| סיכום: | The development of efficient, reliable, and sensitive dopamine detection methods has attracted much attention. In this paper, vancomycin-stabilized platinum nanoparticles (Van-Pt<sub>n</sub> NPs, n = 0.5, 1, 2) were prepared by the biological template method, where n represented the molar ratio of vancomycin to Pt. The results show that Van-Pt<sub>2</sub> NPs had oxidase-like activity and peroxidase-like activity, and the mechanism was due to the generation of reactive oxygen <sup>1</sup>O<sub>2</sub> and OH. Van-Pt<sub>2</sub> NPs exhibited good temperature stability, storage stability, and salt solution stability. Furthermore, Van-Pt<sub>2</sub> NPs had almost no cytotoxicity to A549 cells. More importantly, the colorimetric detection of DA in human serum samples was performed based on the oxidase-like activity of Van-Pt<sub>2</sub> NPs. The linear range of DA detection was 10–700 μM, and the detection limit was 0.854 μM. This study establishes a rapid and reliable method for the detection of dopamine and extends the application of biosynthetic nanoparticles in the field of biosensing.
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