Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-06-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/7/1535 |
| _version_ | 1818607397928697856 |
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| author | Kristof Harda Zsuzsanna Szabo Erzsebet Szabo Gabor Olah Klara Fodor Csaba Szasz Gabor Mehes Andrew V. Schally Gabor Halmos |
| author_facet | Kristof Harda Zsuzsanna Szabo Erzsebet Szabo Gabor Olah Klara Fodor Csaba Szasz Gabor Mehes Andrew V. Schally Gabor Halmos |
| author_sort | Kristof Harda |
| collection | DOAJ |
| description | Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors. |
| first_indexed | 2024-12-16T14:26:07Z |
| format | Article |
| id | doaj.art-e6fe89552388451195697b29533676cb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-16T14:26:07Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e6fe89552388451195697b29533676cb2022-12-21T22:28:23ZengMDPI AGMolecules1420-30492018-06-01237153510.3390/molecules23071535molecules23071535Somatostatin Receptors as Molecular Targets in Human Uveal MelanomaKristof Harda0Zsuzsanna Szabo1Erzsebet Szabo2Gabor Olah3Klara Fodor4Csaba Szasz5Gabor Mehes6Andrew V. Schally7Gabor Halmos8Department of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pathology, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pathology, University of Debrecen, 4032 Debrecen, HungaryEndocrine, Polypeptide and Cancer Institute, Veterans Affairs Medical Center, Miami, FL 33125, USADepartment of Biopharmacy, University of Debrecen, 4032 Debrecen, HungaryUveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4–5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1–5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.http://www.mdpi.com/1420-3049/23/7/1535human uveal melanomasomatostatin receptorsmolecular targets SSTR-1-5cancer therapy |
| spellingShingle | Kristof Harda Zsuzsanna Szabo Erzsebet Szabo Gabor Olah Klara Fodor Csaba Szasz Gabor Mehes Andrew V. Schally Gabor Halmos Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma Molecules human uveal melanoma somatostatin receptors molecular targets SSTR-1-5 cancer therapy |
| title | Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma |
| title_full | Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma |
| title_fullStr | Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma |
| title_full_unstemmed | Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma |
| title_short | Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma |
| title_sort | somatostatin receptors as molecular targets in human uveal melanoma |
| topic | human uveal melanoma somatostatin receptors molecular targets SSTR-1-5 cancer therapy |
| url | http://www.mdpi.com/1420-3049/23/7/1535 |
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