Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition
Background: Increasing evidence points to the esophageal microbiome as an important co-factor in esophageal neoplasia. Esophageal microbiome composition is strongly influenced by the oral microbiome. Salivary microbiome assessment has emerged as a potential non-invasive tool to identify patients at...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/8/1739 |
| _version_ | 1797522812636758016 |
|---|---|
| author | Judith Kim Minyi Lee Brittany Baldwin-Hunter Quinn S. Solfisburg Charles J. Lightdale Tal Korem Chin Hur Julian A. Abrams |
| author_facet | Judith Kim Minyi Lee Brittany Baldwin-Hunter Quinn S. Solfisburg Charles J. Lightdale Tal Korem Chin Hur Julian A. Abrams |
| author_sort | Judith Kim |
| collection | DOAJ |
| description | Background: Increasing evidence points to the esophageal microbiome as an important co-factor in esophageal neoplasia. Esophageal microbiome composition is strongly influenced by the oral microbiome. Salivary microbiome assessment has emerged as a potential non-invasive tool to identify patients at risk for esophageal cancer, but key host and environmental factors that may affect the salivary microbiome have not been well-defined. This study aimed to evaluate the impact of short-term dietary intake on salivary microbiome composition. Methods: Saliva samples were collected from 69 subjects prior to upper endoscopy who completed the Automated Self-Administered 24-Hour (ASA24) Dietary Assessment. Salivary microbiome composition was determined using 16S rRNA amplicon sequencing. Results: There was no significant correlation between alpha diversity and primary measures of short-term dietary intake (total daily calories, fat, fiber, fruit/vegetables, red meat intake, and fasting time). There was no evidence of clustering on beta diversity analyses. Very few taxonomic alterations were found for short-term dietary intake; an increased relative abundance of <i>Neisseria oralis</i> and <i>Lautropia sp.</i> was associated with high fruit and vegetable intake, and an increased relative abundance of a taxon in the family <i>Gemellaceae</i> was associated with increased red meat intake. Conclusions: Short-term dietary intake was associated with only minimal salivary microbiome alterations and does not appear to have a major impact on the potential use of the salivary microbiome as a biomarker for esophageal neoplasia. |
| first_indexed | 2024-03-10T08:34:39Z |
| format | Article |
| id | doaj.art-e6f73dd46cf54be7b73063b81dbe0b0e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:39Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e6f73dd46cf54be7b73063b81dbe0b0e2023-11-22T08:48:11ZengMDPI AGMicroorganisms2076-26072021-08-0198173910.3390/microorganisms9081739Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome CompositionJudith Kim0Minyi Lee1Brittany Baldwin-Hunter2Quinn S. Solfisburg3Charles J. Lightdale4Tal Korem5Chin Hur6Julian A. Abrams7Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USASchool of Medicine, Boston University, Boston, MA 02118, USADepartment of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USAVagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USADepartment of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USAProgram for Mathematical Genomics, Department of Systems Biology, Columbia University, New York, NY 10032, USADepartment of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USADepartment of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USABackground: Increasing evidence points to the esophageal microbiome as an important co-factor in esophageal neoplasia. Esophageal microbiome composition is strongly influenced by the oral microbiome. Salivary microbiome assessment has emerged as a potential non-invasive tool to identify patients at risk for esophageal cancer, but key host and environmental factors that may affect the salivary microbiome have not been well-defined. This study aimed to evaluate the impact of short-term dietary intake on salivary microbiome composition. Methods: Saliva samples were collected from 69 subjects prior to upper endoscopy who completed the Automated Self-Administered 24-Hour (ASA24) Dietary Assessment. Salivary microbiome composition was determined using 16S rRNA amplicon sequencing. Results: There was no significant correlation between alpha diversity and primary measures of short-term dietary intake (total daily calories, fat, fiber, fruit/vegetables, red meat intake, and fasting time). There was no evidence of clustering on beta diversity analyses. Very few taxonomic alterations were found for short-term dietary intake; an increased relative abundance of <i>Neisseria oralis</i> and <i>Lautropia sp.</i> was associated with high fruit and vegetable intake, and an increased relative abundance of a taxon in the family <i>Gemellaceae</i> was associated with increased red meat intake. Conclusions: Short-term dietary intake was associated with only minimal salivary microbiome alterations and does not appear to have a major impact on the potential use of the salivary microbiome as a biomarker for esophageal neoplasia.https://www.mdpi.com/2076-2607/9/8/1739salivary microbiomeesophageal cancerdietary intakeesophageal microbiome |
| spellingShingle | Judith Kim Minyi Lee Brittany Baldwin-Hunter Quinn S. Solfisburg Charles J. Lightdale Tal Korem Chin Hur Julian A. Abrams Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition Microorganisms salivary microbiome esophageal cancer dietary intake esophageal microbiome |
| title | Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition |
| title_full | Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition |
| title_fullStr | Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition |
| title_full_unstemmed | Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition |
| title_short | Minimal Associations between Short-Term Dietary Intake and Salivary Microbiome Composition |
| title_sort | minimal associations between short term dietary intake and salivary microbiome composition |
| topic | salivary microbiome esophageal cancer dietary intake esophageal microbiome |
| url | https://www.mdpi.com/2076-2607/9/8/1739 |
| work_keys_str_mv | AT judithkim minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT minyilee minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT brittanybaldwinhunter minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT quinnssolfisburg minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT charlesjlightdale minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT talkorem minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT chinhur minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition AT julianaabrams minimalassociationsbetweenshorttermdietaryintakeandsalivarymicrobiomecomposition |