Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ
Effective amelioration of type II diabetes requires therapies that increase both glucose uptake activity per cell and skeletal muscle mass. Myristic acid (14:0) increases diacylglycerol kinase (DGK) δ protein levels and enhances glucose uptake in myotubes in a DGKδ‐dependent manner. However, it is s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13466 |
| _version_ | 1811181706223288320 |
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| author | Hiromichi Sakai Ken‐ichi Matsumoto Takeshi Urano Fumio Sakane |
| author_facet | Hiromichi Sakai Ken‐ichi Matsumoto Takeshi Urano Fumio Sakane |
| author_sort | Hiromichi Sakai |
| collection | DOAJ |
| description | Effective amelioration of type II diabetes requires therapies that increase both glucose uptake activity per cell and skeletal muscle mass. Myristic acid (14:0) increases diacylglycerol kinase (DGK) δ protein levels and enhances glucose uptake in myotubes in a DGKδ‐dependent manner. However, it is still unclear whether myristic acid treatment affects skeletal muscle mass. In this study, we found that myristic acid treatment increased the protein level of β‐tubulin, which constitutes microtubules and is closely related to muscle mass, in C2C12 myotubes but not in the proliferation stage in C2C12 myoblasts. However, lauric (12:0), palmitic (16:0) and oleic (18:1) acids failed to affect DGKδ and β‐tubulin protein levels in C2C12 myotubes. Moreover, knockdown of DGKδ by siRNA significantly inhibited the increased protein level of β‐tubulin in the presence of myristic acid, suggesting that the increase in β‐tubulin protein by myristic acid depends on DGKδ. These results indicate that myristic acid selectively affects β‐tubulin protein levels in C2C12 myotubes via DGKδ, suggesting that this fatty acid improves skeletal muscle mass in addition to increasing glucose uptake activity per cell. |
| first_indexed | 2024-04-11T09:21:56Z |
| format | Article |
| id | doaj.art-e6f3b3f04757499c8a826dd27186b038 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T09:21:56Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-e6f3b3f04757499c8a826dd27186b0382022-12-22T04:32:10ZengWileyFEBS Open Bio2211-54632022-10-0112101788179610.1002/2211-5463.13466Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δHiromichi Sakai0Ken‐ichi Matsumoto1Takeshi Urano2Fumio Sakane3Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research and Academic Information Shimane University Izumo JapanDepartment of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research and Academic Information Shimane University Izumo JapanDepartment of Biochemistry Shimane University School of Medicine Izumo JapanDepartment of Chemistry, Graduate School of Science Chiba University JapanEffective amelioration of type II diabetes requires therapies that increase both glucose uptake activity per cell and skeletal muscle mass. Myristic acid (14:0) increases diacylglycerol kinase (DGK) δ protein levels and enhances glucose uptake in myotubes in a DGKδ‐dependent manner. However, it is still unclear whether myristic acid treatment affects skeletal muscle mass. In this study, we found that myristic acid treatment increased the protein level of β‐tubulin, which constitutes microtubules and is closely related to muscle mass, in C2C12 myotubes but not in the proliferation stage in C2C12 myoblasts. However, lauric (12:0), palmitic (16:0) and oleic (18:1) acids failed to affect DGKδ and β‐tubulin protein levels in C2C12 myotubes. Moreover, knockdown of DGKδ by siRNA significantly inhibited the increased protein level of β‐tubulin in the presence of myristic acid, suggesting that the increase in β‐tubulin protein by myristic acid depends on DGKδ. These results indicate that myristic acid selectively affects β‐tubulin protein levels in C2C12 myotubes via DGKδ, suggesting that this fatty acid improves skeletal muscle mass in addition to increasing glucose uptake activity per cell.https://doi.org/10.1002/2211-5463.13466diacylglycerol kinasemyotubemyristic acidtype II diabetesβ‐tubulin |
| spellingShingle | Hiromichi Sakai Ken‐ichi Matsumoto Takeshi Urano Fumio Sakane Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ FEBS Open Bio diacylglycerol kinase myotube myristic acid type II diabetes β‐tubulin |
| title | Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ |
| title_full | Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ |
| title_fullStr | Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ |
| title_full_unstemmed | Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ |
| title_short | Myristic acid selectively augments β‐tubulin levels in C2C12 myotubes via diacylglycerol kinase δ |
| title_sort | myristic acid selectively augments β tubulin levels in c2c12 myotubes via diacylglycerol kinase δ |
| topic | diacylglycerol kinase myotube myristic acid type II diabetes β‐tubulin |
| url | https://doi.org/10.1002/2211-5463.13466 |
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