Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress
The NAD+-dependent deacetylases Sirt1 and Sirt2 mediate cellular stress responses and are highly expressed in vascular endothelial cells. In contrast to the well-documented protective actions of Sirt1, the role of endothelial Sirt2 remains unknown. Using cDNA microarray and PCR validation, we examin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/14/3/5633 |
| _version_ | 1811277656750030848 |
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| author | Qunye Zhang Fan Jiang Peili Bu Yun Zhang Xi Wang Xiao Wu Junni Liu |
| author_facet | Qunye Zhang Fan Jiang Peili Bu Yun Zhang Xi Wang Xiao Wu Junni Liu |
| author_sort | Qunye Zhang |
| collection | DOAJ |
| description | The NAD+-dependent deacetylases Sirt1 and Sirt2 mediate cellular stress responses and are highly expressed in vascular endothelial cells. In contrast to the well-documented protective actions of Sirt1, the role of endothelial Sirt2 remains unknown. Using cDNA microarray and PCR validation, we examined global gene expression changes in response to Sirt2 knock down in primary human umbilical vein endothelial cells under oxidative stress. We found that Sirt2 knock down changed expression of 340 genes, which are mainly involved in cellular processes including actin binding, cellular amino acid metabolic process, transmembrane receptor protein serine/threonine kinase signaling, ferrous iron transport, protein transport and localization, cell morphogenesis, and functions associated with endosome membrane and the trans-Golgi network. These genes and associated functions were largely non-overlapping with those altered by Sirt1 knock down. Moreover, we showed that pharmacological inhibition of Sirt2 attenuated oxidant-induced cell toxicity in endothelial cells. These suggest that Sirt2 is functionally important in endothelial cells under oxidative stress, and may have a primarily distinct role as compared to Sirt1. Our results may provide a basis for future studies aiming to dissect the specific signaling pathway(s) that mediates specific Sirt2 functions in endothelial cells. |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T00:19:35Z |
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| spelling | doaj.art-e724d0cf917243ffbd38142def37adc22022-12-22T03:10:49ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-03-011435633564910.3390/ijms14035633Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative StressQunye ZhangFan JiangPeili BuYun ZhangXi WangXiao WuJunni LiuThe NAD+-dependent deacetylases Sirt1 and Sirt2 mediate cellular stress responses and are highly expressed in vascular endothelial cells. In contrast to the well-documented protective actions of Sirt1, the role of endothelial Sirt2 remains unknown. Using cDNA microarray and PCR validation, we examined global gene expression changes in response to Sirt2 knock down in primary human umbilical vein endothelial cells under oxidative stress. We found that Sirt2 knock down changed expression of 340 genes, which are mainly involved in cellular processes including actin binding, cellular amino acid metabolic process, transmembrane receptor protein serine/threonine kinase signaling, ferrous iron transport, protein transport and localization, cell morphogenesis, and functions associated with endosome membrane and the trans-Golgi network. These genes and associated functions were largely non-overlapping with those altered by Sirt1 knock down. Moreover, we showed that pharmacological inhibition of Sirt2 attenuated oxidant-induced cell toxicity in endothelial cells. These suggest that Sirt2 is functionally important in endothelial cells under oxidative stress, and may have a primarily distinct role as compared to Sirt1. Our results may provide a basis for future studies aiming to dissect the specific signaling pathway(s) that mediates specific Sirt2 functions in endothelial cells.http://www.mdpi.com/1422-0067/14/3/5633Sirt1Sirt2endothelial celloxidative stressfunctional genomicsmicroarray |
| spellingShingle | Qunye Zhang Fan Jiang Peili Bu Yun Zhang Xi Wang Xiao Wu Junni Liu Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress International Journal of Molecular Sciences Sirt1 Sirt2 endothelial cell oxidative stress functional genomics microarray |
| title | Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress |
| title_full | Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress |
| title_fullStr | Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress |
| title_full_unstemmed | Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress |
| title_short | Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress |
| title_sort | global gene expression profiling reveals functional importance of sirt2 in endothelial cells under oxidative stress |
| topic | Sirt1 Sirt2 endothelial cell oxidative stress functional genomics microarray |
| url | http://www.mdpi.com/1422-0067/14/3/5633 |
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