Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal
The purpose of this study was to analyse the prevalence and genetic characteristics of ESBL and acquired-AmpC (qAmpC)-producing <i>Escherichia coli</i> isolates from healthy and sick dogs in Portugal. Three hundred and sixty-one faecal samples from sick and healthy dogs were seeded on Ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/8/1013 |
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| author | Isabel Carvalho Rita Cunha Carla Martins Sandra Martínez-Álvarez Nadia Safia Chenouf Paulo Pimenta Ana Raquel Pereira Sónia Ramos Madjid Sadi Ângela Martins Jorge Façanha Fazle Rabbi Rosa Capita Carlos Alonso-Calleja Maria de Lurdes Nunes Enes Dapkevicius Gilberto Igrejas Carmen Torres Patrícia Poeta |
| author_facet | Isabel Carvalho Rita Cunha Carla Martins Sandra Martínez-Álvarez Nadia Safia Chenouf Paulo Pimenta Ana Raquel Pereira Sónia Ramos Madjid Sadi Ângela Martins Jorge Façanha Fazle Rabbi Rosa Capita Carlos Alonso-Calleja Maria de Lurdes Nunes Enes Dapkevicius Gilberto Igrejas Carmen Torres Patrícia Poeta |
| author_sort | Isabel Carvalho |
| collection | DOAJ |
| description | The purpose of this study was to analyse the prevalence and genetic characteristics of ESBL and acquired-AmpC (qAmpC)-producing <i>Escherichia coli</i> isolates from healthy and sick dogs in Portugal. Three hundred and sixty-one faecal samples from sick and healthy dogs were seeded on MacConkey agar supplemented with cefotaxime (2 µg/mL) for cefotaxime-resistant (CTX<sup>R</sup>) <i>E. coli</i> recovery. Antimicrobial susceptibility testing for 15 antibiotics was performed and the ESBL-phenotype of the <i>E. coli</i> isolates was screened. Detection of antimicrobial resistance and virulence genes, and molecular typing of the isolates (phylogroups, multilocus-sequence-typing, and specific-ST131) were performed by PCR (and sequencing when required). CTX<sup>R</sup><i>E. coli</i> isolates were obtained in 51/361 faecal samples analysed (14.1%), originating from 36/234 sick dogs and 15/127 healthy dogs. Forty-seven ESBL-producing <i>E. coli</i> isolates were recovered from 32 sick (13.7%) and 15 healthy animals (11.8%). Different variants of <i>bla</i><sub>CTX-M</sub> genes were detected among 45/47 ESBL-producers: <i>bla</i><sub>CTX-M-15</sub> (<i>n</i> = 26), <i>bla</i><sub>CTX-M-1</sub> (<i>n</i> = 10), <i>bla</i><sub>CTX-M-32</sub> (<i>n</i> = 3), <i>bla</i><sub>CTX-M-55</sub> (<i>n</i> = 3), <i>bla</i><sub>CTX-M-14</sub> (<i>n</i> = 2), and <i>bla</i><sub>CTX-M</sub>-variant (<i>n</i> = 1); one ESBL-positive isolate co-produced CTX-M-15 and CMY-2 enzymes. Moreover, two additional CTX<sup>R</sup> ESBL-negative <i>E. coli</i> isolates were CMY-2-producers (qAmpC). Ten different sequence types were identified (ST/phylogenetic-group/β-lactamase): ST131/B2/CTX-M-15, ST617/A/CTX-M-55, ST3078/B1/CTX-M-32, ST542/A/CTX-M-14, ST57/D/CTX-M-1, ST12/B2/CTX-M-15, ST6448/B1/CTX-M-15 + CMY-2, ST5766/A/CTX-M-32, ST115/D/CMY-2 and a new-ST/D/CMY-2. Five variants of CTX-M enzymes (CTX-M-15 and CTX-M-1 predominant) and eight different clonal complexes were detected from canine ESBL-producing <i>E. coli</i> isolates. Although at a lower rate, CMY-2 β-lactamase was also found. Dogs remain frequent carriers of ESBL and/or qAmpC-producing <i>E. coli</i> with a potential zoonotic role. |
| first_indexed | 2024-03-10T09:03:48Z |
| format | Article |
| id | doaj.art-e720693c713a4a8ebbdcdf0f369cb7ad |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:03:48Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-e720693c713a4a8ebbdcdf0f369cb7ad2023-11-22T06:34:41ZengMDPI AGAntibiotics2079-63822021-08-01108101310.3390/antibiotics10081013Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in PortugalIsabel Carvalho0Rita Cunha1Carla Martins2Sandra Martínez-Álvarez3Nadia Safia Chenouf4Paulo Pimenta5Ana Raquel Pereira6Sónia Ramos7Madjid Sadi8Ângela Martins9Jorge Façanha10Fazle Rabbi11Rosa Capita12Carlos Alonso-Calleja13Maria de Lurdes Nunes Enes Dapkevicius14Gilberto Igrejas15Carmen Torres16Patrícia Poeta17Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalHospital Veterinário Cascais da Onevet, 2775-352 Parede, Lisbon, PortugalClínica Veterinária do Vouga, 3740-253 Sever do Vouga, PortugalArea Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, SpainArea Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, SpainHospital Veterinário de Trás-os-Montes, 5000-056 Vila Real, PortugalCentro Veterinário de Macedo de Cavaleiros, 5340-202 Bragança, PortugalVetRedondo, Consultório Veterinário de Monte Redondo Unipessoal Lda, Monte Redondo, 2425-618 Leiria, PortugalArea Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, SpainAnimal and Veterinary Research Center (CECAV), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalCentro Veterinário Jorge Façanha, 5140-060 Carrazeda de Ansiães, PortugalAustralian Computer Society, Docklands, Melbourne, VIC 3008, AustraliaDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, 24071 León, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, 24071 León, SpainFaculty of Agricultural and Environmental Sciences, University of the Azores, 9500-321 Angra do Heroísmo, PortugalDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalArea Biochemistry and Molecular Biology, University of La Rioja, 26006 Logroño, SpainMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalThe purpose of this study was to analyse the prevalence and genetic characteristics of ESBL and acquired-AmpC (qAmpC)-producing <i>Escherichia coli</i> isolates from healthy and sick dogs in Portugal. Three hundred and sixty-one faecal samples from sick and healthy dogs were seeded on MacConkey agar supplemented with cefotaxime (2 µg/mL) for cefotaxime-resistant (CTX<sup>R</sup>) <i>E. coli</i> recovery. Antimicrobial susceptibility testing for 15 antibiotics was performed and the ESBL-phenotype of the <i>E. coli</i> isolates was screened. Detection of antimicrobial resistance and virulence genes, and molecular typing of the isolates (phylogroups, multilocus-sequence-typing, and specific-ST131) were performed by PCR (and sequencing when required). CTX<sup>R</sup><i>E. coli</i> isolates were obtained in 51/361 faecal samples analysed (14.1%), originating from 36/234 sick dogs and 15/127 healthy dogs. Forty-seven ESBL-producing <i>E. coli</i> isolates were recovered from 32 sick (13.7%) and 15 healthy animals (11.8%). Different variants of <i>bla</i><sub>CTX-M</sub> genes were detected among 45/47 ESBL-producers: <i>bla</i><sub>CTX-M-15</sub> (<i>n</i> = 26), <i>bla</i><sub>CTX-M-1</sub> (<i>n</i> = 10), <i>bla</i><sub>CTX-M-32</sub> (<i>n</i> = 3), <i>bla</i><sub>CTX-M-55</sub> (<i>n</i> = 3), <i>bla</i><sub>CTX-M-14</sub> (<i>n</i> = 2), and <i>bla</i><sub>CTX-M</sub>-variant (<i>n</i> = 1); one ESBL-positive isolate co-produced CTX-M-15 and CMY-2 enzymes. Moreover, two additional CTX<sup>R</sup> ESBL-negative <i>E. coli</i> isolates were CMY-2-producers (qAmpC). Ten different sequence types were identified (ST/phylogenetic-group/β-lactamase): ST131/B2/CTX-M-15, ST617/A/CTX-M-55, ST3078/B1/CTX-M-32, ST542/A/CTX-M-14, ST57/D/CTX-M-1, ST12/B2/CTX-M-15, ST6448/B1/CTX-M-15 + CMY-2, ST5766/A/CTX-M-32, ST115/D/CMY-2 and a new-ST/D/CMY-2. Five variants of CTX-M enzymes (CTX-M-15 and CTX-M-1 predominant) and eight different clonal complexes were detected from canine ESBL-producing <i>E. coli</i> isolates. Although at a lower rate, CMY-2 β-lactamase was also found. Dogs remain frequent carriers of ESBL and/or qAmpC-producing <i>E. coli</i> with a potential zoonotic role.https://www.mdpi.com/2079-6382/10/8/1013antimicrobial resistancedogs<i>Escherichia coli</i>ESBLCTX-M-15CTX-M-1 |
| spellingShingle | Isabel Carvalho Rita Cunha Carla Martins Sandra Martínez-Álvarez Nadia Safia Chenouf Paulo Pimenta Ana Raquel Pereira Sónia Ramos Madjid Sadi Ângela Martins Jorge Façanha Fazle Rabbi Rosa Capita Carlos Alonso-Calleja Maria de Lurdes Nunes Enes Dapkevicius Gilberto Igrejas Carmen Torres Patrícia Poeta Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal Antibiotics antimicrobial resistance dogs <i>Escherichia coli</i> ESBL CTX-M-15 CTX-M-1 |
| title | Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal |
| title_full | Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal |
| title_fullStr | Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal |
| title_full_unstemmed | Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal |
| title_short | Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing <i>Escherichia coli</i> Isolated from Healthy and Sick Dogs Living in Portugal |
| title_sort | antimicrobial resistance genes and diversity of clones among faecal esbl producing i escherichia coli i isolated from healthy and sick dogs living in portugal |
| topic | antimicrobial resistance dogs <i>Escherichia coli</i> ESBL CTX-M-15 CTX-M-1 |
| url | https://www.mdpi.com/2079-6382/10/8/1013 |
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