Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women
Introduction: Many new studies related to peri-implantation period and maintenance of pregnancy suggest that microRNAs have fundamentally role in regulating critical pathways in early pregnancy loss and implantation failure. This study was performed with aim to evaluate the possible association betw...
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| Format: | Article |
| Language: | fas |
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Mashhad University of Medical Sciences
2020-10-01
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| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| Subjects: | |
| Online Access: | https://ijogi.mums.ac.ir/article_17292_d3647e587e1a4eb351ebefa88d556bf7.pdf |
| _version_ | 1811328747849121792 |
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| author | Sara Nemati Vahedi Babak Kheirkhah Ali Akbar Malekirad |
| author_facet | Sara Nemati Vahedi Babak Kheirkhah Ali Akbar Malekirad |
| author_sort | Sara Nemati Vahedi |
| collection | DOAJ |
| description | Introduction: Many new studies related to peri-implantation period and maintenance of pregnancy suggest that microRNAs have fundamentally role in regulating critical pathways in early pregnancy loss and implantation failure. This study was performed with aim to evaluate the possible association between has-miR-125 T>C polymorphism and susceptibility to idiopathic recurrent pregnancy loss (RPL).
Methods: In this case-control study, 116 females with at least two or more recurrent pregnancy loss and 89 healthy women with a history of two successful pregnancies and without a history of miscarriage were evaluated. DNA molecule was extracted by RGDE method and genotyping was performed by ARMS Tetra method. Data were analyzed by SPSS software (version 22) and Chi-squared test. P<0.05 was considered statistically significant.
Results: The frequency of TT, TC and CC genotypes of rs12976445 polymorphism were 50.86%, 42.25% and 6.89% and in women with RPL and were 58.43%, 39.33% and 2.24% in healthy women. There was no significant relationship between rs12976445 polymorphism and recurrent pregnancy loss (P=0.238).
Conclusion: No significant association was found between rs12976445 polymorphism and RPL. |
| first_indexed | 2024-04-13T15:31:34Z |
| format | Article |
| id | doaj.art-e6e8d6dab9594264b44a0345285aa44a |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-04-13T15:31:34Z |
| publishDate | 2020-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-e6e8d6dab9594264b44a0345285aa44a2022-12-22T02:41:23ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632020-10-01238374810.22038/ijogi.2020.1729217292Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian womenSara Nemati Vahedi0Babak Kheirkhah1Ali Akbar Malekirad2PhD student in Molecular Cell Biology, Faculty of Basic Sciences, Islamic Azad University Sirjan Branch, Sirjan, Iran.Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University Baft Branch, Baft, Iran.Associate Professor, Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.Introduction: Many new studies related to peri-implantation period and maintenance of pregnancy suggest that microRNAs have fundamentally role in regulating critical pathways in early pregnancy loss and implantation failure. This study was performed with aim to evaluate the possible association between has-miR-125 T>C polymorphism and susceptibility to idiopathic recurrent pregnancy loss (RPL). Methods: In this case-control study, 116 females with at least two or more recurrent pregnancy loss and 89 healthy women with a history of two successful pregnancies and without a history of miscarriage were evaluated. DNA molecule was extracted by RGDE method and genotyping was performed by ARMS Tetra method. Data were analyzed by SPSS software (version 22) and Chi-squared test. P<0.05 was considered statistically significant. Results: The frequency of TT, TC and CC genotypes of rs12976445 polymorphism were 50.86%, 42.25% and 6.89% and in women with RPL and were 58.43%, 39.33% and 2.24% in healthy women. There was no significant relationship between rs12976445 polymorphism and recurrent pregnancy loss (P=0.238). Conclusion: No significant association was found between rs12976445 polymorphism and RPL.https://ijogi.mums.ac.ir/article_17292_d3647e587e1a4eb351ebefa88d556bf7.pdfmir-125recurrent pregnancy loss (rpl)single nucleotide polymorphism (snp)tetra -arms pcr |
| spellingShingle | Sara Nemati Vahedi Babak Kheirkhah Ali Akbar Malekirad Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān mir-125 recurrent pregnancy loss (rpl) single nucleotide polymorphism (snp) tetra -arms pcr |
| title | Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women |
| title_full | Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women |
| title_fullStr | Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women |
| title_full_unstemmed | Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women |
| title_short | Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women |
| title_sort | association between polymorphism of has mir 125 rs12976445 and susceptibility to idiopathic recurrent pregnancy loss in iranian women |
| topic | mir-125 recurrent pregnancy loss (rpl) single nucleotide polymorphism (snp) tetra -arms pcr |
| url | https://ijogi.mums.ac.ir/article_17292_d3647e587e1a4eb351ebefa88d556bf7.pdf |
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