LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer
Gastric cancer (GC) is among the most lethal tumors worldwide. Long noncoding RNAs (lncRNAs) are reported to be critical during the occurrence and progression of malignancies. The HOXC cluster antisense RNA 1 (HOXC-AS1) has been suggested to participate in the genesis and development of GC. Therefor...
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MDPI AG
2022-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/14/3534 |
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| author | Yue Jiang Xiangpan Li Yu Yang Jiajun Luo Xunshan Ren Jingwen Yuan Qiang Tong |
| author_facet | Yue Jiang Xiangpan Li Yu Yang Jiajun Luo Xunshan Ren Jingwen Yuan Qiang Tong |
| author_sort | Yue Jiang |
| collection | DOAJ |
| description | Gastric cancer (GC) is among the most lethal tumors worldwide. Long noncoding RNAs (lncRNAs) are reported to be critical during the occurrence and progression of malignancies. The HOXC cluster antisense RNA 1 (HOXC-AS1) has been suggested to participate in the genesis and development of GC. Therefore, we examined GC cells and tissues for the expression of HOXC-AS1 and correlated the expression levels with the disease specific survival of the patients, finding that HOXC-AS1 was overexpressed and probably had a tendency of leading to a poor prognosis. The Cell Counting Kit-8 assay and colony formation assay were then performed under knockdown of HOXC-AS1, revealing that cell proliferation of GC was distinctly decreased. Afterwards, miR-99a-3p was predicted to bind with HOXC-AS1 by DIANA tools. We carried out dual-luciferase reporter gene assays to identify the interaction between them. After knockdown of HOXC-AS1, miR-99a-3p was clearly overexpressed in GC cells. In addition, matrix metalloproteinase 8 (MMP8) was shown to be combined with miR-99a-3p using TargetScan. Similar experiments, along with western blot, were conducted to validate the correlation between miR-99a-3p and MMP8. Finally, rescue experiments for CCK-8 were completed, disclosing that HOXC-AS1 promoted cell progression of GC through sponging miR-99a-3p followed by subsequent upregulation of MMP8. |
| first_indexed | 2024-03-09T03:36:57Z |
| format | Article |
| id | doaj.art-e723e03c02b0496982fb8e83dc57cad6 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T03:36:57Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e723e03c02b0496982fb8e83dc57cad62023-12-03T14:48:15ZengMDPI AGCancers2072-66942022-07-011414353410.3390/cancers14143534LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric CancerYue Jiang0Xiangpan Li1Yu Yang2Jiajun Luo3Xunshan Ren4Jingwen Yuan5Qiang Tong6Department of Gastrointestinal Surgery I Section, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Gastrointestinal Surgery I Section, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Gastrointestinal Surgery I Section, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Gastrointestinal Surgery I Section, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Gastrointestinal Surgery I Section, Renmin Hospital of Wuhan University, Wuhan 430060, ChinaGastric cancer (GC) is among the most lethal tumors worldwide. Long noncoding RNAs (lncRNAs) are reported to be critical during the occurrence and progression of malignancies. The HOXC cluster antisense RNA 1 (HOXC-AS1) has been suggested to participate in the genesis and development of GC. Therefore, we examined GC cells and tissues for the expression of HOXC-AS1 and correlated the expression levels with the disease specific survival of the patients, finding that HOXC-AS1 was overexpressed and probably had a tendency of leading to a poor prognosis. The Cell Counting Kit-8 assay and colony formation assay were then performed under knockdown of HOXC-AS1, revealing that cell proliferation of GC was distinctly decreased. Afterwards, miR-99a-3p was predicted to bind with HOXC-AS1 by DIANA tools. We carried out dual-luciferase reporter gene assays to identify the interaction between them. After knockdown of HOXC-AS1, miR-99a-3p was clearly overexpressed in GC cells. In addition, matrix metalloproteinase 8 (MMP8) was shown to be combined with miR-99a-3p using TargetScan. Similar experiments, along with western blot, were conducted to validate the correlation between miR-99a-3p and MMP8. Finally, rescue experiments for CCK-8 were completed, disclosing that HOXC-AS1 promoted cell progression of GC through sponging miR-99a-3p followed by subsequent upregulation of MMP8.https://www.mdpi.com/2072-6694/14/14/3534gastric cancerHOXC-AS1miR-99a-3pmatrix metallopeptidase 8ceRNA network |
| spellingShingle | Yue Jiang Xiangpan Li Yu Yang Jiajun Luo Xunshan Ren Jingwen Yuan Qiang Tong LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer Cancers gastric cancer HOXC-AS1 miR-99a-3p matrix metallopeptidase 8 ceRNA network |
| title | LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer |
| title_full | LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer |
| title_fullStr | LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer |
| title_full_unstemmed | LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer |
| title_short | LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer |
| title_sort | lncrna hoxc as1 sponges mir 99a 3p and upregulates mmp8 ultimately promoting gastric cancer |
| topic | gastric cancer HOXC-AS1 miR-99a-3p matrix metallopeptidase 8 ceRNA network |
| url | https://www.mdpi.com/2072-6694/14/14/3534 |
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