Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice
Objective To investigate the effect and potential mechanism of alpinetin (ALPN) on bleomycin (BLM)-induced pulmonary fibrosis in mice. Methods The mice were randomly divided into control group, model group (intratracheally instilled BLM), low-dose (L-ALPN group) and high-dose ALPN groups (H-ALPN gro...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-12-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-12-1827.pdf |
| _version_ | 1827391338306338816 |
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| author | SI Changxing, DING Yanyan, YIN Fengxian |
| author_facet | SI Changxing, DING Yanyan, YIN Fengxian |
| author_sort | SI Changxing, DING Yanyan, YIN Fengxian |
| collection | DOAJ |
| description | Objective To investigate the effect and potential mechanism of alpinetin (ALPN) on bleomycin (BLM)-induced pulmonary fibrosis in mice. Methods The mice were randomly divided into control group, model group (intratracheally instilled BLM), low-dose (L-ALPN group) and high-dose ALPN groups (H-ALPN group) (10 mg/kg or 30 mg/kg ALPN daily, respectively) with 10 in each. Lung tissues were collected, and the alveolar structure and pathological morphology weremicroscopied by HE and Masson staining; mRNA and protein expressions of collagen Ⅰ, TGF-β1, E-cadherin, α-SMA, p-PERK, PERK, CHOP and GRP78 in lung tissue were detected by RT-qPCR, immunohistochemistry and Western blot, respectively. Results Compared with control group, the lung of the model group showed fibrotic changes, and the expression of collagenⅠ, TGF-β1, α-SMA, p-PERK/PERK, CHOP and GRP78 in lung tissue was significantly increased (P＜0.01). E-cadherin expression was significantly decreased (P＜0.01). Compared with model group, pulmonary fibrosis was significantly alleviated in low and high doses ALPN groups, the expression of collagen Ⅰ, TGF-β1, α-SMA, p-PERK/PERK, CHOP and GRP78 in lung tissue were significantly decreased (P＜0.05 or P＜0.01), and the expressionof E-cadherin was significantly increased (P＜0.05 or P＜0.01). Conclusions ALPN may alleviate BLM-induced pulmonary fibrosis, and this effect may be attributed to the inhibition of endoplasmic reticulum stress. |
| first_indexed | 2024-03-08T17:09:19Z |
| format | Article |
| id | doaj.art-e7093fedb7ce420ba669bc110d2ad72b |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:09:19Z |
| publishDate | 2023-12-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-e7093fedb7ce420ba669bc110d2ad72b2024-01-04T01:10:35ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-12-0143121827183310.16352/j.issn.1001-6325.2023.12.1827Alpinetin alleviates bleomycin-induced pulmonary fibrosis in miceSI Changxing, DING Yanyan, YIN Fengxian0Department of Respiratory and Critical Care Medicine, Beijing Daxing District People's Hospital, Beijing 102600, ChinaObjective To investigate the effect and potential mechanism of alpinetin (ALPN) on bleomycin (BLM)-induced pulmonary fibrosis in mice. Methods The mice were randomly divided into control group, model group (intratracheally instilled BLM), low-dose (L-ALPN group) and high-dose ALPN groups (H-ALPN group) (10 mg/kg or 30 mg/kg ALPN daily, respectively) with 10 in each. Lung tissues were collected, and the alveolar structure and pathological morphology weremicroscopied by HE and Masson staining; mRNA and protein expressions of collagen Ⅰ, TGF-β1, E-cadherin, α-SMA, p-PERK, PERK, CHOP and GRP78 in lung tissue were detected by RT-qPCR, immunohistochemistry and Western blot, respectively. Results Compared with control group, the lung of the model group showed fibrotic changes, and the expression of collagenⅠ, TGF-β1, α-SMA, p-PERK/PERK, CHOP and GRP78 in lung tissue was significantly increased (P＜0.01). E-cadherin expression was significantly decreased (P＜0.01). Compared with model group, pulmonary fibrosis was significantly alleviated in low and high doses ALPN groups, the expression of collagen Ⅰ, TGF-β1, α-SMA, p-PERK/PERK, CHOP and GRP78 in lung tissue were significantly decreased (P＜0.05 or P＜0.01), and the expressionof E-cadherin was significantly increased (P＜0.05 or P＜0.01). Conclusions ALPN may alleviate BLM-induced pulmonary fibrosis, and this effect may be attributed to the inhibition of endoplasmic reticulum stress.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-12-1827.pdfalpinetin|bleomycin|endoplasmic reticulum stress|pulmonary fibrosis |
| spellingShingle | SI Changxing, DING Yanyan, YIN Fengxian Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice Jichu yixue yu linchuang alpinetin|bleomycin|endoplasmic reticulum stress|pulmonary fibrosis |
| title | Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice |
| title_full | Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice |
| title_fullStr | Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice |
| title_full_unstemmed | Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice |
| title_short | Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice |
| title_sort | alpinetin alleviates bleomycin induced pulmonary fibrosis in mice |
| topic | alpinetin|bleomycin|endoplasmic reticulum stress|pulmonary fibrosis |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-12-1827.pdf |
| work_keys_str_mv | AT sichangxingdingyanyanyinfengxian alpinetinalleviatesbleomycininducedpulmonaryfibrosisinmice |