Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells
The family Bignoniaceae includes <i>Handroanthus impetiginosus</i> trees, which are sparsely distributed in the northeast of Brazil. Natural products play a vital role in the discovery of drugs for various diseases. Many plants have been used as sources of medicines because of their chem...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/24/13171 |
| _version_ | 1827575798434889728 |
|---|---|
| author | Jinnatun Nahar Md Niaj Morshed Esrat Jahan Rupa Jung Hyeok Lee Anjali Kariyarath Valappil Muhammad Awais Ko Jeong Hun Lee Ji Sook Md. Al-Amin Jong Chan Ahn Deok Chun Yang Seok-Kyu Jung |
| author_facet | Jinnatun Nahar Md Niaj Morshed Esrat Jahan Rupa Jung Hyeok Lee Anjali Kariyarath Valappil Muhammad Awais Ko Jeong Hun Lee Ji Sook Md. Al-Amin Jong Chan Ahn Deok Chun Yang Seok-Kyu Jung |
| author_sort | Jinnatun Nahar |
| collection | DOAJ |
| description | The family Bignoniaceae includes <i>Handroanthus impetiginosus</i> trees, which are sparsely distributed in the northeast of Brazil. Natural products play a vital role in the discovery of drugs for various diseases. Many plants have been used as sources of medicines because of their chemical diversity and potent bioactivity. <i>Handroanthus impetiginosus</i> has been used traditionally to cure a wide range of illnesses, such as cancer, oxidative stress, and inflammation. This work highlights the cytotoxicity, cell death, and routes of apoptosis in lung cancer cells (A549) and the anti-inflammatory and antioxidant effects of roasted <i>Handroanthus impetiginosus</i> (lapacho/taheebo) in normal cells. The cell viability assay indicated that puffing roasted taheebo is nontoxic to a normal cell line up to 500 µg/mL but significantly toxic to A549 cells. The roasted lapacho/taheebo also increases reactive oxygen species (ROS) generation in A549 lung cancer cells, and cellular apoptosis via a mitochondrial intrinsic pathway was confirmed. The roasted lapacho/taheebo significantly inhibited both colony formation and cell migration ability, highlighting its potential as an anticancer agent. Additionally, this study demonstrates that roasted taheebo enhanced the expression of genes for BAX accumulation and decreased Bcl-2 gene expression through the p53 signaling pathway. Furthermore, research on the anti-inflammatory properties of roasted taheebo revealed a strong NO inhibition as well as the inhibition of inflammatory mediators (TNF-α, iNOS, COX-2, IL-6, and IL-8) through the NF-κB signaling pathway. However, in H<sub>2</sub>O<sub>2</sub>-induced HaCaT cells, roasted taheebo extract significantly reduced oxidative stress by upregulating the level of expression of antioxidative markers (SOD, CAT, GPx, and GST) at 50 μg/mL. As a result, roasted taheebo justifies investigation in animal and clinical trials as a possible source of antioxidants, anti-inflammatory substances, and anti-cancer compounds. |
| first_indexed | 2024-03-08T21:02:06Z |
| format | Article |
| id | doaj.art-e71d09b7836e49cdaf22a664264fca69 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-08T21:02:06Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-e71d09b7836e49cdaf22a664264fca692023-12-22T13:51:43ZengMDPI AGApplied Sciences2076-34172023-12-0113241317110.3390/app132413171Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal CellsJinnatun Nahar0Md Niaj Morshed1Esrat Jahan Rupa2Jung Hyeok Lee3Anjali Kariyarath Valappil4Muhammad Awais5Ko Jeong Hun6Lee Ji Sook7Md. Al-Amin8Jong Chan Ahn9Deok Chun Yang10Seok-Kyu Jung11Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaDepartment of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaDepartment of Biopharmaceutical Biotechnology, College of Life Science, Kyung Hee University, Yongin-si 17104, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaNK TAHEEBO Company, 27, Dongtancheomdansaneop 1-ro, Hwaseong-si 18445, Republic of KoreaNK TAHEEBO Company, 27, Dongtancheomdansaneop 1-ro, Hwaseong-si 18445, Republic of KoreaDepartment of Chemistry Education, Graduate Department of Chemical Materials, Pusan National University, Busan 46241, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Republic of KoreaDepartment of Horticulture, Kongju National University, Yesan 32439, Republic of KoreaThe family Bignoniaceae includes <i>Handroanthus impetiginosus</i> trees, which are sparsely distributed in the northeast of Brazil. Natural products play a vital role in the discovery of drugs for various diseases. Many plants have been used as sources of medicines because of their chemical diversity and potent bioactivity. <i>Handroanthus impetiginosus</i> has been used traditionally to cure a wide range of illnesses, such as cancer, oxidative stress, and inflammation. This work highlights the cytotoxicity, cell death, and routes of apoptosis in lung cancer cells (A549) and the anti-inflammatory and antioxidant effects of roasted <i>Handroanthus impetiginosus</i> (lapacho/taheebo) in normal cells. The cell viability assay indicated that puffing roasted taheebo is nontoxic to a normal cell line up to 500 µg/mL but significantly toxic to A549 cells. The roasted lapacho/taheebo also increases reactive oxygen species (ROS) generation in A549 lung cancer cells, and cellular apoptosis via a mitochondrial intrinsic pathway was confirmed. The roasted lapacho/taheebo significantly inhibited both colony formation and cell migration ability, highlighting its potential as an anticancer agent. Additionally, this study demonstrates that roasted taheebo enhanced the expression of genes for BAX accumulation and decreased Bcl-2 gene expression through the p53 signaling pathway. Furthermore, research on the anti-inflammatory properties of roasted taheebo revealed a strong NO inhibition as well as the inhibition of inflammatory mediators (TNF-α, iNOS, COX-2, IL-6, and IL-8) through the NF-κB signaling pathway. However, in H<sub>2</sub>O<sub>2</sub>-induced HaCaT cells, roasted taheebo extract significantly reduced oxidative stress by upregulating the level of expression of antioxidative markers (SOD, CAT, GPx, and GST) at 50 μg/mL. As a result, roasted taheebo justifies investigation in animal and clinical trials as a possible source of antioxidants, anti-inflammatory substances, and anti-cancer compounds.https://www.mdpi.com/2076-3417/13/24/13171<i>Handroanthus impetiginosus</i>ROS generationanti-lung canceranti-inflammationantioxidant |
| spellingShingle | Jinnatun Nahar Md Niaj Morshed Esrat Jahan Rupa Jung Hyeok Lee Anjali Kariyarath Valappil Muhammad Awais Ko Jeong Hun Lee Ji Sook Md. Al-Amin Jong Chan Ahn Deok Chun Yang Seok-Kyu Jung Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells Applied Sciences <i>Handroanthus impetiginosus</i> ROS generation anti-lung cancer anti-inflammation antioxidant |
| title | Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells |
| title_full | Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells |
| title_fullStr | Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells |
| title_full_unstemmed | Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells |
| title_short | Roasting Extract of <i>Handroanthus impetiginosus</i> Enhances Its Anticancer Activity in A549 Lung Cancer Cells and Improves Its Antioxidant and Anti-Inflammatory Effects in Normal Cells |
| title_sort | roasting extract of i handroanthus impetiginosus i enhances its anticancer activity in a549 lung cancer cells and improves its antioxidant and anti inflammatory effects in normal cells |
| topic | <i>Handroanthus impetiginosus</i> ROS generation anti-lung cancer anti-inflammation antioxidant |
| url | https://www.mdpi.com/2076-3417/13/24/13171 |
| work_keys_str_mv | AT jinnatunnahar roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT mdniajmorshed roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT esratjahanrupa roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT junghyeoklee roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT anjalikariyarathvalappil roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT muhammadawais roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT kojeonghun roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT leejisook roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT mdalamin roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT jongchanahn roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT deokchunyang roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells AT seokkyujung roastingextractofihandroanthusimpetiginosusienhancesitsanticanceractivityina549lungcancercellsandimprovesitsantioxidantandantiinflammatoryeffectsinnormalcells |