| Summary: | In this study, we proposed a novel Ru(bpy)<sub>3</sub><sup>2+</sup>-Au-WS<sub>2</sub> nanocomposite (Ru-Au-WS<sub>2</sub> NCs) nano-hybrid electrochemiluminescence (ECL) probe for the highly sensitive detection of carcinoembryonic antigen (CEA). This system utilizes Au nanoparticles (Au NPs) as a bridge to graft the high-performance of a Ru(bpy)<sub>3</sub><sup>2+</sup> ECL emitter and WS<sub>2</sub> nanosheet with excellent electrochemical performance into an ECL platform, which shows outstanding anodic ECL performance and biosensing platform due to the synergetic effect and biocompatibility of Au NPs and WS<sub>2</sub> nanosheet. Because the ECL intensity of Ru(bpy)<sub>3</sub><sup>2+</sup> is sensitively affected by the antibody-antigen insulator, a preferable linear dependence was obtained in the concentration range of CEA from 1 pg·mL<sup>−1</sup> to 350 ng·mL<sup>−1</sup> with high selectivity (LOD of 0.3 pg·mL<sup>−1</sup>, S/N = 3). Moreover, the ECL platform had good reproducibility and stability and exhibited excellent anti-interference performance in the detection process of CEA. We believe that the platform we have developed can expand the opportunities for the detection of additional high specificity-related antibodies/antigens and demonstrate broad prospects for disease diagnosis and biochemical research.
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