Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms
Simple, precise and economic batch and flow injection analysis (FIA)-spectrophotometric methods have been established for simultaneous determination of salbutamol sulfate (SLB) in bulk powder and pharmaceutical forms. Both methods based on diazotization coupling reaction of SLB with another drug co...
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| Format: | Article |
| Language: | English |
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College of Pharmacy University of Baghdad
2023-06-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1699 |
| _version_ | 1797802412232146944 |
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| author | Wasan Abdulamir Al-Uzri Mariam Jamal Hind Hadi |
| author_facet | Wasan Abdulamir Al-Uzri Mariam Jamal Hind Hadi |
| author_sort | Wasan Abdulamir Al-Uzri |
| collection | DOAJ |
| description |
Simple, precise and economic batch and flow injection analysis (FIA)-spectrophotometric methods have been established for simultaneous determination of salbutamol sulfate (SLB) in bulk powder and pharmaceutical forms. Both methods based on diazotization coupling reaction of SLB with another drug compound (sulfadimidine) as a safe and green diazotization agent in alkaline medium. At 444 nm, the maximum absorption of the orange azo-dye product was observed. A thorough investigation of all chemical and physical factors was conducted for batch and FIA procedures to achieve high sensitivity. Under the optimized experimental variables, SLB obeys Beer’s law in the concentration range of 0.25-4 and 10-100 μg/mL with limits of detection of 0.09 and 2.51 μg/mL for batch and FIA procedures respectively. The low percentage relative standard deviation of less than 1% (n=5) for both methods confirmed the applicability of these methods. Using F and t tests, a statistical comparison of the recommended approaches with the standard spectrophotometric method revealed no significant differences in accuracy or precision.
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| first_indexed | 2024-03-13T05:05:18Z |
| format | Article |
| id | doaj.art-e6d8647cbdde4a02b903a0fc979cff5e |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-03-13T05:05:18Z |
| publishDate | 2023-06-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-e6d8647cbdde4a02b903a0fc979cff5e2023-06-16T19:54:01ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972023-06-0132110.31351/vol32iss1pp45-52Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical FormsWasan Abdulamir Al-Uzri0Mariam Jamal1Hind Hadi2Department of Chemistry, College of Science, University of Baghdad, Baghdad, IraqMinistry of Education, Educational Rusafa Directorate IIDepartment of Chemistry, College of Science, University of Baghdad, Simple, precise and economic batch and flow injection analysis (FIA)-spectrophotometric methods have been established for simultaneous determination of salbutamol sulfate (SLB) in bulk powder and pharmaceutical forms. Both methods based on diazotization coupling reaction of SLB with another drug compound (sulfadimidine) as a safe and green diazotization agent in alkaline medium. At 444 nm, the maximum absorption of the orange azo-dye product was observed. A thorough investigation of all chemical and physical factors was conducted for batch and FIA procedures to achieve high sensitivity. Under the optimized experimental variables, SLB obeys Beer’s law in the concentration range of 0.25-4 and 10-100 μg/mL with limits of detection of 0.09 and 2.51 μg/mL for batch and FIA procedures respectively. The low percentage relative standard deviation of less than 1% (n=5) for both methods confirmed the applicability of these methods. Using F and t tests, a statistical comparison of the recommended approaches with the standard spectrophotometric method revealed no significant differences in accuracy or precision. https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1699Salbutamol, sulfadimidine, flow injection analysis, diazotization reaction |
| spellingShingle | Wasan Abdulamir Al-Uzri Mariam Jamal Hind Hadi Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms Iraqi Journal of Pharmaceutical Sciences Salbutamol, sulfadimidine, flow injection analysis, diazotization reaction |
| title | Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms |
| title_full | Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms |
| title_fullStr | Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms |
| title_full_unstemmed | Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms |
| title_short | Colorimetric Determination of Salbutamol Sulfate using Spectrophotometry-Continuous Flow Injection Technique in Pure and Pharmaceutical Forms |
| title_sort | colorimetric determination of salbutamol sulfate using spectrophotometry continuous flow injection technique in pure and pharmaceutical forms |
| topic | Salbutamol, sulfadimidine, flow injection analysis, diazotization reaction |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1699 |
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