A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1
MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that negatively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is conserved in invert...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922009520 |
| _version_ | 1828288667255308288 |
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| author | Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim |
| author_facet | Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim |
| author_sort | Sharvin Manickam |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that negatively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is conserved in invertebrates. miRNAs play key roles in gene regulation, cell proliferation, cell death, cell differentiation and cell developmental homeostasis in multicellular organisms. Its role in various human diseases is continuously being studied. miRNAs also found out to be crucial in maintaining stem cell niche in D. melanogaster gonads. We have identified that ectopic overexpression of miR-2b-1 of D. melanogaster causes testicular bulging (a tumour like phenotype) in 3-5 days old adult flies. Hence, we have performed a transcriptomic (RNA-seq) analysis to understand the role of miR-2b-1 in the development, maintenance, and differentiation of D. melanogaster adult testis stem cells. Data are available from GEO (accession number GSE211399). |
| first_indexed | 2024-04-13T10:08:02Z |
| format | Article |
| id | doaj.art-e6eb771cd2a04ce9a6bf4365b89ca25a |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-13T10:08:02Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-e6eb771cd2a04ce9a6bf4365b89ca25a2022-12-22T02:51:01ZengElsevierData in Brief2352-34092022-12-0145108748A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1Sharvin Manickam0Shallinie Thangadurai1Azali Azlan2Zarina Amin3Ghows Azzam4Mardani Abdul Halim5School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Penang 11800, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia; Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, Kajang, Selangor 43000, Malaysia; Corresponding author.Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, Malaysia; Corresponding author.MicroRNAs (miRNAs) are short non-coding single-stranded RNAs with approximately 22 nucleotides in length that negatively regulate the mRNA translation of a target gene. MiR-2b-1 belongs to the largest miR-2 family in Drosophila melanogaster with 8 members and this miRNA family is conserved in invertebrates. miRNAs play key roles in gene regulation, cell proliferation, cell death, cell differentiation and cell developmental homeostasis in multicellular organisms. Its role in various human diseases is continuously being studied. miRNAs also found out to be crucial in maintaining stem cell niche in D. melanogaster gonads. We have identified that ectopic overexpression of miR-2b-1 of D. melanogaster causes testicular bulging (a tumour like phenotype) in 3-5 days old adult flies. Hence, we have performed a transcriptomic (RNA-seq) analysis to understand the role of miR-2b-1 in the development, maintenance, and differentiation of D. melanogaster adult testis stem cells. Data are available from GEO (accession number GSE211399).http://www.sciencedirect.com/science/article/pii/S2352340922009520RNA-seqTranscriptomicsDrosophila melanogasterTesticular bulgingTestesmiR-2b-1 |
| spellingShingle | Sharvin Manickam Shallinie Thangadurai Azali Azlan Zarina Amin Ghows Azzam Mardani Abdul Halim A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 Data in Brief RNA-seq Transcriptomics Drosophila melanogaster Testicular bulging Testes miR-2b-1 |
| title | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_full | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_fullStr | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_full_unstemmed | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_short | A transcriptomic (RNA-seq) analysis of Drosophila melanogaster adult testes overexpressing microRNA-2b-1 |
| title_sort | transcriptomic rna seq analysis of drosophila melanogaster adult testes overexpressing microrna 2b 1 |
| topic | RNA-seq Transcriptomics Drosophila melanogaster Testicular bulging Testes miR-2b-1 |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922009520 |
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