Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound
Abstract Recently, ultrasound (US)-based drug delivery strategies have received attention to improve enhanced permeation and retention (EPR) effect-based passive targeting efficiency of nanoparticles in vitro and in vivo conditions. Among the US treatment techniques, pulsed-high intensity focused ul...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-11-01
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| Series: | Nano Convergence |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40580-017-0124-z |
| _version_ | 1818109833412345856 |
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| author | Dong Gil You Hong Yeol Yoon Sangmin Jeon Wooram Um Sejin Son Jae Hyung Park Ick Chan Kwon Kwangmeyung Kim |
| author_facet | Dong Gil You Hong Yeol Yoon Sangmin Jeon Wooram Um Sejin Son Jae Hyung Park Ick Chan Kwon Kwangmeyung Kim |
| author_sort | Dong Gil You |
| collection | DOAJ |
| description | Abstract Recently, ultrasound (US)-based drug delivery strategies have received attention to improve enhanced permeation and retention (EPR) effect-based passive targeting efficiency of nanoparticles in vitro and in vivo conditions. Among the US treatment techniques, pulsed-high intensity focused ultrasound (pHIFU) have specialized for improving tissue penetration of various macromolecules and nanoparticles without irreversible tissue damages. In this study, we have demonstrated that pHIFU could be utilized to improve tissue penetration of fluorescent dye-labeled glycol chitosan nanoparticles (FCNPs) in femoral tissue of mice. pHIFU could improve blood flow of the targeted-blood vessel in femoral tissue. In addition, tissue penetration of FCNPs was specifically increased 5.7-, 8- and 9.3-folds than that of non-treated (0 W pHIFU) femoral tissue, when the femoral tissue was treated with 10, 20 and 50 W of pHIFU, respectively. However, tissue penetration of FCNPs was significantly reduced after 3 h post-pHIFU treatment (50 W). Because overdose (50 W) of pHIFU led to irreversible tissue damages, including the edema and chapped red blood cells. These overall results support that pHIFU treatment can enhance the extravasation and tissue penetration of FCNPs as well as induce irreversible tissue damages. We expect that our results can provide advantages to optimize pHIFU-mediated delivery strategy of nanoparticles for further clinical applications. |
| first_indexed | 2024-12-11T02:37:32Z |
| format | Article |
| id | doaj.art-e7237072f0384f49b22555f9648a2a87 |
| institution | Directory Open Access Journal |
| issn | 2196-5404 |
| language | English |
| last_indexed | 2024-12-11T02:37:32Z |
| publishDate | 2017-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano Convergence |
| spelling | doaj.art-e7237072f0384f49b22555f9648a2a872022-12-22T01:23:42ZengSpringerOpenNano Convergence2196-54042017-11-014111010.1186/s40580-017-0124-zDeep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasoundDong Gil You0Hong Yeol Yoon1Sangmin Jeon2Wooram Um3Sejin Son4Jae Hyung Park5Ick Chan Kwon6Kwangmeyung Kim7School of Chemical Engineering, Sungkyunkwan UniversityCenter for Theragnosis, Biomedical Research Institute, Korea Institute of Science and TechnologySchool of Chemical Engineering, Sungkyunkwan UniversitySchool of Chemical Engineering, Sungkyunkwan UniversityCenter for Theragnosis, Biomedical Research Institute, Korea Institute of Science and TechnologySchool of Chemical Engineering, Sungkyunkwan UniversityCenter for Theragnosis, Biomedical Research Institute, Korea Institute of Science and TechnologyCenter for Theragnosis, Biomedical Research Institute, Korea Institute of Science and TechnologyAbstract Recently, ultrasound (US)-based drug delivery strategies have received attention to improve enhanced permeation and retention (EPR) effect-based passive targeting efficiency of nanoparticles in vitro and in vivo conditions. Among the US treatment techniques, pulsed-high intensity focused ultrasound (pHIFU) have specialized for improving tissue penetration of various macromolecules and nanoparticles without irreversible tissue damages. In this study, we have demonstrated that pHIFU could be utilized to improve tissue penetration of fluorescent dye-labeled glycol chitosan nanoparticles (FCNPs) in femoral tissue of mice. pHIFU could improve blood flow of the targeted-blood vessel in femoral tissue. In addition, tissue penetration of FCNPs was specifically increased 5.7-, 8- and 9.3-folds than that of non-treated (0 W pHIFU) femoral tissue, when the femoral tissue was treated with 10, 20 and 50 W of pHIFU, respectively. However, tissue penetration of FCNPs was significantly reduced after 3 h post-pHIFU treatment (50 W). Because overdose (50 W) of pHIFU led to irreversible tissue damages, including the edema and chapped red blood cells. These overall results support that pHIFU treatment can enhance the extravasation and tissue penetration of FCNPs as well as induce irreversible tissue damages. We expect that our results can provide advantages to optimize pHIFU-mediated delivery strategy of nanoparticles for further clinical applications.http://link.springer.com/article/10.1186/s40580-017-0124-zPulsed-high intensity focused ultrasoundTissue penetrationNanoparticleDrug delivery |
| spellingShingle | Dong Gil You Hong Yeol Yoon Sangmin Jeon Wooram Um Sejin Son Jae Hyung Park Ick Chan Kwon Kwangmeyung Kim Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound Nano Convergence Pulsed-high intensity focused ultrasound Tissue penetration Nanoparticle Drug delivery |
| title | Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound |
| title_full | Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound |
| title_fullStr | Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound |
| title_full_unstemmed | Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound |
| title_short | Deep tissue penetration of nanoparticles using pulsed-high intensity focused ultrasound |
| title_sort | deep tissue penetration of nanoparticles using pulsed high intensity focused ultrasound |
| topic | Pulsed-high intensity focused ultrasound Tissue penetration Nanoparticle Drug delivery |
| url | http://link.springer.com/article/10.1186/s40580-017-0124-z |
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