Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction
Introduction. Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. Objective. Therefore, the goal of this study...
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| Format: | Article |
| Language: | English |
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Serbian Medical Society
2014-01-01
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| Series: | Srpski Arhiv za Celokupno Lekarstvo |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791412708M.pdf |
| _version_ | 1819051596380635136 |
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| author | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Matkić Aleksandra Ristić Ljiljana |
| author_facet | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Matkić Aleksandra Ristić Ljiljana |
| author_sort | Miljković-Selimović Biljana |
| collection | DOAJ |
| description | Introduction. Campylobacter jejuni (C. jejuni) and Campylobacter coli (C.
coli) are the most common bacterial causes of enterocolitis in humans.
However, identification of the species level is not always possible using
standard biochemical tests. Objective. Therefore, the goal of this study was
to identify these microorganisms by both phenotyping and polymerase chain
reaction (PCR) technique. Methods. A total of 153 species of thermophilic
campylobacters were examined with standard biochemical tests and PCR
technique to prove hipO genes of C. jejuni and asp genes of C. coli. Results
Standard biochemical tests enabled the speciation of 121 strains of C.
jejuni, while application of PCR detected 126 C. jejuni strains. Conclusion.
PCR technique allowed not only identification of hippurate-positive C.
jejuni, but also hipuratnegative strains of C. jejuni which otherwise would
be detected as C. coli if only biochemical tests were applied. |
| first_indexed | 2024-12-21T12:06:27Z |
| format | Article |
| id | doaj.art-e6f154d61d6e4ebfbbf7a700ccd59b33 |
| institution | Directory Open Access Journal |
| issn | 0370-8179 |
| language | English |
| last_indexed | 2024-12-21T12:06:27Z |
| publishDate | 2014-01-01 |
| publisher | Serbian Medical Society |
| record_format | Article |
| series | Srpski Arhiv za Celokupno Lekarstvo |
| spelling | doaj.art-e6f154d61d6e4ebfbbf7a700ccd59b332022-12-21T19:04:42ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792014-01-0114211-1270871210.2298/SARH1412708M0370-81791412708MIdentification of campylobacter species isolates with phenotypic methods and polymerase chain reactionMiljković-Selimović Biljana0Babić Tatjana1Kocić Branislava2Matkić Aleksandra3Ristić Ljiljana4School of Medicine, Niš + Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, NišInstitute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, NišSchool of Medicine, Niš + Institute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, NišSchool of Medicine, NišInstitute for Public Health, National Referent Laboratory for Campylobacter and Helicobacter, Center for Microbiology, NišIntroduction. Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. Objective. Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. Methods. A total of 153 species of thermophilic campylobacters were examined with standard biochemical tests and PCR technique to prove hipO genes of C. jejuni and asp genes of C. coli. Results Standard biochemical tests enabled the speciation of 121 strains of C. jejuni, while application of PCR detected 126 C. jejuni strains. Conclusion. PCR technique allowed not only identification of hippurate-positive C. jejuni, but also hipuratnegative strains of C. jejuni which otherwise would be detected as C. coli if only biochemical tests were applied.http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791412708M.pdfCampylobacter jejuniCampylobacter coliidentificationPCR |
| spellingShingle | Miljković-Selimović Biljana Babić Tatjana Kocić Branislava Matkić Aleksandra Ristić Ljiljana Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction Srpski Arhiv za Celokupno Lekarstvo Campylobacter jejuni Campylobacter coli identification PCR |
| title | Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| title_full | Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| title_fullStr | Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| title_full_unstemmed | Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| title_short | Identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| title_sort | identification of campylobacter species isolates with phenotypic methods and polymerase chain reaction |
| topic | Campylobacter jejuni Campylobacter coli identification PCR |
| url | http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791412708M.pdf |
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