Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls
ABSTRACTBackground Infective endocarditis (IE) is an uncommon disease with high morbidity and mortality rates, which often develops from oral bacterial species entering circulation.Objective We compared oral microbiome profiles of three groups: IE patients (N 9 patients; n = 27 samples), disease co...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2022.2144614 |
| _version_ | 1797449994824843264 |
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| author | Jean-Luc C. Mougeot Micaela Beckman Bruce J. Paster Peter B. Lockhart Farah Bahrani Mougeot |
| author_facet | Jean-Luc C. Mougeot Micaela Beckman Bruce J. Paster Peter B. Lockhart Farah Bahrani Mougeot |
| author_sort | Jean-Luc C. Mougeot |
| collection | DOAJ |
| description | ABSTRACTBackground Infective endocarditis (IE) is an uncommon disease with high morbidity and mortality rates, which often develops from oral bacterial species entering circulation.Objective We compared oral microbiome profiles of three groups: IE patients (N 9 patients; n = 27 samples), disease controls at risk for IE (N = 28; n = 84), and healthy controls (N = 37; n = 111). Bacterial species in IE patients’ blood cultures were identified for comparison with matched oral samples.Design Oral microbiome profiles were obtained from buccal mucosa, saliva, and tongue samples for all three groups and from sub- and supra-gingival plaque samples of the IE group (N = 9; n = 16) and disease controls (N = 28; n = 54). Alpha- and beta-diversities were determined based on relative abundance data. Discriminative species were identified by LEfSe, post hoc Mann-Whitney, and ROC analyses. Identity of the bacterial species in IE patients’ blood cultures was confirmed by 16S-rRNA gene Sanger sequencing.Results Alpha- and beta-diversities differed between groups. Discriminative IE-associated species were identified, e.g. Haemophilus parainfluenzae and Streptococcus sanguinis. Two blood isolates were Staphylococcus aureus, also identified in one matched saliva sample. Streptococcus mutans was present in one patient’s plaque samples and blood culture.Conclusions Oral microbiomes of IE, non-IE disease controls, and healthy controls differed significantly. A better understanding of IE-related bacterial-host interactions is warranted. |
| first_indexed | 2024-03-09T14:33:09Z |
| format | Article |
| id | doaj.art-e6fd5931cc6843f09cd3a1094db32719 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:33:09Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-e6fd5931cc6843f09cd3a1094db327192023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2022.2144614Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controlsJean-Luc C. Mougeot0Micaela Beckman1Bruce J. Paster2Peter B. Lockhart3Farah Bahrani Mougeot4Translational Research Laboratory, Department of Oral Medicine/ Oral Maxillofacial Surgery, Cannon Research Center, Carolinas Medical Center, Atrium Heath, Charlotte, NC, USATranslational Research Laboratory, Department of Oral Medicine/ Oral Maxillofacial Surgery, Cannon Research Center, Carolinas Medical Center, Atrium Heath, Charlotte, NC, USADepartment of Microbiology, the Forsyth Institute, Cambridge, MA, USATranslational Research Laboratory, Department of Oral Medicine/ Oral Maxillofacial Surgery, Cannon Research Center, Carolinas Medical Center, Atrium Heath, Charlotte, NC, USATranslational Research Laboratory, Department of Oral Medicine/ Oral Maxillofacial Surgery, Cannon Research Center, Carolinas Medical Center, Atrium Heath, Charlotte, NC, USAABSTRACTBackground Infective endocarditis (IE) is an uncommon disease with high morbidity and mortality rates, which often develops from oral bacterial species entering circulation.Objective We compared oral microbiome profiles of three groups: IE patients (N 9 patients; n = 27 samples), disease controls at risk for IE (N = 28; n = 84), and healthy controls (N = 37; n = 111). Bacterial species in IE patients’ blood cultures were identified for comparison with matched oral samples.Design Oral microbiome profiles were obtained from buccal mucosa, saliva, and tongue samples for all three groups and from sub- and supra-gingival plaque samples of the IE group (N = 9; n = 16) and disease controls (N = 28; n = 54). Alpha- and beta-diversities were determined based on relative abundance data. Discriminative species were identified by LEfSe, post hoc Mann-Whitney, and ROC analyses. Identity of the bacterial species in IE patients’ blood cultures was confirmed by 16S-rRNA gene Sanger sequencing.Results Alpha- and beta-diversities differed between groups. Discriminative IE-associated species were identified, e.g. Haemophilus parainfluenzae and Streptococcus sanguinis. Two blood isolates were Staphylococcus aureus, also identified in one matched saliva sample. Streptococcus mutans was present in one patient’s plaque samples and blood culture.Conclusions Oral microbiomes of IE, non-IE disease controls, and healthy controls differed significantly. A better understanding of IE-related bacterial-host interactions is warranted.https://www.tandfonline.com/doi/10.1080/20002297.2022.2144614Infective endocarditisoral microbiomenext-generation sequencingblood culturebacterial species |
| spellingShingle | Jean-Luc C. Mougeot Micaela Beckman Bruce J. Paster Peter B. Lockhart Farah Bahrani Mougeot Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls Journal of Oral Microbiology Infective endocarditis oral microbiome next-generation sequencing blood culture bacterial species |
| title | Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls |
| title_full | Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls |
| title_fullStr | Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls |
| title_full_unstemmed | Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls |
| title_short | Oral microbiomes of patients with infective endocarditis (IE): a comparative pilot study of IE patients, patients at risk for IE and healthy controls |
| title_sort | oral microbiomes of patients with infective endocarditis ie a comparative pilot study of ie patients patients at risk for ie and healthy controls |
| topic | Infective endocarditis oral microbiome next-generation sequencing blood culture bacterial species |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2022.2144614 |
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