Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits
Inulin as a commercial prebiotic could selectively promote the growth of beneficial gut microbes such as lactic acid bacteria (LAB). Whether LAB in rabbit gut possesses the capability to metabolize and utilize inulin is little known. Therefore, this study recovered 94 LAB strains from neonate rabbit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.719927/full |
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| author | Yuan-ting Zhu Shuang-ming Yue Rui-tong Li Shi-xiu Qiu Zhen-Ying Xu Yi Wu Jin Yao Yong Zuo Ke-juan Li Yang Li |
| author_facet | Yuan-ting Zhu Shuang-ming Yue Rui-tong Li Shi-xiu Qiu Zhen-Ying Xu Yi Wu Jin Yao Yong Zuo Ke-juan Li Yang Li |
| author_sort | Yuan-ting Zhu |
| collection | DOAJ |
| description | Inulin as a commercial prebiotic could selectively promote the growth of beneficial gut microbes such as lactic acid bacteria (LAB). Whether LAB in rabbit gut possesses the capability to metabolize and utilize inulin is little known. Therefore, this study recovered 94 LAB strains from neonate rabbits and found that only 29% (28/94) could metabolize inulin with both species- and strain-specificity. The most vigorous inulin-degrading strain, Lacticaseibacillus paracasei YT170, could efficiently utilize both short-chain and long-chain components through thin-layer chromatography analysis. From genomic analysis, a predicted fosRABCDXE operon encoding putative cell wall-anchored fructan β-fructosidase, five fructose-transporting proteins and a pts1BCA operon encoding putative β-fructofuranosidase and sucrose-specific IIBCA components were linked to long-chain and short-chain inulin utilization respectively. This study provides a mechanistic rationale for effect of inulin administration on rabbits and lays a foundation for synbiotic applications aimed at modulating the intestinal microbiota of young rabbits. |
| first_indexed | 2024-12-22T02:06:00Z |
| format | Article |
| id | doaj.art-e6e3efcaec8248c3856b04a90e954d10 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-22T02:06:00Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-e6e3efcaec8248c3856b04a90e954d102022-12-21T18:42:32ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-10-01810.3389/fvets.2021.719927719927Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young RabbitsYuan-ting Zhu0Shuang-ming Yue1Rui-tong Li2Shi-xiu Qiu3Zhen-Ying Xu4Yi Wu5Jin Yao6Yong Zuo7Ke-juan Li8Yang Li9College of Life Science, Sichuan Normal University, Chengdu, ChinaDepartment of Bioengineering, Sichuan Water Conservancy College, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaChengdu Academy of Agriculture and Forestry Sciences, Chengdu, ChinaChengdu Academy of Agriculture and Forestry Sciences, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaCollege of Life Science, Sichuan Normal University, Chengdu, ChinaInulin as a commercial prebiotic could selectively promote the growth of beneficial gut microbes such as lactic acid bacteria (LAB). Whether LAB in rabbit gut possesses the capability to metabolize and utilize inulin is little known. Therefore, this study recovered 94 LAB strains from neonate rabbits and found that only 29% (28/94) could metabolize inulin with both species- and strain-specificity. The most vigorous inulin-degrading strain, Lacticaseibacillus paracasei YT170, could efficiently utilize both short-chain and long-chain components through thin-layer chromatography analysis. From genomic analysis, a predicted fosRABCDXE operon encoding putative cell wall-anchored fructan β-fructosidase, five fructose-transporting proteins and a pts1BCA operon encoding putative β-fructofuranosidase and sucrose-specific IIBCA components were linked to long-chain and short-chain inulin utilization respectively. This study provides a mechanistic rationale for effect of inulin administration on rabbits and lays a foundation for synbiotic applications aimed at modulating the intestinal microbiota of young rabbits.https://www.frontiersin.org/articles/10.3389/fvets.2021.719927/fullinulinprebioticrabbitlacticaseibacillus paracaseisynbiotic |
| spellingShingle | Yuan-ting Zhu Shuang-ming Yue Rui-tong Li Shi-xiu Qiu Zhen-Ying Xu Yi Wu Jin Yao Yong Zuo Ke-juan Li Yang Li Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits Frontiers in Veterinary Science inulin prebiotic rabbit lacticaseibacillus paracasei synbiotic |
| title | Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits |
| title_full | Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits |
| title_fullStr | Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits |
| title_full_unstemmed | Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits |
| title_short | Prebiotics Inulin Metabolism by Lactic Acid Bacteria From Young Rabbits |
| title_sort | prebiotics inulin metabolism by lactic acid bacteria from young rabbits |
| topic | inulin prebiotic rabbit lacticaseibacillus paracasei synbiotic |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.719927/full |
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