Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells
<p>Abstract</p> <p>Background</p> <p>We recently isolated and identified Zizimin2 as a functional factor that is highly expressed in murine splenic germinal center B cells after immunization with T-cell-dependent antigen. Zizimin2 was revealed to be a new family member...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-04-01
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| Series: | Immunity & Ageing |
| Subjects: | |
| Online Access: | http://www.immunityageing.com/content/9/1/2 |
| _version_ | 1819086755654008832 |
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| author | Sakabe Isamu Asai Azusa Iijima Junko Maruyama Mitsuo |
| author_facet | Sakabe Isamu Asai Azusa Iijima Junko Maruyama Mitsuo |
| author_sort | Sakabe Isamu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We recently isolated and identified Zizimin2 as a functional factor that is highly expressed in murine splenic germinal center B cells after immunization with T-cell-dependent antigen. Zizimin2 was revealed to be a new family member of Dock (dedicator of cytokinesis), Dock11, which is the guanine nucleotide exchange factor for Cdc42, a low-molecular-weight GTPase. However, the molecular function of Zizimin2 in acquired immunity has not been elucidated.</p> <p>Results</p> <p>In this study, we show that the protein expression of Zizimin2, which is also restricted to lymphoid tissues and lymphocytes, is reduced in aged mice. Over-expression of full-length Zizimin2 induced filopodial formation in 293T cells, whereas expression of CZH2 domain inhibited it. Stimulation of Fcγ receptor and Toll-like receptor 4 triggered Zizimin2 up-regulation and Cdc42 activation in bone marrow-derived dendritic cells.</p> <p>Conclusions</p> <p>These data suggest that Zizimin2 is an immune-related and age-regulated guanine nucleotide exchange factor, which facilitates filopodial formation through activation of Cdc42, which results in activation of cell migration.</p> |
| first_indexed | 2024-12-21T21:25:18Z |
| format | Article |
| id | doaj.art-e719b6ed491e419daafc244cbf5b7996 |
| institution | Directory Open Access Journal |
| issn | 1742-4933 |
| language | English |
| last_indexed | 2024-12-21T21:25:18Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Immunity & Ageing |
| spelling | doaj.art-e719b6ed491e419daafc244cbf5b79962022-12-21T18:49:47ZengBMCImmunity & Ageing1742-49332012-04-0191210.1186/1742-4933-9-2Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cellsSakabe IsamuAsai AzusaIijima JunkoMaruyama Mitsuo<p>Abstract</p> <p>Background</p> <p>We recently isolated and identified Zizimin2 as a functional factor that is highly expressed in murine splenic germinal center B cells after immunization with T-cell-dependent antigen. Zizimin2 was revealed to be a new family member of Dock (dedicator of cytokinesis), Dock11, which is the guanine nucleotide exchange factor for Cdc42, a low-molecular-weight GTPase. However, the molecular function of Zizimin2 in acquired immunity has not been elucidated.</p> <p>Results</p> <p>In this study, we show that the protein expression of Zizimin2, which is also restricted to lymphoid tissues and lymphocytes, is reduced in aged mice. Over-expression of full-length Zizimin2 induced filopodial formation in 293T cells, whereas expression of CZH2 domain inhibited it. Stimulation of Fcγ receptor and Toll-like receptor 4 triggered Zizimin2 up-regulation and Cdc42 activation in bone marrow-derived dendritic cells.</p> <p>Conclusions</p> <p>These data suggest that Zizimin2 is an immune-related and age-regulated guanine nucleotide exchange factor, which facilitates filopodial formation through activation of Cdc42, which results in activation of cell migration.</p>http://www.immunityageing.com/content/9/1/2GEFZizimin2FilopodiaCdc42⋅ Dendritic cells |
| spellingShingle | Sakabe Isamu Asai Azusa Iijima Junko Maruyama Mitsuo Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells Immunity & Ageing GEF Zizimin2 Filopodia Cdc42⋅ Dendritic cells |
| title | Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells |
| title_full | Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells |
| title_fullStr | Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells |
| title_full_unstemmed | Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells |
| title_short | Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells |
| title_sort | age related guanine nucleotide exchange factor mouse zizimin2 induces filopodia in bone marrow derived dendritic cells |
| topic | GEF Zizimin2 Filopodia Cdc42⋅ Dendritic cells |
| url | http://www.immunityageing.com/content/9/1/2 |
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