An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane
Isoprenoids are natural compounds essential for a great number of cellular functions. One of them is farnesol (FOH), which can reduce cell proliferation, but its low solubility in aqueous solvents limits its possible clinical use as a pharmacological tool. One alternative is the use of cyclodextrins...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/9/2735 |
| _version_ | 1797503774538858496 |
|---|---|
| author | Inmaculada de Dios-Pérez Álvaro González-Garcinuño Eva María Martín del Valle |
| author_facet | Inmaculada de Dios-Pérez Álvaro González-Garcinuño Eva María Martín del Valle |
| author_sort | Inmaculada de Dios-Pérez |
| collection | DOAJ |
| description | Isoprenoids are natural compounds essential for a great number of cellular functions. One of them is farnesol (FOH), which can reduce cell proliferation, but its low solubility in aqueous solvents limits its possible clinical use as a pharmacological tool. One alternative is the use of cyclodextrins (CDs) which house hydrophobic molecules forming inclusion complexes. To assess FOH potential application in anticancer treatments, Sulfobutylated β-cyclodextrin Sodium Salt (SBE-β-CD) was selected, due to it has high solubility, approbation by the FDA, and numerous studies that ensure its safety to be administered parenterally or orally without nephrotoxicity associated. The therapeutic action of farnesol and complex were studied in different carcinoma cells, compared with a normal cell line. Farnesol showed selectivity, affecting the viability of colon and liver cancer cells more than in breast cancer cells and fibroblasts. All cells suffered apoptosis after being treated with 150 μM of free FOH, but the complex reduced their cell viability between 50 and 75%. Similar results were obtained for both types of isomers, and the addition of phosphatidylcholine reverses this effect. Finally, cell cycle analysis corroborates the action of FOH as inducer of a G0/G1 phase; when the cells were treated using the complex form, this viability was reduced, reaching 50% in the case of colon and liver, 60% in fibroblasts, and only 75% in breast cancer. |
| first_indexed | 2024-03-10T03:55:09Z |
| format | Article |
| id | doaj.art-e7057e3e9e34436a8498cb0a5f3bb8b0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:55:09Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e7057e3e9e34436a8498cb0a5f3bb8b02023-11-23T08:48:51ZengMDPI AGMolecules1420-30492022-04-01279273510.3390/molecules27092735An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell MembraneInmaculada de Dios-Pérez0Álvaro González-Garcinuño1Eva María Martín del Valle2Department of Chemical Engineering, University of Salamanca, 37008 Salamanca, SpainDepartment of Chemical Engineering, University of Salamanca, 37008 Salamanca, SpainDepartment of Chemical Engineering, University of Salamanca, 37008 Salamanca, SpainIsoprenoids are natural compounds essential for a great number of cellular functions. One of them is farnesol (FOH), which can reduce cell proliferation, but its low solubility in aqueous solvents limits its possible clinical use as a pharmacological tool. One alternative is the use of cyclodextrins (CDs) which house hydrophobic molecules forming inclusion complexes. To assess FOH potential application in anticancer treatments, Sulfobutylated β-cyclodextrin Sodium Salt (SBE-β-CD) was selected, due to it has high solubility, approbation by the FDA, and numerous studies that ensure its safety to be administered parenterally or orally without nephrotoxicity associated. The therapeutic action of farnesol and complex were studied in different carcinoma cells, compared with a normal cell line. Farnesol showed selectivity, affecting the viability of colon and liver cancer cells more than in breast cancer cells and fibroblasts. All cells suffered apoptosis after being treated with 150 μM of free FOH, but the complex reduced their cell viability between 50 and 75%. Similar results were obtained for both types of isomers, and the addition of phosphatidylcholine reverses this effect. Finally, cell cycle analysis corroborates the action of FOH as inducer of a G0/G1 phase; when the cells were treated using the complex form, this viability was reduced, reaching 50% in the case of colon and liver, 60% in fibroblasts, and only 75% in breast cancer.https://www.mdpi.com/1420-3049/27/9/2735farnesolphosphatidylcholinephosphocholine cytidylyltransferasecyclodextrinssulfobutylated β-cyclodextrin |
| spellingShingle | Inmaculada de Dios-Pérez Álvaro González-Garcinuño Eva María Martín del Valle An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane Molecules farnesol phosphatidylcholine phosphocholine cytidylyltransferase cyclodextrins sulfobutylated β-cyclodextrin |
| title | An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane |
| title_full | An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane |
| title_fullStr | An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane |
| title_full_unstemmed | An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane |
| title_short | An Approach to Minimize Tumour Proliferation by Reducing the Formation of Components for Cell Membrane |
| title_sort | approach to minimize tumour proliferation by reducing the formation of components for cell membrane |
| topic | farnesol phosphatidylcholine phosphocholine cytidylyltransferase cyclodextrins sulfobutylated β-cyclodextrin |
| url | https://www.mdpi.com/1420-3049/27/9/2735 |
| work_keys_str_mv | AT inmaculadadediosperez anapproachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane AT alvarogonzalezgarcinuno anapproachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane AT evamariamartindelvalle anapproachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane AT inmaculadadediosperez approachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane AT alvarogonzalezgarcinuno approachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane AT evamariamartindelvalle approachtominimizetumourproliferationbyreducingtheformationofcomponentsforcellmembrane |