Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males
Summary: In internal fertilization animals, reproductive success depends on maintaining copulation until gametes are transported from male to female. In Drosophila melanogaster, mechanosensation in males likely contributes to copulation maintenance, but its molecular underpinning remains to be ident...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223006946 |
| _version_ | 1797844295279968256 |
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| author | Hayato M. Yamanouchi Ryoya Tanaka Azusa Kamikouchi |
| author_facet | Hayato M. Yamanouchi Ryoya Tanaka Azusa Kamikouchi |
| author_sort | Hayato M. Yamanouchi |
| collection | DOAJ |
| description | Summary: In internal fertilization animals, reproductive success depends on maintaining copulation until gametes are transported from male to female. In Drosophila melanogaster, mechanosensation in males likely contributes to copulation maintenance, but its molecular underpinning remains to be identified. Here we show that the mechanosensory gene piezo and its’ expressing neurons are responsible for copulation maintenance. An RNA-seq database search and subsequent mutant analysis revealed the importance of piezo for maintaining male copulation posture. piezo-GAL4-positive signals were found in the sensory neurons of male genitalia bristles, and optogenetic inhibition of piezo-expressing neurons in the posterior side of the male body during copulation destabilized posture and terminated copulation. Our findings suggest that the mechanosensory system of male genitalia through Piezo channels plays a key role in copulation maintenance and indicate that Piezo may increase male fitness during copulation in flies. |
| first_indexed | 2024-04-09T17:21:05Z |
| format | Article |
| id | doaj.art-e6e3a9bcfdbd45e994d7320f71a2c04a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T17:21:05Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e6e3a9bcfdbd45e994d7320f71a2c04a2023-04-19T04:23:14ZengElsevieriScience2589-00422023-05-01265106617Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila malesHayato M. Yamanouchi0Ryoya Tanaka1Azusa Kamikouchi2Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, JapanGraduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan; Corresponding authorGraduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Aichi 464-8602, Japan; Corresponding authorSummary: In internal fertilization animals, reproductive success depends on maintaining copulation until gametes are transported from male to female. In Drosophila melanogaster, mechanosensation in males likely contributes to copulation maintenance, but its molecular underpinning remains to be identified. Here we show that the mechanosensory gene piezo and its’ expressing neurons are responsible for copulation maintenance. An RNA-seq database search and subsequent mutant analysis revealed the importance of piezo for maintaining male copulation posture. piezo-GAL4-positive signals were found in the sensory neurons of male genitalia bristles, and optogenetic inhibition of piezo-expressing neurons in the posterior side of the male body during copulation destabilized posture and terminated copulation. Our findings suggest that the mechanosensory system of male genitalia through Piezo channels plays a key role in copulation maintenance and indicate that Piezo may increase male fitness during copulation in flies.http://www.sciencedirect.com/science/article/pii/S2589004223006946MechanobiologyMolecular physiologyBehavioral neuroscience |
| spellingShingle | Hayato M. Yamanouchi Ryoya Tanaka Azusa Kamikouchi Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males iScience Mechanobiology Molecular physiology Behavioral neuroscience |
| title | Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males |
| title_full | Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males |
| title_fullStr | Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males |
| title_full_unstemmed | Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males |
| title_short | Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in Drosophila males |
| title_sort | piezo mediated mechanosensation contributes to stabilizing copulation posture and reproductive success in drosophila males |
| topic | Mechanobiology Molecular physiology Behavioral neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223006946 |
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