A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing
This study aims to analyze the whole genome sequencing of <i>E. coli</i> F17 in antagonistic and susceptible Hu sheep lambs. The objective is to investigate the critical mutation loci in sheep and understand the genetic mechanism of sheep resistance to <i>E. coli</i> F17 at t...
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MDPI AG
2024-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/1/161 |
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| author | Yanjun Duan Pengwei Su Yifei Gu Xiaoyang Lv Xiukai Cao Shanhe Wang Zehu Yuan Wei Sun |
| author_facet | Yanjun Duan Pengwei Su Yifei Gu Xiaoyang Lv Xiukai Cao Shanhe Wang Zehu Yuan Wei Sun |
| author_sort | Yanjun Duan |
| collection | DOAJ |
| description | This study aims to analyze the whole genome sequencing of <i>E. coli</i> F17 in antagonistic and susceptible Hu sheep lambs. The objective is to investigate the critical mutation loci in sheep and understand the genetic mechanism of sheep resistance to <i>E. coli</i> F17 at the genome level. Antagonist and susceptible venous blood samples were collected from Hu sheep lambs for whole genome sequencing and whole genome association analysis. A total of 466 genes with significant SNPs (<i>p</i> < 1.0 × 10<sup>−3</sup>) were found. GO and KEGG enrichment analysis and protein interaction network analysis were performed on these genes, and preliminary investigations showed that SNPs on CTNNB1, CDH8, APOD, HCLS1, Tet2, MTSS1 and YAP1 genes may be associated with the antagonism and susceptibility of Hu sheep lambs to <i>E. coli</i> F17. There are still some shortcomings that have not been explored via in vivo and in vitro functional experiments of the candidate genes, which will be our next research work. This study provides genetic loci and candidate genes for resistance of Hu sheep lambs to <i>E. coli</i> F17 infection, and provides a genetic basis for breeding disease-resistant sheep. |
| first_indexed | 2024-03-08T15:12:50Z |
| format | Article |
| id | doaj.art-e6dca8e443d64484813a7b27aa209ddc |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T15:12:50Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e6dca8e443d64484813a7b27aa209ddc2024-01-10T14:50:31ZengMDPI AGAnimals2076-26152024-01-0114116110.3390/ani14010161A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome SequencingYanjun Duan0Pengwei Su1Yifei Gu2Xiaoyang Lv3Xiukai Cao4Shanhe Wang5Zehu Yuan6Wei Sun7College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThis study aims to analyze the whole genome sequencing of <i>E. coli</i> F17 in antagonistic and susceptible Hu sheep lambs. The objective is to investigate the critical mutation loci in sheep and understand the genetic mechanism of sheep resistance to <i>E. coli</i> F17 at the genome level. Antagonist and susceptible venous blood samples were collected from Hu sheep lambs for whole genome sequencing and whole genome association analysis. A total of 466 genes with significant SNPs (<i>p</i> < 1.0 × 10<sup>−3</sup>) were found. GO and KEGG enrichment analysis and protein interaction network analysis were performed on these genes, and preliminary investigations showed that SNPs on CTNNB1, CDH8, APOD, HCLS1, Tet2, MTSS1 and YAP1 genes may be associated with the antagonism and susceptibility of Hu sheep lambs to <i>E. coli</i> F17. There are still some shortcomings that have not been explored via in vivo and in vitro functional experiments of the candidate genes, which will be our next research work. This study provides genetic loci and candidate genes for resistance of Hu sheep lambs to <i>E. coli</i> F17 infection, and provides a genetic basis for breeding disease-resistant sheep.https://www.mdpi.com/2076-2615/14/1/161whole genome sequencing<i>Escherichia coli</i> F17Hu sheep lambsantagonistic phenotypesusceptible phenotype |
| spellingShingle | Yanjun Duan Pengwei Su Yifei Gu Xiaoyang Lv Xiukai Cao Shanhe Wang Zehu Yuan Wei Sun A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing Animals whole genome sequencing <i>Escherichia coli</i> F17 Hu sheep lambs antagonistic phenotype susceptible phenotype |
| title | A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing |
| title_full | A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing |
| title_fullStr | A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing |
| title_full_unstemmed | A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing |
| title_short | A Study of the Resistance of Hu Sheep Lambs to <i>Escherichia coli</i> F17 Based on Whole Genome Sequencing |
| title_sort | study of the resistance of hu sheep lambs to i escherichia coli i f17 based on whole genome sequencing |
| topic | whole genome sequencing <i>Escherichia coli</i> F17 Hu sheep lambs antagonistic phenotype susceptible phenotype |
| url | https://www.mdpi.com/2076-2615/14/1/161 |
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