Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i>
It has been reported that the mitochondrial carrier family proteins of AtMTM1 and AtMTM2 are necessary for manganese superoxide dismutase (MnSOD) activation in <i>Arabidopsis</i>, and are responsive to methyl viologen (MV)-induced oxidative stress. In this study, we showed that MnSOD act...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/5/619 |
| _version_ | 1797474128042655744 |
|---|---|
| author | Shu-Hsuan Hu Tsung-Luo Jinn |
| author_facet | Shu-Hsuan Hu Tsung-Luo Jinn |
| author_sort | Shu-Hsuan Hu |
| collection | DOAJ |
| description | It has been reported that the mitochondrial carrier family proteins of AtMTM1 and AtMTM2 are necessary for manganese superoxide dismutase (MnSOD) activation in <i>Arabidopsis</i>, and are responsive to methyl viologen (MV)-induced oxidative stress. In this study, we showed that MnSOD activity was enhanced specifically by Mn treatments. By using <i>AtMnSOD</i>-overexpressing and <i>AtMnSOD</i>-knockdown mutant plants treated with the widely used oxidative stressors including MV, NaCl, H<sub>2</sub>O<sub>2</sub>, and tert-butyl hydroperoxide (t-BH), we revealed that <i>Arabidopsis</i> MnSOD was crucial for root-growth control and superoxide scavenging ability. In addition, it has been reported that <i>E</i>. <i>coli</i> MnSOD activity is inhibited by Fe and that <i>MTM1</i>-mutated yeast cells exhibit elevated Fe content and decreased MnSOD activity, which can be restored by the Fe<sup>2+</sup>-specific chelator, bathophenanthroline disulfonate (BPS). However, we showed that BPS inhibited MnSOD activity in <i>AtMTM1</i> and <i>AtMTM2</i> single- and double-mutant protoplasts, implying that altered Fe homeostasis affected MnSOD activation through AtMTM1 and AtMTM2. Notably, we used inductively coupled plasma-optical emission spectrometry (ICP-OES) analysis to reveal an abnormal Fe/Mn ratio in the roots and shoots of <i>AtMTM1</i> and <i>AtMTM2</i> mutants under MV stress, indicating the importance of AtMTM1 in roots and AtMTM2 in shoots for maintaining Fe/Mn balance. |
| first_indexed | 2024-03-09T20:26:41Z |
| format | Article |
| id | doaj.art-e6d99744113845f0b95ee3f85fad8e40 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T20:26:41Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e6d99744113845f0b95ee3f85fad8e402023-11-23T23:35:35ZengMDPI AGPlants2223-77472022-02-0111561910.3390/plants11050619Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i>Shu-Hsuan Hu0Tsung-Luo Jinn1Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, TaiwanInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, TaiwanIt has been reported that the mitochondrial carrier family proteins of AtMTM1 and AtMTM2 are necessary for manganese superoxide dismutase (MnSOD) activation in <i>Arabidopsis</i>, and are responsive to methyl viologen (MV)-induced oxidative stress. In this study, we showed that MnSOD activity was enhanced specifically by Mn treatments. By using <i>AtMnSOD</i>-overexpressing and <i>AtMnSOD</i>-knockdown mutant plants treated with the widely used oxidative stressors including MV, NaCl, H<sub>2</sub>O<sub>2</sub>, and tert-butyl hydroperoxide (t-BH), we revealed that <i>Arabidopsis</i> MnSOD was crucial for root-growth control and superoxide scavenging ability. In addition, it has been reported that <i>E</i>. <i>coli</i> MnSOD activity is inhibited by Fe and that <i>MTM1</i>-mutated yeast cells exhibit elevated Fe content and decreased MnSOD activity, which can be restored by the Fe<sup>2+</sup>-specific chelator, bathophenanthroline disulfonate (BPS). However, we showed that BPS inhibited MnSOD activity in <i>AtMTM1</i> and <i>AtMTM2</i> single- and double-mutant protoplasts, implying that altered Fe homeostasis affected MnSOD activation through AtMTM1 and AtMTM2. Notably, we used inductively coupled plasma-optical emission spectrometry (ICP-OES) analysis to reveal an abnormal Fe/Mn ratio in the roots and shoots of <i>AtMTM1</i> and <i>AtMTM2</i> mutants under MV stress, indicating the importance of AtMTM1 in roots and AtMTM2 in shoots for maintaining Fe/Mn balance.https://www.mdpi.com/2223-7747/11/5/619Fe/Mn ratioMnSODmitochondrial carrier proteinMn transporterSODsuperoxide |
| spellingShingle | Shu-Hsuan Hu Tsung-Luo Jinn Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> Plants Fe/Mn ratio MnSOD mitochondrial carrier protein Mn transporter SOD superoxide |
| title | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> |
| title_full | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> |
| title_fullStr | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> |
| title_full_unstemmed | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> |
| title_short | Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in <i>Arabidopsis</i> |
| title_sort | impacts of mn fe and oxidative stressors on mnsod activation by atmtm1 and atmtm2 in i arabidopsis i |
| topic | Fe/Mn ratio MnSOD mitochondrial carrier protein Mn transporter SOD superoxide |
| url | https://www.mdpi.com/2223-7747/11/5/619 |
| work_keys_str_mv | AT shuhsuanhu impactsofmnfeandoxidativestressorsonmnsodactivationbyatmtm1andatmtm2iniarabidopsisi AT tsungluojinn impactsofmnfeandoxidativestressorsonmnsodactivationbyatmtm1andatmtm2iniarabidopsisi |