Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice
Introduction: Particulate air pollution, containing nanoparticles, enhances the risk of pediatric allergic diseases that is potentially associated with disruption of neonatal immune system. Previous studies have revealed that maternal exposure to carbon black nanoparticles (CB-NP) disturbs the devel...
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Frontiers Media S.A.
2021-08-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2021.700392/full |
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| author | Atsuto Onoda Atsuto Onoda Atsuto Onoda Saki Okamoto Ryuhei Shimizu Yasser S. El-Sayed Shiho Watanabe Shuhei Ogawa Shuhei Ogawa Ryo Abe Ryo Abe Masao Kamimura Kohei Soga Ken Tachibana Ken Tachibana Ken Tachibana Ken Takeda Ken Takeda Ken Takeda Masakazu Umezawa Masakazu Umezawa Masakazu Umezawa |
| author_facet | Atsuto Onoda Atsuto Onoda Atsuto Onoda Saki Okamoto Ryuhei Shimizu Yasser S. El-Sayed Shiho Watanabe Shuhei Ogawa Shuhei Ogawa Ryo Abe Ryo Abe Masao Kamimura Kohei Soga Ken Tachibana Ken Tachibana Ken Tachibana Ken Takeda Ken Takeda Ken Takeda Masakazu Umezawa Masakazu Umezawa Masakazu Umezawa |
| author_sort | Atsuto Onoda |
| collection | DOAJ |
| description | Introduction: Particulate air pollution, containing nanoparticles, enhances the risk of pediatric allergic diseases that is potentially associated with disruption of neonatal immune system. Previous studies have revealed that maternal exposure to carbon black nanoparticles (CB-NP) disturbs the development of the lymphoid tissues in newborns. Interestingly, the CB-NP-induced immune profiles were observed to be different depending on the gestational period of exposure. It is important to identify the critical exposure period to prevent toxic effects of nanoparticles on the development of the immune system. Therefore, the present study was aimed to investigate the effect of CB-NP on the development of neonatal lymphoid tissues in mice, depending on the gestational period of exposure.Methods: Pregnant ICR mice were treated with a suspension of CB-NP (95 μg/kg body weight) by intranasal instillation; the suspension was administered twice during each gestational period as follows: the pre-implantation period (gestational days 4 and 5), organogenesis period (gestational days 8 and 9), and fetal developmental period (gestational days 15 and 16). The spleen and thymus were collected from offspring mice at 1, 3, and 5-days post-partum. Splenocyte and thymocyte phenotypes were examined by flow cytometry. Gene expression in the spleen was examined by quantitative reverse transcription-polymerase chain reaction.Results: The numbers of total splenocytes and splenic CD3−B220− phenotype (non-T/non-B lymphocytes) in offspring on postnatal day 5 were significantly increased after exposure to CB-NP during the organogenesis period compared with other gestational periods of exposure and control (no exposure). In contrast, expression levels of mRNA associated with chemotaxis and differentiation of immune cells in the spleen were not affected by CB-NP exposure during any gestational period.Conclusion: The organogenesis period was the most susceptible period to CB-NP exposure with respect to lymphoid tissue development. Moreover, the findings of the present and previous studies suggested that long-term exposure to CB-NP across multiple gestational periods including the organogenesis period, rather than acute exposure only organogenesis period, may more severely affect the development of the immune system. |
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| spelling | doaj.art-e71042dd49434d79acb849ba691242b42022-12-21T21:31:10ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802021-08-01310.3389/ftox.2021.700392700392Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in MiceAtsuto Onoda0Atsuto Onoda1Atsuto Onoda2Saki Okamoto3Ryuhei Shimizu4Yasser S. El-Sayed5Shiho Watanabe6Shuhei Ogawa7Shuhei Ogawa8Ryo Abe9Ryo Abe10Masao Kamimura11Kohei Soga12Ken Tachibana13Ken Tachibana14Ken Tachibana15Ken Takeda16Ken Takeda17Ken Takeda18Masakazu Umezawa19Masakazu Umezawa20Masakazu Umezawa21The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Sanyoonoda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanFaculty of Veterinary Medicine, Damanhour University, Damanhour, EgyptResearch Institute for Biomedical Sciences, Tokyo University of Science, Noda, JapanThe Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science, Noda, JapanResearch Institute for Biomedical Sciences, Tokyo University of Science, Noda, JapanResearch Institute for Biomedical Sciences, Tokyo University of Science, Noda, JapanAdvanced Comprehensive Research Center, Teikyo University, Hachioji, JapanDepartment of Materials Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, Katsushika, JapanDepartment of Materials Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, Katsushika, JapanThe Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Sanyoonoda, JapanThe Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Sanyoonoda, JapanThe Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science, Noda, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, JapanDepartment of Materials Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, Katsushika, JapanIntroduction: Particulate air pollution, containing nanoparticles, enhances the risk of pediatric allergic diseases that is potentially associated with disruption of neonatal immune system. Previous studies have revealed that maternal exposure to carbon black nanoparticles (CB-NP) disturbs the development of the lymphoid tissues in newborns. Interestingly, the CB-NP-induced immune profiles were observed to be different depending on the gestational period of exposure. It is important to identify the critical exposure period to prevent toxic effects of nanoparticles on the development of the immune system. Therefore, the present study was aimed to investigate the effect of CB-NP on the development of neonatal lymphoid tissues in mice, depending on the gestational period of exposure.Methods: Pregnant ICR mice were treated with a suspension of CB-NP (95 μg/kg body weight) by intranasal instillation; the suspension was administered twice during each gestational period as follows: the pre-implantation period (gestational days 4 and 5), organogenesis period (gestational days 8 and 9), and fetal developmental period (gestational days 15 and 16). The spleen and thymus were collected from offspring mice at 1, 3, and 5-days post-partum. Splenocyte and thymocyte phenotypes were examined by flow cytometry. Gene expression in the spleen was examined by quantitative reverse transcription-polymerase chain reaction.Results: The numbers of total splenocytes and splenic CD3−B220− phenotype (non-T/non-B lymphocytes) in offspring on postnatal day 5 were significantly increased after exposure to CB-NP during the organogenesis period compared with other gestational periods of exposure and control (no exposure). In contrast, expression levels of mRNA associated with chemotaxis and differentiation of immune cells in the spleen were not affected by CB-NP exposure during any gestational period.Conclusion: The organogenesis period was the most susceptible period to CB-NP exposure with respect to lymphoid tissue development. Moreover, the findings of the present and previous studies suggested that long-term exposure to CB-NP across multiple gestational periods including the organogenesis period, rather than acute exposure only organogenesis period, may more severely affect the development of the immune system.https://www.frontiersin.org/articles/10.3389/ftox.2021.700392/fullcarbon black nanoparticlesair pollutionnanomaterialneonateslymphoid tissue developmentimmune response |
| spellingShingle | Atsuto Onoda Atsuto Onoda Atsuto Onoda Saki Okamoto Ryuhei Shimizu Yasser S. El-Sayed Shiho Watanabe Shuhei Ogawa Shuhei Ogawa Ryo Abe Ryo Abe Masao Kamimura Kohei Soga Ken Tachibana Ken Tachibana Ken Tachibana Ken Takeda Ken Takeda Ken Takeda Masakazu Umezawa Masakazu Umezawa Masakazu Umezawa Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice Frontiers in Toxicology carbon black nanoparticles air pollution nanomaterial neonates lymphoid tissue development immune response |
| title | Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice |
| title_full | Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice |
| title_fullStr | Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice |
| title_full_unstemmed | Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice |
| title_short | Effect of Carbon Black Nanoparticle on Neonatal Lymphoid Tissues Depending on the Gestational Period of Exposure in Mice |
| title_sort | effect of carbon black nanoparticle on neonatal lymphoid tissues depending on the gestational period of exposure in mice |
| topic | carbon black nanoparticles air pollution nanomaterial neonates lymphoid tissue development immune response |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2021.700392/full |
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