Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning
Abstract The aim of this study was to determine the possible impact of early socialization and an enriched neonatal environment to improve adaptation of piglets to weaning. We hypothesized that changes in the microbiota colonization process and in their metabolic response and intestinal functionalit...
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| Format: | Article |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85460-7 |
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| author | M. Saladrigas-García M. D’Angelo H. L. Ko S. Traserra P. Nolis Y. Ramayo-Caldas J. M. Folch P. Vergara P. Llonch J. F. Pérez S. M. Martín-Orúe |
| author_facet | M. Saladrigas-García M. D’Angelo H. L. Ko S. Traserra P. Nolis Y. Ramayo-Caldas J. M. Folch P. Vergara P. Llonch J. F. Pérez S. M. Martín-Orúe |
| author_sort | M. Saladrigas-García |
| collection | DOAJ |
| description | Abstract The aim of this study was to determine the possible impact of early socialization and an enriched neonatal environment to improve adaptation of piglets to weaning. We hypothesized that changes in the microbiota colonization process and in their metabolic response and intestinal functionality could help the animals face weaning stress. A total of 48 sows and their litters were allotted into a control (CTR) or an enriched treatment (ENR), in which piglets from two adjacent pens were combined and enriched with toys. The pattern of caecal microbial colonization, the jejunal gene expression, the serum metabolome and the intestinal physiology of the piglets were assessed before (-2 d) and after weaning (+ 3d). A differential ordination of caecal microbiota was observed after weaning. Serum metabolome suggested a reduced energetic metabolism in ENR animals, as evidenced by shifts in triglycerides and fatty acids, VLDL/LDL and creatine regions. The TLR2 gene showed to be downregulated in the jejunum of ENR pigs after weaning. The integration of gene expression, metabolome and microbiota datasets confirmed that differences between barren and enriched neonatal environments were evident only after weaning. Our results suggest that improvements in adaptation to weaning could be mediated by a better response to the post-weaning stress. |
| first_indexed | 2024-12-19T04:32:00Z |
| format | Article |
| id | doaj.art-e6eeec96253a407cb06139f75ebf725b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:32:00Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e6eeec96253a407cb06139f75ebf725b2022-12-21T20:35:51ZengNature PortfolioScientific Reports2045-23222021-03-0111111310.1038/s41598-021-85460-7Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaningM. Saladrigas-García0M. D’Angelo1H. L. Ko2S. Traserra3P. Nolis4Y. Ramayo-Caldas5J. M. Folch6P. Vergara7P. Llonch8J. F. Pérez9S. M. Martín-Orúe10Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona (UAB)Nuclear Magnetic Resonance (SeRMN), Universitat Autònoma de Barcelona (UAB)Animal Breeding and Genetics Program, Institute for Research and Technology in Food and Agriculture (IRTA)Plant and Animal Genomics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB ConsortiumDepartment of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona (UAB)Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Animal Nutrition and Welfare Service, Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB)Abstract The aim of this study was to determine the possible impact of early socialization and an enriched neonatal environment to improve adaptation of piglets to weaning. We hypothesized that changes in the microbiota colonization process and in their metabolic response and intestinal functionality could help the animals face weaning stress. A total of 48 sows and their litters were allotted into a control (CTR) or an enriched treatment (ENR), in which piglets from two adjacent pens were combined and enriched with toys. The pattern of caecal microbial colonization, the jejunal gene expression, the serum metabolome and the intestinal physiology of the piglets were assessed before (-2 d) and after weaning (+ 3d). A differential ordination of caecal microbiota was observed after weaning. Serum metabolome suggested a reduced energetic metabolism in ENR animals, as evidenced by shifts in triglycerides and fatty acids, VLDL/LDL and creatine regions. The TLR2 gene showed to be downregulated in the jejunum of ENR pigs after weaning. The integration of gene expression, metabolome and microbiota datasets confirmed that differences between barren and enriched neonatal environments were evident only after weaning. Our results suggest that improvements in adaptation to weaning could be mediated by a better response to the post-weaning stress.https://doi.org/10.1038/s41598-021-85460-7 |
| spellingShingle | M. Saladrigas-García M. D’Angelo H. L. Ko S. Traserra P. Nolis Y. Ramayo-Caldas J. M. Folch P. Vergara P. Llonch J. F. Pérez S. M. Martín-Orúe Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning Scientific Reports |
| title | Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| title_full | Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| title_fullStr | Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| title_full_unstemmed | Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| title_short | Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| title_sort | early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning |
| url | https://doi.org/10.1038/s41598-021-85460-7 |
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