Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice
This study aimed to explore the effects and mechanisms of maternal gestational diabetes mellitus (GDM) and selenium (Se) deficiency on the growth and glucose metabolism of offspring. Female C57BL/6J mice were divided into four groups as follows: a control group, a GDM group, a Se deficiency group, a...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/15/21/4519 |
| _version_ | 1797631527339687936 |
|---|---|
| author | Wenhui Xu Jiayu Gong Yifei Chen Yiru Chen Shutong Chen Yanyan Wu Yuan He Chenxu Li Haitao Yu Lin Xie |
| author_facet | Wenhui Xu Jiayu Gong Yifei Chen Yiru Chen Shutong Chen Yanyan Wu Yuan He Chenxu Li Haitao Yu Lin Xie |
| author_sort | Wenhui Xu |
| collection | DOAJ |
| description | This study aimed to explore the effects and mechanisms of maternal gestational diabetes mellitus (GDM) and selenium (Se) deficiency on the growth and glucose metabolism of offspring. Female C57BL/6J mice were divided into four groups as follows: a control group, a GDM group, a Se deficiency group, and a GDM with Se deficiency group. GDM animal models were established via S961. Pregnant mice fed their offspring until weaning. Then, offspring continued to be fed with a basic diet until adulthood. Body weight and fasting blood glucose were measured weekly. Se content, oxidative stress indicators, and the protein expression of the PI3K/Akt signaling pathway were detected. GDM increased susceptibility to obesity in lactating offspring, with gender differences observed in adult offspring. The effect of Se deficiency on SOD activity only appeared in female offspring during adulthood but was shown in male offspring during weaning though it disappeared during adulthood. GDM and Se deficiency increased the risk of abnormal glucose metabolism in female offspring from weaning to adulthood but gradually decreased in male offspring. The influence on the expression of PI3K/Akt signaling pathway-related proteins showed the same trend. GDM and Se deficiency affected the growth and glucose metabolism of offspring through oxidative stress and PI3K/Akt signaling pathway-related proteins, and gender differences existed. |
| first_indexed | 2024-03-11T11:24:50Z |
| format | Article |
| id | doaj.art-e6d6acfba2dd424fbdd118283f9f4c30 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T11:24:50Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-e6d6acfba2dd424fbdd118283f9f4c302023-11-10T15:09:28ZengMDPI AGNutrients2072-66432023-10-011521451910.3390/nu15214519Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J MiceWenhui Xu0Jiayu Gong1Yifei Chen2Yiru Chen3Shutong Chen4Yanyan Wu5Yuan He6Chenxu Li7Haitao Yu8Lin Xie9School of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaClinical Nutrition Department, Third Hospital of Jilin University, Changchun 130032, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaSchool of Public Health, Jilin University, Changchun 130012, ChinaThis study aimed to explore the effects and mechanisms of maternal gestational diabetes mellitus (GDM) and selenium (Se) deficiency on the growth and glucose metabolism of offspring. Female C57BL/6J mice were divided into four groups as follows: a control group, a GDM group, a Se deficiency group, and a GDM with Se deficiency group. GDM animal models were established via S961. Pregnant mice fed their offspring until weaning. Then, offspring continued to be fed with a basic diet until adulthood. Body weight and fasting blood glucose were measured weekly. Se content, oxidative stress indicators, and the protein expression of the PI3K/Akt signaling pathway were detected. GDM increased susceptibility to obesity in lactating offspring, with gender differences observed in adult offspring. The effect of Se deficiency on SOD activity only appeared in female offspring during adulthood but was shown in male offspring during weaning though it disappeared during adulthood. GDM and Se deficiency increased the risk of abnormal glucose metabolism in female offspring from weaning to adulthood but gradually decreased in male offspring. The influence on the expression of PI3K/Akt signaling pathway-related proteins showed the same trend. GDM and Se deficiency affected the growth and glucose metabolism of offspring through oxidative stress and PI3K/Akt signaling pathway-related proteins, and gender differences existed.https://www.mdpi.com/2072-6643/15/21/4519gestational diabetes mellitusoffspringglucosegrowth and development |
| spellingShingle | Wenhui Xu Jiayu Gong Yifei Chen Yiru Chen Shutong Chen Yanyan Wu Yuan He Chenxu Li Haitao Yu Lin Xie Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice Nutrients gestational diabetes mellitus offspring glucose growth and development |
| title | Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice |
| title_full | Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice |
| title_fullStr | Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice |
| title_full_unstemmed | Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice |
| title_short | Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice |
| title_sort | effects of gestational diabetes mellitus and selenium deficiency on the offspring growth and blood glucose mechanisms of c57bl 6j mice |
| topic | gestational diabetes mellitus offspring glucose growth and development |
| url | https://www.mdpi.com/2072-6643/15/21/4519 |
| work_keys_str_mv | AT wenhuixu effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT jiayugong effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT yifeichen effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT yiruchen effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT shutongchen effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT yanyanwu effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT yuanhe effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT chenxuli effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT haitaoyu effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice AT linxie effectsofgestationaldiabetesmellitusandseleniumdeficiencyontheoffspringgrowthandbloodglucosemechanismsofc57bl6jmice |