Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats
Abstract Background We investigated whether the long-term consumption of a symbiotic formulation with Lactobacillus fermentum (probiotic) and β-glucan from cauliflower mushroom (prebiotic) would delay the progression of post-menopausal symptoms in ovariectomized (OVX) rats and explored their mechani...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Genes & Nutrition |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12263-017-0585-z |
| _version_ | 1811240899681714176 |
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| author | Seong-Yeop Jeong Suna Kang Cao Shi Hua Zhang Ting Sunmin Park |
| author_facet | Seong-Yeop Jeong Suna Kang Cao Shi Hua Zhang Ting Sunmin Park |
| author_sort | Seong-Yeop Jeong |
| collection | DOAJ |
| description | Abstract Background We investigated whether the long-term consumption of a symbiotic formulation with Lactobacillus fermentum (probiotic) and β-glucan from cauliflower mushroom (prebiotic) would delay the progression of post-menopausal symptoms in ovariectomized (OVX) rats and explored their mechanisms of action, including changes in gut microbiota. Methods OVX rats were fed with high-fat diets containing 1% dextrin (control), 1% lyophilized cauliflower mushroom extract (CFM), 0.1% L. fermentum JS (LFE), 1% CFM plus 0.1% LFE (CFLF), or 30 μg 17β-estradiol/kg body weight (positive-control) for 8 weeks. Results CFM contained 95.8% β-glucans. OVX increased the ratio of Firmicutes and Bacteroidetes in the large intestines. Only CFLF lowered tail skin temperature without increasing serum 17β-estradiol and uterine index. Visceral fat mass was lower in CFLF and positive-control groups by increasing daily energy expenditure and fat oxidation. Dyslipidemia induced by OVX was improved by CFM and CFLF as much as in the positive-control group. Homeostasis model assessment estimate of insulin resistance was lower in CFLF than in the positive-control. Hepatic insulin signaling (pAkt➔GSK-3β) was potentiated in the ascending order of the control, LFE, CFM, CFLF, and positive-control. AMPK phosphorylation showed similar patterns of hepatic insulin signaling but LFE increased it more than CFM. The changes in gut microbiota were prevented by CFLF in OVX rats, and the ratio of Firmicutes and Bacteroidetes in the CFLF was similar to the positive-control group. Conclusion OVX changed gut microbiota and was associated with menopausal symptoms; however, the synbiotics, CFM and LFE, prevented menopausal symptoms and improved the gut microbiota in estrogen-deficient rats. |
| first_indexed | 2024-04-12T13:27:46Z |
| format | Article |
| id | doaj.art-e719642874dc439ebfed7404523e1536 |
| institution | Directory Open Access Journal |
| issn | 1555-8932 1865-3499 |
| language | English |
| last_indexed | 2024-04-12T13:27:46Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Genes & Nutrition |
| spelling | doaj.art-e719642874dc439ebfed7404523e15362022-12-22T03:31:16ZengBMCGenes & Nutrition1555-89321865-34992017-11-0112111210.1186/s12263-017-0585-zSynbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient ratsSeong-Yeop Jeong0Suna Kang1Cao Shi Hua2Zhang Ting3Sunmin Park4Department of R&D, Microbial Institute for Fermentation IndustryDepartment of Food & Nutrition, Obesity/Diabetes Center, Hoseo UniversityDepartment of Food & Nutrition, Obesity/Diabetes Center, Hoseo UniversityDepartment of Food & Nutrition, Obesity/Diabetes Center, Hoseo UniversityDepartment of Food & Nutrition, Obesity/Diabetes Center, Hoseo UniversityAbstract Background We investigated whether the long-term consumption of a symbiotic formulation with Lactobacillus fermentum (probiotic) and β-glucan from cauliflower mushroom (prebiotic) would delay the progression of post-menopausal symptoms in ovariectomized (OVX) rats and explored their mechanisms of action, including changes in gut microbiota. Methods OVX rats were fed with high-fat diets containing 1% dextrin (control), 1% lyophilized cauliflower mushroom extract (CFM), 0.1% L. fermentum JS (LFE), 1% CFM plus 0.1% LFE (CFLF), or 30 μg 17β-estradiol/kg body weight (positive-control) for 8 weeks. Results CFM contained 95.8% β-glucans. OVX increased the ratio of Firmicutes and Bacteroidetes in the large intestines. Only CFLF lowered tail skin temperature without increasing serum 17β-estradiol and uterine index. Visceral fat mass was lower in CFLF and positive-control groups by increasing daily energy expenditure and fat oxidation. Dyslipidemia induced by OVX was improved by CFM and CFLF as much as in the positive-control group. Homeostasis model assessment estimate of insulin resistance was lower in CFLF than in the positive-control. Hepatic insulin signaling (pAkt➔GSK-3β) was potentiated in the ascending order of the control, LFE, CFM, CFLF, and positive-control. AMPK phosphorylation showed similar patterns of hepatic insulin signaling but LFE increased it more than CFM. The changes in gut microbiota were prevented by CFLF in OVX rats, and the ratio of Firmicutes and Bacteroidetes in the CFLF was similar to the positive-control group. Conclusion OVX changed gut microbiota and was associated with menopausal symptoms; however, the synbiotics, CFM and LFE, prevented menopausal symptoms and improved the gut microbiota in estrogen-deficient rats.http://link.springer.com/article/10.1186/s12263-017-0585-zCauliflower mushroomβ-GlucanLactobacillus fermentumGut microbiotaOvariectomySynbiotics |
| spellingShingle | Seong-Yeop Jeong Suna Kang Cao Shi Hua Zhang Ting Sunmin Park Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats Genes & Nutrition Cauliflower mushroom β-Glucan Lactobacillus fermentum Gut microbiota Ovariectomy Synbiotics |
| title | Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats |
| title_full | Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats |
| title_fullStr | Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats |
| title_full_unstemmed | Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats |
| title_short | Synbiotic effects of β-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats |
| title_sort | synbiotic effects of β glucans from cauliflower mushroom and lactobacillus fermentum on metabolic changes and gut microbiome in estrogen deficient rats |
| topic | Cauliflower mushroom β-Glucan Lactobacillus fermentum Gut microbiota Ovariectomy Synbiotics |
| url | http://link.springer.com/article/10.1186/s12263-017-0585-z |
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