Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions
The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we perfor...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/54740 |
| _version_ | 1811252970567761920 |
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| author | Michael J Prigge Matthieu Platre Nikita Kadakia Yi Zhang Kathleen Greenham Whitnie Szutu Bipin Kumar Pandey Rahul Arvind Bhosale Malcolm J Bennett Wolfgang Busch Mark Estelle |
| author_facet | Michael J Prigge Matthieu Platre Nikita Kadakia Yi Zhang Kathleen Greenham Whitnie Szutu Bipin Kumar Pandey Rahul Arvind Bhosale Malcolm J Bennett Wolfgang Busch Mark Estelle |
| author_sort | Michael J Prigge |
| collection | DOAJ |
| description | The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family. |
| first_indexed | 2024-04-12T16:42:45Z |
| format | Article |
| id | doaj.art-e703f4b6318a436e912ec23df16c7c33 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:42:45Z |
| publishDate | 2020-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e703f4b6318a436e912ec23df16c7c332022-12-22T03:24:42ZengeLife Sciences Publications LtdeLife2050-084X2020-02-01910.7554/eLife.54740Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functionsMichael J Prigge0https://orcid.org/0000-0003-0671-2538Matthieu Platre1Nikita Kadakia2Yi Zhang3Kathleen Greenham4https://orcid.org/0000-0001-7681-5263Whitnie Szutu5https://orcid.org/0000-0002-2083-7241Bipin Kumar Pandey6https://orcid.org/0000-0002-9614-1347Rahul Arvind Bhosale7https://orcid.org/0000-0001-6515-4922Malcolm J Bennett8Wolfgang Busch9Mark Estelle10https://orcid.org/0000-0002-2613-8652Section of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesPlant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United StatesSection of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesSection of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesSection of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesSection of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesPlant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United KingdomPlant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United KingdomPlant and Crop Sciences, School of Biosciences, University of Nottingham, Nottingham, United KingdomPlant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United StatesSection of Cell and Developmental Biology, University of California San Diego, La Jolla, United StatesThe TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.https://elifesciences.org/articles/54740auxinplant hormoneplant developmentplant |
| spellingShingle | Michael J Prigge Matthieu Platre Nikita Kadakia Yi Zhang Kathleen Greenham Whitnie Szutu Bipin Kumar Pandey Rahul Arvind Bhosale Malcolm J Bennett Wolfgang Busch Mark Estelle Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions eLife auxin plant hormone plant development plant |
| title | Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions |
| title_full | Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions |
| title_fullStr | Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions |
| title_full_unstemmed | Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions |
| title_short | Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions |
| title_sort | genetic analysis of the arabidopsis tir1 afb auxin receptors reveals both overlapping and specialized functions |
| topic | auxin plant hormone plant development plant |
| url | https://elifesciences.org/articles/54740 |
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