Serological Identification of Virus in Watermelon Production Fields in the Tocantins State
Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the oc...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-04-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192&lng=en&tlng=en |
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| author | Raimundo Wagner de Souza Aguiar Adelmo Rodrigues Augustus Caeser Franke Portella Magnolia de Mendonça Lopes Mirtes Freitas Lima Renato de Oliveira Resende Tatsuya Nagata |
| author_facet | Raimundo Wagner de Souza Aguiar Adelmo Rodrigues Augustus Caeser Franke Portella Magnolia de Mendonça Lopes Mirtes Freitas Lima Renato de Oliveira Resende Tatsuya Nagata |
| author_sort | Raimundo Wagner de Souza Aguiar |
| collection | DOAJ |
| description | Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of viruses, such as Papaya ringspot W (PRSV-W), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV) (potyvirus), Cucumber mosaic virus ( CMV) (Cucumovirus) and Zucchini lethal chlorosis virus (ZLCV) (Tospovirus). Of these, PRSV-W was predominant (22%), followed by WMV (15%), ZLCV (11%), CMV (5%) and ZYMV (4%). Mixed infections with PRSV-W + WMV and PRSV-W + ZLCV were also observed around 20% frequency (expressed with symptoms differently from a single infection). The results provide important support for the program management viruses. |
| first_indexed | 2024-04-13T22:39:30Z |
| format | Article |
| id | doaj.art-e6fa271392ca48ed93ae1ead709f717c |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-13T22:39:30Z |
| publishDate | 2015-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-e6fa271392ca48ed93ae1ead709f717c2022-12-22T02:26:40ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-04-0158219219710.1590/S1516-8913201400219S1516-89132015000200192Serological Identification of Virus in Watermelon Production Fields in the Tocantins StateRaimundo Wagner de Souza AguiarAdelmo RodriguesAugustus Caeser Franke PortellaMagnolia de Mendonça LopesMirtes Freitas LimaRenato de Oliveira ResendeTatsuya NagataWatermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of viruses, such as Papaya ringspot W (PRSV-W), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV) (potyvirus), Cucumber mosaic virus ( CMV) (Cucumovirus) and Zucchini lethal chlorosis virus (ZLCV) (Tospovirus). Of these, PRSV-W was predominant (22%), followed by WMV (15%), ZLCV (11%), CMV (5%) and ZYMV (4%). Mixed infections with PRSV-W + WMV and PRSV-W + ZLCV were also observed around 20% frequency (expressed with symptoms differently from a single infection). The results provide important support for the program management viruses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192&lng=en&tlng=enWatermelonCitrullus lanatuspotyviruscucumovirustospovirusserological assay |
| spellingShingle | Raimundo Wagner de Souza Aguiar Adelmo Rodrigues Augustus Caeser Franke Portella Magnolia de Mendonça Lopes Mirtes Freitas Lima Renato de Oliveira Resende Tatsuya Nagata Serological Identification of Virus in Watermelon Production Fields in the Tocantins State Brazilian Archives of Biology and Technology Watermelon Citrullus lanatus potyvirus cucumovirus tospovirus serological assay |
| title | Serological Identification of Virus in Watermelon Production Fields in the Tocantins State |
| title_full | Serological Identification of Virus in Watermelon Production Fields in the Tocantins State |
| title_fullStr | Serological Identification of Virus in Watermelon Production Fields in the Tocantins State |
| title_full_unstemmed | Serological Identification of Virus in Watermelon Production Fields in the Tocantins State |
| title_short | Serological Identification of Virus in Watermelon Production Fields in the Tocantins State |
| title_sort | serological identification of virus in watermelon production fields in the tocantins state |
| topic | Watermelon Citrullus lanatus potyvirus cucumovirus tospovirus serological assay |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192&lng=en&tlng=en |
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