatment.
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