Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis
Research background. Cellulose is an ingredient of waste materials that can be converted to other valuable substances. This is possible provided that the polymer molecule is degraded to smaller particles and used as a carbon source by microorganisms. Because of the frequently applied methods of pret...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2021-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/384093 |
| _version_ | 1797206897443471360 |
|---|---|
| author | Krzysztof Makowski Martyna Leszczewicz Natalia Broncel Lidia Lipińska-Zubrycka Adrian Głębski Piotr Komorowski Bogdan Walkowiak |
| author_facet | Krzysztof Makowski Martyna Leszczewicz Natalia Broncel Lidia Lipińska-Zubrycka Adrian Głębski Piotr Komorowski Bogdan Walkowiak |
| author_sort | Krzysztof Makowski |
| collection | DOAJ |
| description | Research background. Cellulose is an ingredient of waste materials that can be converted to other valuable substances. This is possible provided that the polymer molecule is degraded to smaller particles and used as a carbon source by microorganisms. Because of the frequently applied methods of pretreatment of lignocellulosic materials, the cellulases derived from thermophilic microorganisms are particularly desirable.
Experimental approach. We were looking for cellulolytic microorganisms able to grow at 50 °C and we described their morphological features and biochemical characteristics based on carboxymethyl cellulase (CMCase) activity and the API® ZYM system. The growth curves during incubation at 50 °C were examined using the BioLector® microbioreactor.
Results and conclusions. Forty bacterial strains were isolated from fermenting hay, geothermal karst spring, hot spring and geothermal pond at 50 °C. The vast majority of the bacteria were Gram-positive and rod-shaped with the maximum growth temperature of at least 50 °C. We also demonstrated a large diversity of biochemical characteristics among the microorganisms. The CMCase activity was confirmed in 27 strains. Hydrolysis capacities were significant in bacterial strains: BBLN1, BSO6, BSO10, BSO13 and BSO14, and reached 2.74, 1.62, 1.30, 1.38 and 8.02 respectively. Rapid and stable growth was observed, among others, for BBLN1, BSO10, BSO13 and BSO14. The strains fulfilled the selection conditions and were identified based on the 16S rDNA sequences. BBLN1, BSO10, BSO13 were classified as Bacillus licheniformis, whereas BSO14 as Paenibacillus lactis.
Novelty and scientific contribution. We described cellulolytic activity and biochemical characteristics of many bacteria isolated from hot environments. We are also the first to report the cellulolytic activity of thermotolerant P. lactis. Described strains can be a source of new thermostable cellulases, which are extremely desirable in various branches of circular bioeconomy. |
| first_indexed | 2024-04-24T09:14:19Z |
| format | Article |
| id | doaj.art-e6d60c643d7b4f6da18cd6fe0b553053 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:14:19Z |
| publishDate | 2021-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-e6d60c643d7b4f6da18cd6fe0b5530532024-04-15T17:16:11ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062021-01-0159332533610.17113/ftb.59.03.21.7096Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformisKrzysztof Makowski0Martyna Leszczewicz1Natalia Broncel2Lidia Lipińska-Zubrycka3Adrian Głębski4Piotr Komorowski5Bogdan Walkowiak6Industrial Biotechnology Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandIndustrial Biotechnology Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandIndustrial Biotechnology Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandIndustrial Biotechnology Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandIndustrial Biotechnology Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandMolecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandMolecular and Nanostructural Biophysics Laboratory, Bionanopark Ltd., Dubois 114/116, Lodz, PolandResearch background. Cellulose is an ingredient of waste materials that can be converted to other valuable substances. This is possible provided that the polymer molecule is degraded to smaller particles and used as a carbon source by microorganisms. Because of the frequently applied methods of pretreatment of lignocellulosic materials, the cellulases derived from thermophilic microorganisms are particularly desirable. Experimental approach. We were looking for cellulolytic microorganisms able to grow at 50 °C and we described their morphological features and biochemical characteristics based on carboxymethyl cellulase (CMCase) activity and the API® ZYM system. The growth curves during incubation at 50 °C were examined using the BioLector® microbioreactor. Results and conclusions. Forty bacterial strains were isolated from fermenting hay, geothermal karst spring, hot spring and geothermal pond at 50 °C. The vast majority of the bacteria were Gram-positive and rod-shaped with the maximum growth temperature of at least 50 °C. We also demonstrated a large diversity of biochemical characteristics among the microorganisms. The CMCase activity was confirmed in 27 strains. Hydrolysis capacities were significant in bacterial strains: BBLN1, BSO6, BSO10, BSO13 and BSO14, and reached 2.74, 1.62, 1.30, 1.38 and 8.02 respectively. Rapid and stable growth was observed, among others, for BBLN1, BSO10, BSO13 and BSO14. The strains fulfilled the selection conditions and were identified based on the 16S rDNA sequences. BBLN1, BSO10, BSO13 were classified as Bacillus licheniformis, whereas BSO14 as Paenibacillus lactis. Novelty and scientific contribution. We described cellulolytic activity and biochemical characteristics of many bacteria isolated from hot environments. We are also the first to report the cellulolytic activity of thermotolerant P. lactis. Described strains can be a source of new thermostable cellulases, which are extremely desirable in various branches of circular bioeconomy.https://hrcak.srce.hr/file/384093Paenibacillus lactisBacillus licheniformiscellulolytic activitythermotolerant bacteriacarboxymethylcellulose (CMC)BioLector® microbioreactor |
| spellingShingle | Krzysztof Makowski Martyna Leszczewicz Natalia Broncel Lidia Lipińska-Zubrycka Adrian Głębski Piotr Komorowski Bogdan Walkowiak Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis Food Technology and Biotechnology Paenibacillus lactis Bacillus licheniformis cellulolytic activity thermotolerant bacteria carboxymethylcellulose (CMC) BioLector® microbioreactor |
| title | Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis |
| title_full | Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis |
| title_fullStr | Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis |
| title_full_unstemmed | Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis |
| title_short | Isolation, Biochemical Characterisation and Identification of Thermotolerant and Cellulolytic Paenibacillus lactis and Bacillus licheniformis |
| title_sort | isolation biochemical characterisation and identification of thermotolerant and cellulolytic paenibacillus lactis and bacillus licheniformis |
| topic | Paenibacillus lactis Bacillus licheniformis cellulolytic activity thermotolerant bacteria carboxymethylcellulose (CMC) BioLector® microbioreactor |
| url | https://hrcak.srce.hr/file/384093 |
| work_keys_str_mv | AT krzysztofmakowski isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT martynaleszczewicz isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT nataliabroncel isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT lidialipinskazubrycka isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT adriangłebski isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT piotrkomorowski isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis AT bogdanwalkowiak isolationbiochemicalcharacterisationandidentificationofthermotolerantandcellulolyticpaenibacilluslactisandbacilluslicheniformis |