Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials
In this study, we proposed a novel Ru(bpy)<sub>3</sub><sup>2+</sup>-Au-WS<sub>2</sub> nanocomposite (Ru-Au-WS<sub>2</sub> NCs) nano-hybrid electrochemiluminescence (ECL) probe for the highly sensitive detection of carcinoembryonic antigen (CEA). This s...
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/1/58 |
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| author | Qile Li Ke Xu Haipeng Zhang Zengguang Huang Chao Xu Zhen Zhou Huaping Peng Linxing Shi |
| author_facet | Qile Li Ke Xu Haipeng Zhang Zengguang Huang Chao Xu Zhen Zhou Huaping Peng Linxing Shi |
| author_sort | Qile Li |
| collection | DOAJ |
| description | In this study, we proposed a novel Ru(bpy)<sub>3</sub><sup>2+</sup>-Au-WS<sub>2</sub> nanocomposite (Ru-Au-WS<sub>2</sub> NCs) nano-hybrid electrochemiluminescence (ECL) probe for the highly sensitive detection of carcinoembryonic antigen (CEA). This system utilizes Au nanoparticles (Au NPs) as a bridge to graft the high-performance of a Ru(bpy)<sub>3</sub><sup>2+</sup> ECL emitter and WS<sub>2</sub> nanosheet with excellent electrochemical performance into an ECL platform, which shows outstanding anodic ECL performance and biosensing platform due to the synergetic effect and biocompatibility of Au NPs and WS<sub>2</sub> nanosheet. Because the ECL intensity of Ru(bpy)<sub>3</sub><sup>2+</sup> is sensitively affected by the antibody-antigen insulator, a preferable linear dependence was obtained in the concentration range of CEA from 1 pg·mL<sup>−1</sup> to 350 ng·mL<sup>−1</sup> with high selectivity (LOD of 0.3 pg·mL<sup>−1</sup>, S/N = 3). Moreover, the ECL platform had good reproducibility and stability and exhibited excellent anti-interference performance in the detection process of CEA. We believe that the platform we have developed can expand the opportunities for the detection of additional high specificity-related antibodies/antigens and demonstrate broad prospects for disease diagnosis and biochemical research. |
| first_indexed | 2024-03-09T13:25:47Z |
| format | Article |
| id | doaj.art-e717abce211a46989c5400e44555dfa8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:25:47Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e717abce211a46989c5400e44555dfa82023-11-30T21:24:53ZengMDPI AGBiosensors2079-63742022-12-011315810.3390/bios13010058Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid MaterialsQile Li0Ke Xu1Haipeng Zhang2Zengguang Huang3Chao Xu4Zhen Zhou5Huaping Peng6Linxing Shi7School of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaFujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou 350004, ChinaSchool of Science, Jiangsu Ocean University, Lianyungang 222005, ChinaIn this study, we proposed a novel Ru(bpy)<sub>3</sub><sup>2+</sup>-Au-WS<sub>2</sub> nanocomposite (Ru-Au-WS<sub>2</sub> NCs) nano-hybrid electrochemiluminescence (ECL) probe for the highly sensitive detection of carcinoembryonic antigen (CEA). This system utilizes Au nanoparticles (Au NPs) as a bridge to graft the high-performance of a Ru(bpy)<sub>3</sub><sup>2+</sup> ECL emitter and WS<sub>2</sub> nanosheet with excellent electrochemical performance into an ECL platform, which shows outstanding anodic ECL performance and biosensing platform due to the synergetic effect and biocompatibility of Au NPs and WS<sub>2</sub> nanosheet. Because the ECL intensity of Ru(bpy)<sub>3</sub><sup>2+</sup> is sensitively affected by the antibody-antigen insulator, a preferable linear dependence was obtained in the concentration range of CEA from 1 pg·mL<sup>−1</sup> to 350 ng·mL<sup>−1</sup> with high selectivity (LOD of 0.3 pg·mL<sup>−1</sup>, S/N = 3). Moreover, the ECL platform had good reproducibility and stability and exhibited excellent anti-interference performance in the detection process of CEA. We believe that the platform we have developed can expand the opportunities for the detection of additional high specificity-related antibodies/antigens and demonstrate broad prospects for disease diagnosis and biochemical research.https://www.mdpi.com/2079-6374/13/1/58Ru(bpy)<sub>3</sub><sup>2+</sup>WS<sub>2</sub>Au NPselectrochemiluminescencecarcinoembryonic antigen |
| spellingShingle | Qile Li Ke Xu Haipeng Zhang Zengguang Huang Chao Xu Zhen Zhou Huaping Peng Linxing Shi Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials Biosensors Ru(bpy)<sub>3</sub><sup>2+</sup> WS<sub>2</sub> Au NPs electrochemiluminescence carcinoembryonic antigen |
| title | Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials |
| title_full | Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials |
| title_fullStr | Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials |
| title_full_unstemmed | Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials |
| title_short | Ultrasensitive Electrochemiluminescence Immunoassay Based on Signal Amplification of 0D Au—2D WS<sub>2</sub> Nano-Hybrid Materials |
| title_sort | ultrasensitive electrochemiluminescence immunoassay based on signal amplification of 0d au 2d ws sub 2 sub nano hybrid materials |
| topic | Ru(bpy)<sub>3</sub><sup>2+</sup> WS<sub>2</sub> Au NPs electrochemiluminescence carcinoembryonic antigen |
| url | https://www.mdpi.com/2079-6374/13/1/58 |
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