NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i>
As one of the most imperative antioxidants in higher plants, carotenoids serve as accessory pigments to harvest light for photosynthesis as well as photoprotectors for plants to adapt to high light stress. Phytoene synthase (PSY) is the entry enzyme and also the major rate-limiting enzyme in the car...
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MDPI AG
2022-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/7/1134 |
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| author | Chen Dong Qingdong Wang Yubo Wang Lili Qin Yongchun Shi Xiaoran Wang Ran Wang |
| author_facet | Chen Dong Qingdong Wang Yubo Wang Lili Qin Yongchun Shi Xiaoran Wang Ran Wang |
| author_sort | Chen Dong |
| collection | DOAJ |
| description | As one of the most imperative antioxidants in higher plants, carotenoids serve as accessory pigments to harvest light for photosynthesis as well as photoprotectors for plants to adapt to high light stress. Phytoene synthase (PSY) is the entry enzyme and also the major rate-limiting enzyme in the carotenoid pathway. Here, we report a dehydration-responsive element-binding protein (DREB) transcription factor member in <i>Nicotiana tabacum</i> K326, NtDREB-1BL1, which regulates carotenoids biosynthesis by binding to the <i>NtPSY</i> promoter. The <i>NtDREB-1BL1</i> transcript was widely distributed in leaves by Real-time PCR. Confocal image revealed that NtDREB-1BL1 was localized in the nucleus. The chromatin immunoprecipitation (ChIP) with the qPCR technique indicated that NtDREB-1BL1 could anchor the promoter region of <i>NtPSY</i>. Overexpression (<i>NtDREB-1BL1 OE</i>) and RNA interference (<i>NtDREB-1BL1 RNAi</i>) of <i>NtDREB-1BL1</i> were performed to evaluate its biological function in <i>N. tabacum</i>. Both carotenoid and chlorophyll contents increased in transgenic plants of <i>NtDREB-1BL1 OE</i> compared with wild-type (WT) plants, with the augment of the genes involved in carotenoid biosynthesis. In contrast, the contents of carotenoid and chlorophyll significantly decreased in transgenic plants of <i>NtDREB-1BL1 RNAi</i> compared to WT, along with the decline in the expression of genes related to carotenoid biosynthesis. Moreover, transgenic plants of <i>NtDREB-1BL1 OE</i> exhibited enhanced tolerance under drought stress, with the weakened tolerance of drought stress in transgenic plants of <i>NtDREB-1BL1 RNAi</i>. In conclusion, our results illustrated the new role of transcription factor NtDREB-1BL1 in improving carotenoid biosynthesis through regulating <i>NtPSY</i> expression. |
| first_indexed | 2024-03-09T10:18:47Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:18:47Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-e6eef1c4b7ac45b4a400987ba52f0b8b2023-12-01T22:11:17ZengMDPI AGGenes2073-44252022-06-01137113410.3390/genes13071134NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i>Chen Dong0Qingdong Wang1Yubo Wang2Lili Qin3Yongchun Shi4Xiaoran Wang5Ran Wang6College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaAs one of the most imperative antioxidants in higher plants, carotenoids serve as accessory pigments to harvest light for photosynthesis as well as photoprotectors for plants to adapt to high light stress. Phytoene synthase (PSY) is the entry enzyme and also the major rate-limiting enzyme in the carotenoid pathway. Here, we report a dehydration-responsive element-binding protein (DREB) transcription factor member in <i>Nicotiana tabacum</i> K326, NtDREB-1BL1, which regulates carotenoids biosynthesis by binding to the <i>NtPSY</i> promoter. The <i>NtDREB-1BL1</i> transcript was widely distributed in leaves by Real-time PCR. Confocal image revealed that NtDREB-1BL1 was localized in the nucleus. The chromatin immunoprecipitation (ChIP) with the qPCR technique indicated that NtDREB-1BL1 could anchor the promoter region of <i>NtPSY</i>. Overexpression (<i>NtDREB-1BL1 OE</i>) and RNA interference (<i>NtDREB-1BL1 RNAi</i>) of <i>NtDREB-1BL1</i> were performed to evaluate its biological function in <i>N. tabacum</i>. Both carotenoid and chlorophyll contents increased in transgenic plants of <i>NtDREB-1BL1 OE</i> compared with wild-type (WT) plants, with the augment of the genes involved in carotenoid biosynthesis. In contrast, the contents of carotenoid and chlorophyll significantly decreased in transgenic plants of <i>NtDREB-1BL1 RNAi</i> compared to WT, along with the decline in the expression of genes related to carotenoid biosynthesis. Moreover, transgenic plants of <i>NtDREB-1BL1 OE</i> exhibited enhanced tolerance under drought stress, with the weakened tolerance of drought stress in transgenic plants of <i>NtDREB-1BL1 RNAi</i>. In conclusion, our results illustrated the new role of transcription factor NtDREB-1BL1 in improving carotenoid biosynthesis through regulating <i>NtPSY</i> expression.https://www.mdpi.com/2073-4425/13/7/1134antioxidantscarotenoid biosynthesisDREBdrought stressphytoene synthasetranscription factor |
| spellingShingle | Chen Dong Qingdong Wang Yubo Wang Lili Qin Yongchun Shi Xiaoran Wang Ran Wang NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> Genes antioxidants carotenoid biosynthesis DREB drought stress phytoene synthase transcription factor |
| title | NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> |
| title_full | NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> |
| title_fullStr | NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> |
| title_full_unstemmed | NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> |
| title_short | NtDREB-1BL1 Enhances Carotenoid Biosynthesis by Regulating Phytoene Synthase in <i>Nicotiana tabacum</i> |
| title_sort | ntdreb 1bl1 enhances carotenoid biosynthesis by regulating phytoene synthase in i nicotiana tabacum i |
| topic | antioxidants carotenoid biosynthesis DREB drought stress phytoene synthase transcription factor |
| url | https://www.mdpi.com/2073-4425/13/7/1134 |
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