Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site.
TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4234655?pdf=render |
| _version_ | 1818034608494608384 |
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| author | Rossana Cocchiola Caterina Grillo Fabio Altieri Silvia Chichiarelli Carlo Turano Margherita Eufemi |
| author_facet | Rossana Cocchiola Caterina Grillo Fabio Altieri Silvia Chichiarelli Carlo Turano Margherita Eufemi |
| author_sort | Rossana Cocchiola |
| collection | DOAJ |
| description | TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site. |
| first_indexed | 2024-12-10T06:41:52Z |
| format | Article |
| id | doaj.art-e71f5443c102412d85f5839da53fcc54 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T06:41:52Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e71f5443c102412d85f5839da53fcc542022-12-22T01:58:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11309610.1371/journal.pone.0113096Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site.Rossana CocchiolaCaterina GrilloFabio AltieriSilvia ChichiarelliCarlo TuranoMargherita EufemiTPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site.http://europepmc.org/articles/PMC4234655?pdf=render |
| spellingShingle | Rossana Cocchiola Caterina Grillo Fabio Altieri Silvia Chichiarelli Carlo Turano Margherita Eufemi Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. PLoS ONE |
| title | Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. |
| title_full | Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. |
| title_fullStr | Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. |
| title_full_unstemmed | Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. |
| title_short | Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site. |
| title_sort | upregulation of tpx2 by stat3 identification of a novel stat3 binding site |
| url | http://europepmc.org/articles/PMC4234655?pdf=render |
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