Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation
The <i>Kluyveromyces marxianus </i>yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandator...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/2/62 |
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| author | Konstantins Dubencovs Janis Liepins Arturs Suleiko Anastasija Suleiko Reinis Vangravs Jana Kassaliete Rita Scerbaka Oskars Grigs |
| author_facet | Konstantins Dubencovs Janis Liepins Arturs Suleiko Anastasija Suleiko Reinis Vangravs Jana Kassaliete Rita Scerbaka Oskars Grigs |
| author_sort | Konstantins Dubencovs |
| collection | DOAJ |
| description | The <i>Kluyveromyces marxianus </i>yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density <em>K. marxianus</em> fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for <em>K. marxianus</em> biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L<sup>-1 </sup>and the 2-PE biosynthesis rate was 0.372 g·L<sup>−1</sup>·h<sup>−1</sup>, with a yield of 74% from 2-phenylalanine. |
| first_indexed | 2024-03-10T12:17:49Z |
| format | Article |
| id | doaj.art-e6d590c7c19945369ea22fcace46d6e5 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T12:17:49Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-e6d590c7c19945369ea22fcace46d6e52023-11-21T15:46:41ZengMDPI AGFermentation2311-56372021-04-01726210.3390/fermentation7020062Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch CultivationKonstantins Dubencovs0Janis Liepins1Arturs Suleiko2Anastasija Suleiko3Reinis Vangravs4Jana Kassaliete5Rita Scerbaka6Oskars Grigs7Laboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaInstitute of Microbiology and Biotechnology, University of Latvia, Jelgavas Street 1, LV-1004 Riga, LatviaLaboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaLaboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaInstitute of Microbiology and Biotechnology, University of Latvia, Jelgavas Street 1, LV-1004 Riga, LatviaInstitute of Microbiology and Biotechnology, University of Latvia, Jelgavas Street 1, LV-1004 Riga, LatviaInstitute of Microbiology and Biotechnology, University of Latvia, Jelgavas Street 1, LV-1004 Riga, LatviaLaboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, LatviaThe <i>Kluyveromyces marxianus </i>yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density <em>K. marxianus</em> fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for <em>K. marxianus</em> biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L<sup>-1 </sup>and the 2-PE biosynthesis rate was 0.372 g·L<sup>−1</sup>·h<sup>−1</sup>, with a yield of 74% from 2-phenylalanine.https://www.mdpi.com/2311-5637/7/2/62<i>Kluyveromyces marxianus</i>2-phenylethanolfed-batchcultivationbioreactormodel predictive control (MPC) |
| spellingShingle | Konstantins Dubencovs Janis Liepins Arturs Suleiko Anastasija Suleiko Reinis Vangravs Jana Kassaliete Rita Scerbaka Oskars Grigs Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation Fermentation <i>Kluyveromyces marxianus</i> 2-phenylethanol fed-batch cultivation bioreactor model predictive control (MPC) |
| title | Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation |
| title_full | Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation |
| title_fullStr | Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation |
| title_full_unstemmed | Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation |
| title_short | Optimization of Synthetic Media Composition for <i>Kluyveromyces marxianus</i> Fed-Batch Cultivation |
| title_sort | optimization of synthetic media composition for i kluyveromyces marxianus i fed batch cultivation |
| topic | <i>Kluyveromyces marxianus</i> 2-phenylethanol fed-batch cultivation bioreactor model predictive control (MPC) |
| url | https://www.mdpi.com/2311-5637/7/2/62 |
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