Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice
CpG-oligodeoxynucleotides (CpG-ODNs) constitute an attractive alternative for asthma treatment. However, very little evidence is available from studies on the oral administration of CpG-ODNs in animals. Previously, we developed acid-resistant particles (named ODNcap) as an oral delivery device for O...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.738041/full |
| _version_ | 1819001101272219648 |
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| author | Takuma Okajima Suguru Shigemori Fu Namai Tasuku Ogita Takashi Sato Takeshi Shimosato |
| author_facet | Takuma Okajima Suguru Shigemori Fu Namai Tasuku Ogita Takashi Sato Takeshi Shimosato |
| author_sort | Takuma Okajima |
| collection | DOAJ |
| description | CpG-oligodeoxynucleotides (CpG-ODNs) constitute an attractive alternative for asthma treatment. However, very little evidence is available from studies on the oral administration of CpG-ODNs in animals. Previously, we developed acid-resistant particles (named ODNcap) as an oral delivery device for ODNs. Here, we showed that free feeding of an ODNcap-containing feed prophylactically attenuates allergic airway inflammation, hyperresponsiveness, and goblet cell hyperplasia in an ovalbumin-induced asthma model. Using transcriptomics-driven approaches, we demonstrated that injury of pulmonary vein cardiomyocytes accompanies allergen inhalation challenge, but is inhibited by ODNcap feeding. We also showed the participation of an airway antimicrobial peptide (Reg3γ) and fecal microbiota in the ODNcap-mediated effects. Collectively, our findings suggest that daily oral ingestion of ODNcap may provide preventive effects on allergic bronchopulmonary insults via regulation of mechanisms involved in the gut-lung connection. |
| first_indexed | 2024-12-20T22:43:51Z |
| format | Article |
| id | doaj.art-e6f9232b12ee4526a10083ad0fa1845b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T22:43:51Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e6f9232b12ee4526a10083ad0fa1845b2022-12-21T19:24:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-11-011210.3389/fimmu.2021.738041738041Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in MiceTakuma OkajimaSuguru ShigemoriFu NamaiTasuku OgitaTakashi SatoTakeshi ShimosatoCpG-oligodeoxynucleotides (CpG-ODNs) constitute an attractive alternative for asthma treatment. However, very little evidence is available from studies on the oral administration of CpG-ODNs in animals. Previously, we developed acid-resistant particles (named ODNcap) as an oral delivery device for ODNs. Here, we showed that free feeding of an ODNcap-containing feed prophylactically attenuates allergic airway inflammation, hyperresponsiveness, and goblet cell hyperplasia in an ovalbumin-induced asthma model. Using transcriptomics-driven approaches, we demonstrated that injury of pulmonary vein cardiomyocytes accompanies allergen inhalation challenge, but is inhibited by ODNcap feeding. We also showed the participation of an airway antimicrobial peptide (Reg3γ) and fecal microbiota in the ODNcap-mediated effects. Collectively, our findings suggest that daily oral ingestion of ODNcap may provide preventive effects on allergic bronchopulmonary insults via regulation of mechanisms involved in the gut-lung connection.https://www.frontiersin.org/articles/10.3389/fimmu.2021.738041/fullCpG-ODNsoral deliverymouse model of allergic asthmaairway inflammationairway hyperresponsivenesspulmonary vein cardiomyocytes |
| spellingShingle | Takuma Okajima Suguru Shigemori Fu Namai Tasuku Ogita Takashi Sato Takeshi Shimosato Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice Frontiers in Immunology CpG-ODNs oral delivery mouse model of allergic asthma airway inflammation airway hyperresponsiveness pulmonary vein cardiomyocytes |
| title | Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice |
| title_full | Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice |
| title_fullStr | Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice |
| title_full_unstemmed | Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice |
| title_short | Free Feeding of CpG-Oligodeoxynucleotide Particles Prophylactically Attenuates Allergic Airway Inflammation and Hyperresponsiveness in Mice |
| title_sort | free feeding of cpg oligodeoxynucleotide particles prophylactically attenuates allergic airway inflammation and hyperresponsiveness in mice |
| topic | CpG-ODNs oral delivery mouse model of allergic asthma airway inflammation airway hyperresponsiveness pulmonary vein cardiomyocytes |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.738041/full |
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