Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat
Objective: To explore the effects of different concentrations of Rosa roxburghii Tratt powder on antioxidant stress injury of skeletal muscle in overtrained rats. Methods: The rats were divided into the control group (C group), exercise group (HT group), low concentration Rosa roxburghii Tratt powde...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090352 |
| _version_ | 1797983586172796928 |
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| author | Shuaijun ZHANG Yuemei TANG Dading ZHANG Jin ZHANG |
| author_facet | Shuaijun ZHANG Yuemei TANG Dading ZHANG Jin ZHANG |
| author_sort | Shuaijun ZHANG |
| collection | DOAJ |
| description | Objective: To explore the effects of different concentrations of Rosa roxburghii Tratt powder on antioxidant stress injury of skeletal muscle in overtrained rats. Methods: The rats were divided into the control group (C group), exercise group (HT group), low concentration Rosa roxburghii Tratt powder exercise group (L-HT group) and high concentration Rosa roxburghii Tratt powder exercise group (H-HT group). HT, L-HT and H-HT groups underwent treadmill training with increasing load for 6 weeks, respectively. During the training, L-HT group and H-HT group were intragastrically administered with 20 and 50 mg/mL Rosa roxburghii Tratt powder solution at the dose of 20 mL·kg−1·d−1, respectively. Group C and HT were given the same dose of saline. The samples collected 24 hours after last training were used to measure serum creatine kinase (CK), interleukin -1β (IL-1β) and tumor necrosis factor-α (TNF-α). The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) and glutathione (GSH) in skeletal muscle were determined. The expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1(HO-1), B cell lymphoma factor-2 (Bcl-2) and Bcl-2-related X protein(Bax) in skeletal muscle was detected. Results: Compared with the HT group, the activity of CK decreased (P<0.05), the activity of SOD increased (P<0.05), the contents of IL-1β, TNF-α and MDA decreased (P<0.05), the concentration of GSH increased (P<0.05), the expressions of Nrf2, HO-1 and Bcl-2 proteins increased (P<0.05), and the expression of Bax protein decreased in L-HT group (P<0.05). The activity of CK decreased (P<0.01), the activity of SOD increased (P<0.01), the contents of IL-1β, TNF-α and MDA decreased (P<0.01), the concentration of GSH increased (P<0.01), the expressions of Nrf2, HO-1 and Bcl-2 proteins increased (P<0.01), and the expression of Bax protein decreased in H-HT group (P<0.01). Compared with the L-HT group, the activity of CK decreased (P<0.05), the activity of SOD increased (P<0.05), the contents of IL-1β, TNF-α and MDA decreased (P<0.05), the expression of Nrf-2, HO-1 and Bcl-2 protein increased (P<0.05), and the expression of Bax protein decreased in H-HT group (P<0.05). Conclusion: Rosa roxburghii Tratt powder had a good protective effect on oxidative stress injury induced by overtraining in rats, and there was a dose-response relationship between the dosage of Rosa roxburghii Tratt powder and antioxidative stress injury in a certain range. |
| first_indexed | 2024-04-11T06:49:39Z |
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| id | doaj.art-e7192b4852a24e39b68ee4b261e57187 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:49:39Z |
| publishDate | 2022-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-e7192b4852a24e39b68ee4b261e571872022-12-22T04:39:15ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-06-01431233834610.13386/j.issn1002-0306.20210903522021090352-12Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained RatShuaijun ZHANG0Yuemei TANG1Dading ZHANG2Jin ZHANG3Science and Technology College Gannan Normal University, Ganzhou 341000, ChinaScience and Technology College Gannan Normal University, Ganzhou 341000, ChinaScience and Technology College Gannan Normal University, Ganzhou 341000, ChinaThe First People’s Hospital of Pingdingshan, Pingdingshan 467000, ChinaObjective: To explore the effects of different concentrations of Rosa roxburghii Tratt powder on antioxidant stress injury of skeletal muscle in overtrained rats. Methods: The rats were divided into the control group (C group), exercise group (HT group), low concentration Rosa roxburghii Tratt powder exercise group (L-HT group) and high concentration Rosa roxburghii Tratt powder exercise group (H-HT group). HT, L-HT and H-HT groups underwent treadmill training with increasing load for 6 weeks, respectively. During the training, L-HT group and H-HT group were intragastrically administered with 20 and 50 mg/mL Rosa roxburghii Tratt powder solution at the dose of 20 mL·kg−1·d−1, respectively. Group C and HT were given the same dose of saline. The samples collected 24 hours after last training were used to measure serum creatine kinase (CK), interleukin -1β (IL-1β) and tumor necrosis factor-α (TNF-α). The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) and glutathione (GSH) in skeletal muscle were determined. The expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1(HO-1), B cell lymphoma factor-2 (Bcl-2) and Bcl-2-related X protein(Bax) in skeletal muscle was detected. Results: Compared with the HT group, the activity of CK decreased (P<0.05), the activity of SOD increased (P<0.05), the contents of IL-1β, TNF-α and MDA decreased (P<0.05), the concentration of GSH increased (P<0.05), the expressions of Nrf2, HO-1 and Bcl-2 proteins increased (P<0.05), and the expression of Bax protein decreased in L-HT group (P<0.05). The activity of CK decreased (P<0.01), the activity of SOD increased (P<0.01), the contents of IL-1β, TNF-α and MDA decreased (P<0.01), the concentration of GSH increased (P<0.01), the expressions of Nrf2, HO-1 and Bcl-2 proteins increased (P<0.01), and the expression of Bax protein decreased in H-HT group (P<0.01). Compared with the L-HT group, the activity of CK decreased (P<0.05), the activity of SOD increased (P<0.05), the contents of IL-1β, TNF-α and MDA decreased (P<0.05), the expression of Nrf-2, HO-1 and Bcl-2 protein increased (P<0.05), and the expression of Bax protein decreased in H-HT group (P<0.05). Conclusion: Rosa roxburghii Tratt powder had a good protective effect on oxidative stress injury induced by overtraining in rats, and there was a dose-response relationship between the dosage of Rosa roxburghii Tratt powder and antioxidative stress injury in a certain range.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090352rosa roxburghii tratt powderovertrainingskeletal muscleoxidative stressdamage |
| spellingShingle | Shuaijun ZHANG Yuemei TANG Dading ZHANG Jin ZHANG Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat Shipin gongye ke-ji rosa roxburghii tratt powder overtraining skeletal muscle oxidative stress damage |
| title | Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat |
| title_full | Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat |
| title_fullStr | Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat |
| title_full_unstemmed | Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat |
| title_short | Effect of Rosa roxburghii Tratt Powder on Alleviating Oxidative Stress Injury of Skeletal Muscle in Overtrained Rat |
| title_sort | effect of rosa roxburghii tratt powder on alleviating oxidative stress injury of skeletal muscle in overtrained rat |
| topic | rosa roxburghii tratt powder overtraining skeletal muscle oxidative stress damage |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090352 |
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