Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics
A high performance liquid chromatography-diode array detector (HPLC-DAD) was used to establish the HPLC fingerprint. Chemometrics methods were used to discriminate against the gender of flower buds of Populus tomentosa based on areas of common peaks calibrated with the HPLC fingerprint. The score pl...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/1281521 |
| _version_ | 1797996731665743872 |
|---|---|
| author | Zhuojun Li Cui Wu Bo Xu Huijun Wang Pingping Song Zhenying Liu Zhimao Chao |
| author_facet | Zhuojun Li Cui Wu Bo Xu Huijun Wang Pingping Song Zhenying Liu Zhimao Chao |
| author_sort | Zhuojun Li |
| collection | DOAJ |
| description | A high performance liquid chromatography-diode array detector (HPLC-DAD) was used to establish the HPLC fingerprint. Chemometrics methods were used to discriminate against the gender of flower buds of Populus tomentosa based on areas of common peaks calibrated with the HPLC fingerprint. The score plot of principal component analysis (PCA) showed a clear grouping trend (R2X, 0.753; Q2, 0.564) between female and male samples. Two groups were also well discriminated with orthogonal partial least squares-discriminant analysis (OPLS-DA) (R2X, 0.741; R2Y, 0.980; Q2, 0.970). As the hierarchical clustering analysis (HCA) heatmap showed, all samples were separated into two groups. Four compounds were screened out by S-plot and variable importance in projection (VIP > 1.0). Two of them were identified as siebolside B and tremulacin. This study demonstrated that HPLC fingerprints combined with chemometrics can be applied to discriminate against dioecious plants and screen differences, providing a reference for identifying the gender of dioecious plants. |
| first_indexed | 2024-04-11T10:22:08Z |
| format | Article |
| id | doaj.art-e725a86c4ac140f2ba5bcafec15694cc |
| institution | Directory Open Access Journal |
| issn | 1687-8779 |
| language | English |
| last_indexed | 2024-04-11T10:22:08Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj.art-e725a86c4ac140f2ba5bcafec15694cc2022-12-22T04:29:44ZengHindawi LimitedInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/1281521Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with ChemometricsZhuojun Li0Cui Wu1Bo Xu2Huijun Wang3Pingping Song4Zhenying Liu5Zhimao Chao6Institute of Chinese Materia MedicaInstitute of Chinese Materia MedicaInstitute of Chinese Materia MedicaInstitute of Chinese Materia MedicaInstitute of Chinese Materia MedicaInstitute of Chinese Materia MedicaInstitute of Chinese Materia MedicaA high performance liquid chromatography-diode array detector (HPLC-DAD) was used to establish the HPLC fingerprint. Chemometrics methods were used to discriminate against the gender of flower buds of Populus tomentosa based on areas of common peaks calibrated with the HPLC fingerprint. The score plot of principal component analysis (PCA) showed a clear grouping trend (R2X, 0.753; Q2, 0.564) between female and male samples. Two groups were also well discriminated with orthogonal partial least squares-discriminant analysis (OPLS-DA) (R2X, 0.741; R2Y, 0.980; Q2, 0.970). As the hierarchical clustering analysis (HCA) heatmap showed, all samples were separated into two groups. Four compounds were screened out by S-plot and variable importance in projection (VIP > 1.0). Two of them were identified as siebolside B and tremulacin. This study demonstrated that HPLC fingerprints combined with chemometrics can be applied to discriminate against dioecious plants and screen differences, providing a reference for identifying the gender of dioecious plants.http://dx.doi.org/10.1155/2022/1281521 |
| spellingShingle | Zhuojun Li Cui Wu Bo Xu Huijun Wang Pingping Song Zhenying Liu Zhimao Chao Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics International Journal of Analytical Chemistry |
| title | Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics |
| title_full | Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics |
| title_fullStr | Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics |
| title_full_unstemmed | Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics |
| title_short | Gender Discrimination of Flower Buds of Mature Populus tomentosa by HPLC Fingerprint Combined with Chemometrics |
| title_sort | gender discrimination of flower buds of mature populus tomentosa by hplc fingerprint combined with chemometrics |
| url | http://dx.doi.org/10.1155/2022/1281521 |
| work_keys_str_mv | AT zhuojunli genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT cuiwu genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT boxu genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT huijunwang genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT pingpingsong genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT zhenyingliu genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics AT zhimaochao genderdiscriminationofflowerbudsofmaturepopulustomentosabyhplcfingerprintcombinedwithchemometrics |