Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives
Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has been a major global threat to the public health. Since lung is the major target of SARS-CoV-2, the infection can lead to respiratory distress...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022021466 |
| _version_ | 1797991787495686144 |
|---|---|
| author | Kang-Sheng Liu Xiao-Dong Mao Wenjing Ni Tai-Ping Li |
| author_facet | Kang-Sheng Liu Xiao-Dong Mao Wenjing Ni Tai-Ping Li |
| author_sort | Kang-Sheng Liu |
| collection | DOAJ |
| description | Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has been a major global threat to the public health. Since lung is the major target of SARS-CoV-2, the infection can lead to respiratory distress syndrome (RDS), multiple organ failure (MOF), and even death. The studies on viral structure and infection mechanism have found that angiotensin-converting enzyme 2 (ACE2), a pivotal enzyme affecting the organ-targeting in the RAS system, is the receptor of the SARS-CoV-2 virus. Currently, the detection of SARSCoV-2 is mainly achieved using open plate real-time reverse-transcription polymerase chain reaction (RT-PCR). While open plate method has some limitations, such as a high false-negative rate, cumbersome manual operation, aerosol pollution and leakage risks. Therefore, a convenient method to rapidly detect SARS-CoV-2 virus is urgently and extremely required for timely epidemic control with the limited resources. In this review, the current real-time methods and principles for novel coronavirus detection are summarized, with the aim to provide a reference for real-time screening of coronavirus in areas with insufficient detection capacity and inadequate medical resources. The development and establishment of a rapid, simple, sensitive and specific system to detect SARS-CoV-2 is of vital importance for distinct diagnosis and effective treatment of the virus, especially in the flu season. |
| first_indexed | 2024-04-11T08:57:47Z |
| format | Article |
| id | doaj.art-e71332a0af774737ba1ae256541bbd18 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T08:57:47Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e71332a0af774737ba1ae256541bbd182022-12-22T04:33:09ZengElsevierHeliyon2405-84402022-10-01810e10858Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectivesKang-Sheng Liu0Xiao-Dong Mao1Wenjing Ni2Tai-Ping Li3Department of Clinical Laboratory, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210029, ChinaDepartment of Endocrinology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, China; Key Laboratory of TCM Syndrome & Treatment of Yingbing of State Administration of Traditional Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, ChinaDepartment of Endocrinology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, China; Key Laboratory of TCM Syndrome & Treatment of Yingbing of State Administration of Traditional Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, ChinaDepartment of Neuro-Psychiatric Institute, The Affiliated Nanjing Brain Hospital of Nanjing Medical University, Nanjing 210029, China; Corresponding author.Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has been a major global threat to the public health. Since lung is the major target of SARS-CoV-2, the infection can lead to respiratory distress syndrome (RDS), multiple organ failure (MOF), and even death. The studies on viral structure and infection mechanism have found that angiotensin-converting enzyme 2 (ACE2), a pivotal enzyme affecting the organ-targeting in the RAS system, is the receptor of the SARS-CoV-2 virus. Currently, the detection of SARSCoV-2 is mainly achieved using open plate real-time reverse-transcription polymerase chain reaction (RT-PCR). While open plate method has some limitations, such as a high false-negative rate, cumbersome manual operation, aerosol pollution and leakage risks. Therefore, a convenient method to rapidly detect SARS-CoV-2 virus is urgently and extremely required for timely epidemic control with the limited resources. In this review, the current real-time methods and principles for novel coronavirus detection are summarized, with the aim to provide a reference for real-time screening of coronavirus in areas with insufficient detection capacity and inadequate medical resources. The development and establishment of a rapid, simple, sensitive and specific system to detect SARS-CoV-2 is of vital importance for distinct diagnosis and effective treatment of the virus, especially in the flu season.http://www.sciencedirect.com/science/article/pii/S2405844022021466Novel coronavirusSARS-CoV-2Gene sequencingNucleic acid testingGene editing techniques |
| spellingShingle | Kang-Sheng Liu Xiao-Dong Mao Wenjing Ni Tai-Ping Li Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives Heliyon Novel coronavirus SARS-CoV-2 Gene sequencing Nucleic acid testing Gene editing techniques |
| title | Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives |
| title_full | Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives |
| title_fullStr | Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives |
| title_full_unstemmed | Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives |
| title_short | Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives |
| title_sort | laboratory detection of sars cov 2 a review of the current literature and future perspectives |
| topic | Novel coronavirus SARS-CoV-2 Gene sequencing Nucleic acid testing Gene editing techniques |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022021466 |
| work_keys_str_mv | AT kangshengliu laboratorydetectionofsarscov2areviewofthecurrentliteratureandfutureperspectives AT xiaodongmao laboratorydetectionofsarscov2areviewofthecurrentliteratureandfutureperspectives AT wenjingni laboratorydetectionofsarscov2areviewofthecurrentliteratureandfutureperspectives AT taipingli laboratorydetectionofsarscov2areviewofthecurrentliteratureandfutureperspectives |