Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS
Understanding FA metabolism and lipid synthesis requires a lot of information about which FAs and lipids are formed within the cells. We focused on the use of deuterated substrates of 100 μM α-linolenic acid and linoleic acid to determine the relative amounts of their converted PUFAs and specific ph...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752030910X |
| _version_ | 1818647871008800768 |
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| author | Mai H. Philipsen Sanna Sämfors Per Malmberg Andrew G. Ewing |
| author_facet | Mai H. Philipsen Sanna Sämfors Per Malmberg Andrew G. Ewing |
| author_sort | Mai H. Philipsen |
| collection | DOAJ |
| description | Understanding FA metabolism and lipid synthesis requires a lot of information about which FAs and lipids are formed within the cells. We focused on the use of deuterated substrates of 100 μM α-linolenic acid and linoleic acid to determine the relative amounts of their converted PUFAs and specific phospholipids that are incorporated into cell plasma membranes. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was used to image and analyze lipids in model cell membranes with and without FA treatment. Because of its high spatial resolution, TOF-SIMS can be used to simultaneously provide both chemical information and distribution of various molecules in the sample surface down to the subcellular scale. Data obtained from this analysis of isotopes in the cell samples were used to calculate the relative amounts of long-chain PUFAs and phospholipids from their precursors, α-linolenic acid and linoleic acid. Our results show that the FA treatments induced an increase in the amounts of α-linolenic acid and linoleic acid and their long-chain conversion products. Moreover, an enhanced level of phospholipid turnover of these FAs in lipids such as phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols was also observed in the cell plasma membrane. |
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| format | Article |
| id | doaj.art-e7005ab644fd49d7aa6476508231b2b8 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T01:09:25Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e7005ab644fd49d7aa6476508231b2b82022-12-21T22:09:11ZengElsevierJournal of Lipid Research0022-22752018-11-01591120982107Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMSMai H. Philipsen0Sanna Sämfors1Per Malmberg2Andrew G. Ewing3Department of Chemistry and Chemical Engineering, University of Gothenburg, Gothenburg 412 96, Sweden; Go:IMS, Chalmers University of Technology University of Gothenburg, Gothenburg 412 96, SwedenDepartment of Chemistry and Chemical Engineering, University of Gothenburg, Gothenburg 412 96, Sweden; Go:IMS, Chalmers University of Technology University of Gothenburg, Gothenburg 412 96, SwedenDepartment of Chemistry and Chemical Engineering, University of Gothenburg, Gothenburg 412 96, Sweden; Go:IMS, Chalmers University of Technology University of Gothenburg, Gothenburg 412 96, SwedenTo whom correspondence should be addressed.; Department of Chemistry and Chemical Engineering, University of Gothenburg, Gothenburg 412 96, Sweden; Go:IMS, Chalmers University of Technology University of Gothenburg, Gothenburg 412 96, Sweden; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 412 96, SwedenUnderstanding FA metabolism and lipid synthesis requires a lot of information about which FAs and lipids are formed within the cells. We focused on the use of deuterated substrates of 100 μM α-linolenic acid and linoleic acid to determine the relative amounts of their converted PUFAs and specific phospholipids that are incorporated into cell plasma membranes. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) was used to image and analyze lipids in model cell membranes with and without FA treatment. Because of its high spatial resolution, TOF-SIMS can be used to simultaneously provide both chemical information and distribution of various molecules in the sample surface down to the subcellular scale. Data obtained from this analysis of isotopes in the cell samples were used to calculate the relative amounts of long-chain PUFAs and phospholipids from their precursors, α-linolenic acid and linoleic acid. Our results show that the FA treatments induced an increase in the amounts of α-linolenic acid and linoleic acid and their long-chain conversion products. Moreover, an enhanced level of phospholipid turnover of these FAs in lipids such as phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols was also observed in the cell plasma membrane.http://www.sciencedirect.com/science/article/pii/S002222752030910Xtime-of-flight secondary ion mass spectrometryphospholipidsα-linolenic acidlinoleic acidlipid turnoverfatty acids |
| spellingShingle | Mai H. Philipsen Sanna Sämfors Per Malmberg Andrew G. Ewing Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS Journal of Lipid Research time-of-flight secondary ion mass spectrometry phospholipids α-linolenic acid linoleic acid lipid turnover fatty acids |
| title | Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS |
| title_full | Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS |
| title_fullStr | Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS |
| title_full_unstemmed | Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS |
| title_short | Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS |
| title_sort | relative quantification of deuterated omega 3 and 6 fatty acids and their lipid turnover in pc12 cell membranes using tof sims |
| topic | time-of-flight secondary ion mass spectrometry phospholipids α-linolenic acid linoleic acid lipid turnover fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S002222752030910X |
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