Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos
Germ cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, piRNAs in mouse oocytes are relatively rare and dispensable. We present compelling evidence for strong Piwi and piRNA expression in oocytes of other mammals. Human fetal oocytes express PIWIL2 and transposon-enri...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-03-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715002399 |
| _version_ | 1811227813972279296 |
|---|---|
| author | Elke F. Roovers David Rosenkranz Mahdi Mahdipour Chung-Ting Han Nannan He Susana M. Chuva de Sousa Lopes Lucette A.J. van der Westerlaken Hans Zischler Falk Butter Bernard A.J. Roelen René F. Ketting |
| author_facet | Elke F. Roovers David Rosenkranz Mahdi Mahdipour Chung-Ting Han Nannan He Susana M. Chuva de Sousa Lopes Lucette A.J. van der Westerlaken Hans Zischler Falk Butter Bernard A.J. Roelen René F. Ketting |
| author_sort | Elke F. Roovers |
| collection | DOAJ |
| description | Germ cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, piRNAs in mouse oocytes are relatively rare and dispensable. We present compelling evidence for strong Piwi and piRNA expression in oocytes of other mammals. Human fetal oocytes express PIWIL2 and transposon-enriched piRNAs. Oocytes in adult human ovary express PIWIL1 and PIWIL2, whereas those in bovine ovary only express PIWIL1. In human, macaque, and bovine ovaries, we find piRNAs that resemble testis-borne pachytene piRNAs. Isolated bovine follicular oocytes were shown to contain abundant, relatively short piRNAs that preferentially target transposable elements. Using label-free quantitative proteome analysis, we show that these maturing oocytes strongly and specifically express the PIWIL3 protein, alongside other, known piRNA-pathway components. A piRNA pool is still present in early bovine embryos, revealing a potential impact of piRNAs on mammalian embryogenesis. Our results reveal that there are highly dynamic piRNA pathways in mammalian oocytes and early embryos. |
| first_indexed | 2024-04-12T09:47:04Z |
| format | Article |
| id | doaj.art-e6ff335ba2ba4aeca2b0ee808d99219a |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T09:47:04Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e6ff335ba2ba4aeca2b0ee808d99219a2022-12-22T03:37:55ZengElsevierCell Reports2211-12472015-03-0110122069208210.1016/j.celrep.2015.02.062Piwi Proteins and piRNAs in Mammalian Oocytes and Early EmbryosElke F. Roovers0David Rosenkranz1Mahdi Mahdipour2Chung-Ting Han3Nannan He4Susana M. Chuva de Sousa Lopes5Lucette A.J. van der Westerlaken6Hans Zischler7Falk Butter8Bernard A.J. Roelen9René F. Ketting10Biology of Non-coding RNA Group, Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, GermanyJohannes Gutenberg-University Mainz, Institute of Anthropology, Anselm-Franz-von-Bentzel-Weg 7, 55128 Mainz, GermanyDepartment of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the NetherlandsGenomics Core Facility, Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, GermanyDepartment of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZA Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZA Leiden, the NetherlandsDepartment of Gynaecology, Leiden University Medical Center, Albinusdreef 2, 2300RC Leiden, the NetherlandsJohannes Gutenberg-University Mainz, Institute of Anthropology, Anselm-Franz-von-Bentzel-Weg 7, 55128 Mainz, GermanyQuantitative Proteomics Group, Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, GermanyDepartment of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the NetherlandsBiology of Non-coding RNA Group, Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, GermanyGerm cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, piRNAs in mouse oocytes are relatively rare and dispensable. We present compelling evidence for strong Piwi and piRNA expression in oocytes of other mammals. Human fetal oocytes express PIWIL2 and transposon-enriched piRNAs. Oocytes in adult human ovary express PIWIL1 and PIWIL2, whereas those in bovine ovary only express PIWIL1. In human, macaque, and bovine ovaries, we find piRNAs that resemble testis-borne pachytene piRNAs. Isolated bovine follicular oocytes were shown to contain abundant, relatively short piRNAs that preferentially target transposable elements. Using label-free quantitative proteome analysis, we show that these maturing oocytes strongly and specifically express the PIWIL3 protein, alongside other, known piRNA-pathway components. A piRNA pool is still present in early bovine embryos, revealing a potential impact of piRNAs on mammalian embryogenesis. Our results reveal that there are highly dynamic piRNA pathways in mammalian oocytes and early embryos.http://www.sciencedirect.com/science/article/pii/S2211124715002399 |
| spellingShingle | Elke F. Roovers David Rosenkranz Mahdi Mahdipour Chung-Ting Han Nannan He Susana M. Chuva de Sousa Lopes Lucette A.J. van der Westerlaken Hans Zischler Falk Butter Bernard A.J. Roelen René F. Ketting Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos Cell Reports |
| title | Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos |
| title_full | Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos |
| title_fullStr | Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos |
| title_full_unstemmed | Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos |
| title_short | Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos |
| title_sort | piwi proteins and pirnas in mammalian oocytes and early embryos |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715002399 |
| work_keys_str_mv | AT elkefroovers piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT davidrosenkranz piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT mahdimahdipour piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT chungtinghan piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT nannanhe piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT susanamchuvadesousalopes piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT lucetteajvanderwesterlaken piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT hanszischler piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT falkbutter piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT bernardajroelen piwiproteinsandpirnasinmammalianoocytesandearlyembryos AT renefketting piwiproteinsandpirnasinmammalianoocytesandearlyembryos |