Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose
The integration of lipid metabolism in the splanchnic bed and in subcutaneous adipose tissue before and after ingestion of a 75 g glucose load was studied by Fick's principle in seven healthy subjects. Six additional subjects were studied during a hyperinsulinemic euglycemic clamp. Release of n...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1999-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520324275 |
| _version_ | 1818731647168675840 |
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| author | Jens Bülow Lene Simonsen David Wiggins Sandy M. Humphreys Keith N. Frayn David Powell Geoffrey F. Gibbons |
| author_facet | Jens Bülow Lene Simonsen David Wiggins Sandy M. Humphreys Keith N. Frayn David Powell Geoffrey F. Gibbons |
| author_sort | Jens Bülow |
| collection | DOAJ |
| description | The integration of lipid metabolism in the splanchnic bed and in subcutaneous adipose tissue before and after ingestion of a 75 g glucose load was studied by Fick's principle in seven healthy subjects. Six additional subjects were studied during a hyperinsulinemic euglycemic clamp. Release of non-esterified fatty acids (NEFA) from adipose tissue and splanchnic NEFA extraction followed a similar time-course after oral glucose, and there was a highly significant relationship between adipose tissue NEFA release and splanchnic NEFA uptake. There was no immediate inhibition of splanchnic very low density lipoprotein (VLDL)-triacylglycerol (TAG) output when plasma insulin levels increased after glucose. Adipose tissue extraction of VLDL-TAG tended to vary in time in a manner similar to splanchnic VLDL-TAG output and the two were significantly related. The area-under-curves (AUC) for splanchnic extraction of NEFA was significantly lower than that for output of VLDL, implying depletion of hepatic TAG stores during the experiment. In the hyperinsulinemic clamp experiments, there was on average suppression of splanchnic VLDL-TAG output although between-person variability was marked. This suppression could be explained by a very low supply of NEFA during the clamp. We conclude that there is an integrated pattern of metabolism in splanchnic and adipose tissues in the postabsorptive and post-glucose states. Flux of NEFA from adipose tissue drives splanchnic NEFA uptake. Splanchnic VLDL-TAG secretion appears to be regulated by a number of factors and in turn controls TAG extraction in adipose tissue. Insulin does not seem to play a key role in the acute regulation of hepatic VLDL metabolism under these particular conditions in vivo.—Bülow, J., L. Simonsen, D. Wiggins, S. M. Humphreys, K. N. Frayn, D. Powell, and G. F. Gibbons. Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose. J. Lipid Res. 1999. 40: 2034–2043. |
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| format | Article |
| id | doaj.art-e6eaa57549b74bddb233aa29345580ec |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T23:21:00Z |
| publishDate | 1999-11-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-e6eaa57549b74bddb233aa29345580ec2022-12-21T21:28:54ZengElsevierJournal of Lipid Research0022-22751999-11-01401120342043Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucoseJens Bülow0Lene Simonsen1David Wiggins2Sandy M. Humphreys3Keith N. Frayn4David Powell5Geoffrey F. Gibbons6To whom correspondence should be addressed.; Department of Clinical Physiology, Bispebjerg Hospital, DK-2400, Copenhagen NV, DenmarkDepartment of Clinical Physiology, Bispebjerg Hospital, DK-2400, Copenhagen NV, DenmarkMetabolic Research Laboratory, Radcliffe Infirmary, Oxford, OX2 6HE, UKOxford Lipid Metabolism Group, Radcliffe Infirmary, Oxford, OX2 6HE, UKOxford Lipid Metabolism Group, Radcliffe Infirmary, Oxford, OX2 6HE, UKMetabolic Research Laboratory, Radcliffe Infirmary, Oxford, OX2 6HE, UKMetabolic Research Laboratory, Radcliffe Infirmary, Oxford, OX2 6HE, UKThe integration of lipid metabolism in the splanchnic bed and in subcutaneous adipose tissue before and after ingestion of a 75 g glucose load was studied by Fick's principle in seven healthy subjects. Six additional subjects were studied during a hyperinsulinemic euglycemic clamp. Release of non-esterified fatty acids (NEFA) from adipose tissue and splanchnic NEFA extraction followed a similar time-course after oral glucose, and there was a highly significant relationship between adipose tissue NEFA release and splanchnic NEFA uptake. There was no immediate inhibition of splanchnic very low density lipoprotein (VLDL)-triacylglycerol (TAG) output when plasma insulin levels increased after glucose. Adipose tissue extraction of VLDL-TAG tended to vary in time in a manner similar to splanchnic VLDL-TAG output and the two were significantly related. The area-under-curves (AUC) for splanchnic extraction of NEFA was significantly lower than that for output of VLDL, implying depletion of hepatic TAG stores during the experiment. In the hyperinsulinemic clamp experiments, there was on average suppression of splanchnic VLDL-TAG output although between-person variability was marked. This suppression could be explained by a very low supply of NEFA during the clamp. We conclude that there is an integrated pattern of metabolism in splanchnic and adipose tissues in the postabsorptive and post-glucose states. Flux of NEFA from adipose tissue drives splanchnic NEFA uptake. Splanchnic VLDL-TAG secretion appears to be regulated by a number of factors and in turn controls TAG extraction in adipose tissue. Insulin does not seem to play a key role in the acute regulation of hepatic VLDL metabolism under these particular conditions in vivo.—Bülow, J., L. Simonsen, D. Wiggins, S. M. Humphreys, K. N. Frayn, D. Powell, and G. F. Gibbons. Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose. J. Lipid Res. 1999. 40: 2034–2043.http://www.sciencedirect.com/science/article/pii/S0022227520324275very low density lipoproteinsapolipoprotein Bparticle sizesplanchnic secretionadipose tissue uptakemetabolic cycling |
| spellingShingle | Jens Bülow Lene Simonsen David Wiggins Sandy M. Humphreys Keith N. Frayn David Powell Geoffrey F. Gibbons Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose Journal of Lipid Research very low density lipoproteins apolipoprotein B particle size splanchnic secretion adipose tissue uptake metabolic cycling |
| title | Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| title_full | Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| title_fullStr | Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| title_full_unstemmed | Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| title_short | Co-ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| title_sort | co ordination of hepatic and adipose tissue lipid metabolism after oral glucose |
| topic | very low density lipoproteins apolipoprotein B particle size splanchnic secretion adipose tissue uptake metabolic cycling |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520324275 |
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