Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures
Nanotechnology plays an important role in many fields of science and the economy. A special example of nanostructures is silver nanoparticles (AgNPs) created following the principles of green chemistry, i.e., without the use of toxic reducing compounds. The common tomato (<i>Solanum lycopersic...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/5/2722 |
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| author | Miłosz Rutkowski Lidia Krzemińska-Fiedorowicz Gohar Khachatryan Karol Bulski Anna Kołton Karen Khachatryan |
| author_facet | Miłosz Rutkowski Lidia Krzemińska-Fiedorowicz Gohar Khachatryan Karol Bulski Anna Kołton Karen Khachatryan |
| author_sort | Miłosz Rutkowski |
| collection | DOAJ |
| description | Nanotechnology plays an important role in many fields of science and the economy. A special example of nanostructures is silver nanoparticles (AgNPs) created following the principles of green chemistry, i.e., without the use of toxic reducing compounds. The common tomato (<i>Solanum lycopersicum</i>) is a popular vegetable whose germination and growth process are studied by using, e.g., in vitro cultures. The aim of the experiment was to evaluate the inhibitory effect of the biodegradable gels containing silver nanoparticles on the development of microbial infection and to evaluate their influence on the germination degree of Tomato (<i>Solanum lycopersicum</i>) seeds in in vitro plant cultures. Based on macroscopic and microscopic observations, all experimental samples showed the presence of Gram-positive bacilli as well as mould fungi of the genus <i>Rhizopus</i>, <i>Alternaria</i> and <i>Aspergillus</i>. The study showed that the biocomponents containing silver nanoparticles obtained by using xylose as a reducing agent limit the development of microbial infection and stimulate the germination rate of tomato seeds. They could find their application as biodegradable raw materials in the production of modern disinfecting preparations for research in in vitro cultures. This study allowed to identify new research directions, especially to evaluate the metabolic regulation of seedlings treated with biodegradable silver nanoparticles. |
| first_indexed | 2024-03-09T20:47:10Z |
| format | Article |
| id | doaj.art-e7040892792f45318ce7e9d93303d4c6 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:47:10Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-e7040892792f45318ce7e9d93303d4c62023-11-23T22:45:17ZengMDPI AGApplied Sciences2076-34172022-03-01125272210.3390/app12052722Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro CulturesMiłosz Rutkowski0Lidia Krzemińska-Fiedorowicz1Gohar Khachatryan2Karol Bulski3Anna Kołton4Karen Khachatryan5Students’ Scientific Society of Biotechnologists “Helisa”, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandDepartment of Chemistry, Faculty of Food Technology, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandDepartment of Food Analysis and Evaluation of Food Quality, Faculty of Food Technology, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandFaculty of Agriculture and Economics, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandFaculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandDepartment of Chemistry, Faculty of Food Technology, University of Agriculture in Krakow, Mickiewicz Ave. 21, 31-120 Krakow, PolandNanotechnology plays an important role in many fields of science and the economy. A special example of nanostructures is silver nanoparticles (AgNPs) created following the principles of green chemistry, i.e., without the use of toxic reducing compounds. The common tomato (<i>Solanum lycopersicum</i>) is a popular vegetable whose germination and growth process are studied by using, e.g., in vitro cultures. The aim of the experiment was to evaluate the inhibitory effect of the biodegradable gels containing silver nanoparticles on the development of microbial infection and to evaluate their influence on the germination degree of Tomato (<i>Solanum lycopersicum</i>) seeds in in vitro plant cultures. Based on macroscopic and microscopic observations, all experimental samples showed the presence of Gram-positive bacilli as well as mould fungi of the genus <i>Rhizopus</i>, <i>Alternaria</i> and <i>Aspergillus</i>. The study showed that the biocomponents containing silver nanoparticles obtained by using xylose as a reducing agent limit the development of microbial infection and stimulate the germination rate of tomato seeds. They could find their application as biodegradable raw materials in the production of modern disinfecting preparations for research in in vitro cultures. This study allowed to identify new research directions, especially to evaluate the metabolic regulation of seedlings treated with biodegradable silver nanoparticles.https://www.mdpi.com/2076-3417/12/5/2722AgNPssodium alginatebacteriafungitomatoin vitro culture |
| spellingShingle | Miłosz Rutkowski Lidia Krzemińska-Fiedorowicz Gohar Khachatryan Karol Bulski Anna Kołton Karen Khachatryan Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures Applied Sciences AgNPs sodium alginate bacteria fungi tomato in vitro culture |
| title | Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures |
| title_full | Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures |
| title_fullStr | Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures |
| title_full_unstemmed | Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures |
| title_short | Biodegradable Silver Nanoparticles Gel and Its Impact on Tomato Seed Germination Rate in In Vitro Cultures |
| title_sort | biodegradable silver nanoparticles gel and its impact on tomato seed germination rate in in vitro cultures |
| topic | AgNPs sodium alginate bacteria fungi tomato in vitro culture |
| url | https://www.mdpi.com/2076-3417/12/5/2722 |
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