The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats
Background and Aim: The aim of study was to investigate the effect of 8 weeks of moderate-intensity continuous training (MICT) and high intensity interval training (HIIT) on the GLUT4 gene expression in skeletal muscle of obese male rats. Materials and Methods: Twenty-eight male Wistar rats with 15...
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| Format: | Article |
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Arak Medical University
2018-08-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
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| Online Access: | http://jams.arakmu.ac.ir/article-1-5540-en.pdf |
| _version_ | 1818211415978147840 |
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| author | Mahnaz Najafi Mohammad Reza Asad Mostafa Rahimi Rahman Souri Ali Ahvar |
| author_facet | Mahnaz Najafi Mohammad Reza Asad Mostafa Rahimi Rahman Souri Ali Ahvar |
| author_sort | Mahnaz Najafi |
| collection | DOAJ |
| description | Background and Aim: The aim of study was to investigate the effect of 8 weeks of moderate-intensity continuous training (MICT) and high intensity interval training (HIIT) on the GLUT4 gene expression in skeletal muscle of obese male rats.
Materials and Methods: Twenty-eight male Wistar rats with 150 25 grams body weight were divided into base control (n = 6), obese control (n = 6), MICT (n = 8), and (n = 8) HIIT groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. After one week of familiarization, rats in training groups run on treadmill for 8 weeks and 5 days per week. Forty-eight hours after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. GLUT4 gene expression was measured by Real-Time PCR methods.
Findings: Results indicated that high fat diet for 8 weeks significantly increased rats body weight (p= 0.001), whereas both of MICT and HIIT training significantly decreased body weight (p= 0.001). Moreover, the MICT and HIIT had significant increase in GLUT4 gene expression in gastrocnemius muscle of obese rats (p = 0.001). Although, there were no differences between two experimental groups (p = 0.99).
Conclusion: According to the results, the MICT and HIIT increase gene expression of GLUT4 in mixed muscle fibers in obese rats. |
| first_indexed | 2024-12-12T05:32:09Z |
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| id | doaj.art-e7033c61040d4834aab14b2feca5f7e2 |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-12T05:32:09Z |
| publishDate | 2018-08-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-e7033c61040d4834aab14b2feca5f7e22022-12-22T00:36:17ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2018-08-0121498108The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male RatsMahnaz Najafi0Mohammad Reza Asad1Mostafa Rahimi2Rahman Souri3Ali Ahvar4Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Exercise Physiology, University of Payam Noor, Tehran, Iran.Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran. Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.Department of Exercise Physiology, University of Payam Noor, Tehran, Iran.Background and Aim: The aim of study was to investigate the effect of 8 weeks of moderate-intensity continuous training (MICT) and high intensity interval training (HIIT) on the GLUT4 gene expression in skeletal muscle of obese male rats. Materials and Methods: Twenty-eight male Wistar rats with 150 25 grams body weight were divided into base control (n = 6), obese control (n = 6), MICT (n = 8), and (n = 8) HIIT groups as study sample. At the beginning of the study, base control group rats were sacrificed. In other groups, rats underwent high fat regime diet for 8 weeks. After one week of familiarization, rats in training groups run on treadmill for 8 weeks and 5 days per week. Forty-eight hours after the last session, the rats were scarified and gastrocnemius muscle tissue sample were removed. GLUT4 gene expression was measured by Real-Time PCR methods. Findings: Results indicated that high fat diet for 8 weeks significantly increased rats body weight (p= 0.001), whereas both of MICT and HIIT training significantly decreased body weight (p= 0.001). Moreover, the MICT and HIIT had significant increase in GLUT4 gene expression in gastrocnemius muscle of obese rats (p = 0.001). Although, there were no differences between two experimental groups (p = 0.99). Conclusion: According to the results, the MICT and HIIT increase gene expression of GLUT4 in mixed muscle fibers in obese rats.http://jams.arakmu.ac.ir/article-1-5540-en.pdfGlucose transporter 4Moderate-intensity continuous trainingHigh intensity interval trainingSkeletal muscle |
| spellingShingle | Mahnaz Najafi Mohammad Reza Asad Mostafa Rahimi Rahman Souri Ali Ahvar The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Glucose transporter 4 Moderate-intensity continuous training High intensity interval training Skeletal muscle |
| title | The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats |
| title_full | The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats |
| title_fullStr | The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats |
| title_full_unstemmed | The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats |
| title_short | The Effect of Exercise with Different Intensity on Glucose Transporter 4 (GLUT4) Gene Expression in Skeletal Muscle of Obese Male Rats |
| title_sort | effect of exercise with different intensity on glucose transporter 4 glut4 gene expression in skeletal muscle of obese male rats |
| topic | Glucose transporter 4 Moderate-intensity continuous training High intensity interval training Skeletal muscle |
| url | http://jams.arakmu.ac.ir/article-1-5540-en.pdf |
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