Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging
Summary: Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit mol...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-09-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300514 |
| _version_ | 1818241196609241088 |
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| author | Madhulika Tripathi Paul Michael Yen Brijesh Kumar Singh |
| author_facet | Madhulika Tripathi Paul Michael Yen Brijesh Kumar Singh |
| author_sort | Madhulika Tripathi |
| collection | DOAJ |
| description | Summary: Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These senescent AML12 cells should be a useful in vitro model to study the metabolic effects of aging in the liver.For complete details on the use and execution of this protocol, please refer to Singh et al. (2020). |
| first_indexed | 2024-12-12T13:25:30Z |
| format | Article |
| id | doaj.art-e6ee686542c84003b84e890e1d30daf6 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T13:25:30Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-e6ee686542c84003b84e890e1d30daf62022-12-22T00:23:12ZengElsevierSTAR Protocols2666-16672020-09-0112100064Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic AgingMadhulika Tripathi0Paul Michael Yen1Brijesh Kumar Singh2Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, SingaporeCardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore; Duke University School of Medicine, Durham, NC, USACardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore; Corresponding authorSummary: Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These senescent AML12 cells should be a useful in vitro model to study the metabolic effects of aging in the liver.For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720300514 |
| spellingShingle | Madhulika Tripathi Paul Michael Yen Brijesh Kumar Singh Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging STAR Protocols |
| title | Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging |
| title_full | Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging |
| title_fullStr | Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging |
| title_full_unstemmed | Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging |
| title_short | Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging |
| title_sort | protocol to generate senescent cells from the mouse hepatic cell line aml12 to study hepatic aging |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300514 |
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