Influence of the medium on production of trichoderma agent for biocontrol of maize fungal diseases
Maize is important agricultural crop that is susceptible to infections by various phytopathogenic fungi. Maize grain as a rich source of nutrients represents a very good medium for the development of toxic fungi from the different genera. Synthetic fungicides are generally used to protect p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Technology, Novi Sad
2022-01-01
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| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2022/1450-71882253253M.pdf |
| Summary: | Maize is important agricultural crop that is susceptible to infections by
various phytopathogenic fungi. Maize grain as a rich source of nutrients
represents a very good medium for the development of toxic fungi from the
different genera. Synthetic fungicides are generally used to protect plants
from various pathogenic fungi. However, this practice increases the risk of
fungicide resistance and environmental degradation of agroecosystems. The
use of Trichoderma isolates in biological control is an alternative
strategy, particularly for the problems of resistance and environmental
pollution, thus improving the economic and biological sustainability of
agroecosystems. In this paper, the effect of medium composition on the
production of Trichoderma harzianum biocontrol agent effective against maize
pathogens, Helminthosporium carbonum and Penicillium sp., was examined.
Results of Scheffe multiple range test showed that the best medium for
production of T. harzianum bioagent effective against H. carbonum and
Penicillium sp. contains soluble starch as carbon source and soybean meal as
nitrogen source. By applying this medium formulation, maximal inhibition
zone diameters of 54.33 mm and 24.67 mm was registered in vitro for H.
carbonum and Penicillium sp., respectively. Production of Trichoderma
bioagent was further tested on the selected medium in a ten times larger
volume, Woulff bottle, in which the success of this bioprocess was
confirmed. The results obtained in an increased volume on a selected medium
showed that this bioprocess can be shortened to 84 h, which contributes to
the economy of the bioprocess. |
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| ISSN: | 1450-7188 2406-095X |