Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars
The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most destructive insect pests, feeding exclusively on wild and cultivated cruciferous species. The attacked plants produce considerable amount of glucosinolates in response to insects’ feeding. Herein, we studied...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2020-12-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-31365-en.html |
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| author | Yaghoub Fathipour Roja Kianpour Abdoolnabi Bagheri Javad Karimzadeh Vahdi Hosseini Naveh Mohammad Mehrabadi |
| author_facet | Yaghoub Fathipour Roja Kianpour Abdoolnabi Bagheri Javad Karimzadeh Vahdi Hosseini Naveh Mohammad Mehrabadi |
| author_sort | Yaghoub Fathipour |
| collection | DOAJ |
| description | The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most destructive insect pests, feeding exclusively on wild and cultivated cruciferous species. The attacked plants produce considerable amount of glucosinolates in response to insects’ feeding. Herein, we studied digestive activities of P. xylostella on four different genotypes of family Brassicaceae including two canola cultivars (SLM046 and RGS003) and two cabbage cultivars (Green-Cornet and Glob-Master). The highest proteolytic and amylolytic activities of P. xylostella were observed on Green-Cornet and the lowest occurred on RGS003 and Glob-Master, respectively. The highest activity of α-glucosidase and β-glucosidases were observed on Green-Cornet and SLM046 and the lowest was observed on Glob-Master and RGS003. The zymogram analysis revealed different isozymes of protease, trypsin-like and α-amylase in the midgut extract of P. xylostella. Activity of the above mentioned isozymes was inhibited in larvae feeding on RGS003 and Glob Master as resistant host cultivars. Also, larvae feeding on the resistant genotypes showed more glucosidase activities, indicating possibility of high glycosinolate existence in the resistant genotypes. By these results we can state that host plant property can affect insect digestive physiology through inhibiting digestive enzyme activities. These findings provide insights into the direct effects of host plants on insect physiology which are conducive to change in insect fitness. |
| first_indexed | 2024-04-12T17:28:41Z |
| format | Article |
| id | doaj.art-e6e8560deed84070a261830c41d2eab4 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-12T17:28:41Z |
| publishDate | 2020-12-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-e6e8560deed84070a261830c41d2eab42022-12-22T03:23:13ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2020-12-01916579Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivarsYaghoub Fathipour0Roja Kianpour1Abdoolnabi Bagheri2Javad Karimzadeh3Vahdi Hosseini Naveh4Mohammad Mehrabadi5 Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran. Plant Protection Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Isfahan, Iran. Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most destructive insect pests, feeding exclusively on wild and cultivated cruciferous species. The attacked plants produce considerable amount of glucosinolates in response to insects’ feeding. Herein, we studied digestive activities of P. xylostella on four different genotypes of family Brassicaceae including two canola cultivars (SLM046 and RGS003) and two cabbage cultivars (Green-Cornet and Glob-Master). The highest proteolytic and amylolytic activities of P. xylostella were observed on Green-Cornet and the lowest occurred on RGS003 and Glob-Master, respectively. The highest activity of α-glucosidase and β-glucosidases were observed on Green-Cornet and SLM046 and the lowest was observed on Glob-Master and RGS003. The zymogram analysis revealed different isozymes of protease, trypsin-like and α-amylase in the midgut extract of P. xylostella. Activity of the above mentioned isozymes was inhibited in larvae feeding on RGS003 and Glob Master as resistant host cultivars. Also, larvae feeding on the resistant genotypes showed more glucosidase activities, indicating possibility of high glycosinolate existence in the resistant genotypes. By these results we can state that host plant property can affect insect digestive physiology through inhibiting digestive enzyme activities. These findings provide insights into the direct effects of host plants on insect physiology which are conducive to change in insect fitness.http://jcp.modares.ac.ir/article-3-31365-en.htmlbrassicaceaedigestive enzymesplant resistanceplutella xylostella |
| spellingShingle | Yaghoub Fathipour Roja Kianpour Abdoolnabi Bagheri Javad Karimzadeh Vahdi Hosseini Naveh Mohammad Mehrabadi Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars Journal of Crop Protection brassicaceae digestive enzymes plant resistance plutella xylostella |
| title | Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars |
| title_full | Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars |
| title_fullStr | Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars |
| title_full_unstemmed | Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars |
| title_short | Targeting Plutella xylostella digestive enzymes by applying resistant Brassicaceae host cultivars |
| title_sort | targeting plutella xylostella digestive enzymes by applying resistant brassicaceae host cultivars |
| topic | brassicaceae digestive enzymes plant resistance plutella xylostella |
| url | http://jcp.modares.ac.ir/article-3-31365-en.html |
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