Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories
Abstract Valencene is a natural sesquiterpene with desirable bioactivity and aroma, making it a valuable ingredient in the food and cosmetics industries. Traditionally, valencene was extracted from the citrus fruits, and its applications were restricted by the low concentrations in natural sources a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | GCB Bioenergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/gcbb.13086 |
| _version_ | 1797743819910807552 |
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| author | Jiahui Sun Xuejing Xu Yannan Wu Huili Sun Guodong Luan Xuefeng Lu |
| author_facet | Jiahui Sun Xuejing Xu Yannan Wu Huili Sun Guodong Luan Xuefeng Lu |
| author_sort | Jiahui Sun |
| collection | DOAJ |
| description | Abstract Valencene is a natural sesquiterpene with desirable bioactivity and aroma, making it a valuable ingredient in the food and cosmetics industries. Traditionally, valencene was extracted from the citrus fruits, and its applications were restricted by the low concentrations in natural sources and high costs for extraction. Photosynthetic biomanufacturing represents a promising route for efficient and stable production of valencene, while cyanobacteria have been considered one of the most promising platforms regarding biotechnological routes for the direct conversion of CO2. In this work, we engineered Synechocystis sp. PCC 6803 to synthesize valencene. By introducing a heterologous valencene synthase and modifying the native MEP pathway, we obtained an efficient cyanobacterial cell factory that produced 154 mg/L valencene during a semi‐continual cultivation, with an average productivity of 4.3 mg/L/day, and the cell factory exhibited robust growth and production in non‐sterilized conditions. We also achieved the production of other sesquiterpenes including bisabolene, amorpha‐4,11‐diene, farnesene, and nerolidol by engineered cyanobacteria with enhanced MEP pathway flux, showing promising potentials as a universal chassis. |
| first_indexed | 2024-03-12T15:00:57Z |
| format | Article |
| id | doaj.art-e6f9e300b2224da0a4efa8d1f544025c |
| institution | Directory Open Access Journal |
| issn | 1757-1693 1757-1707 |
| language | English |
| last_indexed | 2024-03-12T15:00:57Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj.art-e6f9e300b2224da0a4efa8d1f544025c2023-08-14T08:10:17ZengWileyGCB Bioenergy1757-16931757-17072023-09-011591154116510.1111/gcbb.13086Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factoriesJiahui Sun0Xuejing Xu1Yannan Wu2Huili Sun3Guodong Luan4Xuefeng Lu5Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao ChinaAbstract Valencene is a natural sesquiterpene with desirable bioactivity and aroma, making it a valuable ingredient in the food and cosmetics industries. Traditionally, valencene was extracted from the citrus fruits, and its applications were restricted by the low concentrations in natural sources and high costs for extraction. Photosynthetic biomanufacturing represents a promising route for efficient and stable production of valencene, while cyanobacteria have been considered one of the most promising platforms regarding biotechnological routes for the direct conversion of CO2. In this work, we engineered Synechocystis sp. PCC 6803 to synthesize valencene. By introducing a heterologous valencene synthase and modifying the native MEP pathway, we obtained an efficient cyanobacterial cell factory that produced 154 mg/L valencene during a semi‐continual cultivation, with an average productivity of 4.3 mg/L/day, and the cell factory exhibited robust growth and production in non‐sterilized conditions. We also achieved the production of other sesquiterpenes including bisabolene, amorpha‐4,11‐diene, farnesene, and nerolidol by engineered cyanobacteria with enhanced MEP pathway flux, showing promising potentials as a universal chassis.https://doi.org/10.1111/gcbb.13086cyanobacteriametabolic engineeringphotosynthesissesquiterpenevalencene |
| spellingShingle | Jiahui Sun Xuejing Xu Yannan Wu Huili Sun Guodong Luan Xuefeng Lu Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories GCB Bioenergy cyanobacteria metabolic engineering photosynthesis sesquiterpene valencene |
| title | Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories |
| title_full | Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories |
| title_fullStr | Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories |
| title_full_unstemmed | Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories |
| title_short | Conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered Synechocystis sp. PCC 6803 cell factories |
| title_sort | conversion of carbon dioxide into valencene and other sesquiterpenes with metabolic engineered synechocystis sp pcc 6803 cell factories |
| topic | cyanobacteria metabolic engineering photosynthesis sesquiterpene valencene |
| url | https://doi.org/10.1111/gcbb.13086 |
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