Circadian timing of injury-induced cell proliferation in zebrafish.
In certain vertebrates such as the zebrafish, most tissues and organs including the heart and central nervous system possess the remarkable ability to regenerate following severe injury. Both spatial and temporal control of cell proliferation and differentiation is essential for the successful repai...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3315524?pdf=render |
| _version_ | 1818277788458680320 |
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| author | Maria Laura Idda Elena Kage Jose Fernando Lopez-Olmeda Philipp Mracek Nicholas S Foulkes Daniela Vallone |
| author_facet | Maria Laura Idda Elena Kage Jose Fernando Lopez-Olmeda Philipp Mracek Nicholas S Foulkes Daniela Vallone |
| author_sort | Maria Laura Idda |
| collection | DOAJ |
| description | In certain vertebrates such as the zebrafish, most tissues and organs including the heart and central nervous system possess the remarkable ability to regenerate following severe injury. Both spatial and temporal control of cell proliferation and differentiation is essential for the successful repair and re-growth of damaged tissues. Here, using the regenerating adult zebrafish caudal fin as a model, we have demonstrated an involvement of the circadian clock in timing cell proliferation following injury. Using a BrdU incorporation assay with a short labeling period, we reveal high amplitude daily rhythms in S-phase in the epidermal cell layer of the fin under normal conditions. Peak numbers of S-phase cells occur at the end of the light period while lowest levels are observed at the end of the dark period. Remarkably, immediately following amputation the basal level of epidermal cell proliferation increases significantly with kinetics, depending upon the time of day when the amputation is performed. In sharp contrast, we failed to detect circadian rhythms of S-phase in the highly proliferative mesenchymal cells of the blastema. Subsequently, during the entire period of outgrowth of the new fin, elevated, cycling levels of epidermal cell proliferation persist. Thus, our results point to a preferential role for the circadian clock in the timing of epidermal cell proliferation in response to injury. |
| first_indexed | 2024-12-12T23:07:06Z |
| format | Article |
| id | doaj.art-e6d9cb46ea554d4eabbb1708d44ffa19 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:07:06Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e6d9cb46ea554d4eabbb1708d44ffa192022-12-22T00:08:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3420310.1371/journal.pone.0034203Circadian timing of injury-induced cell proliferation in zebrafish.Maria Laura IddaElena KageJose Fernando Lopez-OlmedaPhilipp MracekNicholas S FoulkesDaniela ValloneIn certain vertebrates such as the zebrafish, most tissues and organs including the heart and central nervous system possess the remarkable ability to regenerate following severe injury. Both spatial and temporal control of cell proliferation and differentiation is essential for the successful repair and re-growth of damaged tissues. Here, using the regenerating adult zebrafish caudal fin as a model, we have demonstrated an involvement of the circadian clock in timing cell proliferation following injury. Using a BrdU incorporation assay with a short labeling period, we reveal high amplitude daily rhythms in S-phase in the epidermal cell layer of the fin under normal conditions. Peak numbers of S-phase cells occur at the end of the light period while lowest levels are observed at the end of the dark period. Remarkably, immediately following amputation the basal level of epidermal cell proliferation increases significantly with kinetics, depending upon the time of day when the amputation is performed. In sharp contrast, we failed to detect circadian rhythms of S-phase in the highly proliferative mesenchymal cells of the blastema. Subsequently, during the entire period of outgrowth of the new fin, elevated, cycling levels of epidermal cell proliferation persist. Thus, our results point to a preferential role for the circadian clock in the timing of epidermal cell proliferation in response to injury.http://europepmc.org/articles/PMC3315524?pdf=render |
| spellingShingle | Maria Laura Idda Elena Kage Jose Fernando Lopez-Olmeda Philipp Mracek Nicholas S Foulkes Daniela Vallone Circadian timing of injury-induced cell proliferation in zebrafish. PLoS ONE |
| title | Circadian timing of injury-induced cell proliferation in zebrafish. |
| title_full | Circadian timing of injury-induced cell proliferation in zebrafish. |
| title_fullStr | Circadian timing of injury-induced cell proliferation in zebrafish. |
| title_full_unstemmed | Circadian timing of injury-induced cell proliferation in zebrafish. |
| title_short | Circadian timing of injury-induced cell proliferation in zebrafish. |
| title_sort | circadian timing of injury induced cell proliferation in zebrafish |
| url | http://europepmc.org/articles/PMC3315524?pdf=render |
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