A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.
We here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4337908?pdf=render |
| _version_ | 1819068915961036800 |
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| author | Zhe Cheng Fan Liu Shan Zhu Huimin Tian Liang Wang Yanhai Wang |
| author_facet | Zhe Cheng Fan Liu Shan Zhu Huimin Tian Liang Wang Yanhai Wang |
| author_sort | Zhe Cheng |
| collection | DOAJ |
| description | We here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining assays directly. Using these preparations, in combination with conventional fluorescence staining techniques, we could detect the expression and subcellular localization of a specific protein and identify in situ proliferative or apoptotic cells in the germinal layer of metacestode vesicles. Based on this approach, future molecular and cellular analysis of Echinococcus metacestode vesicles in the in vitro system will be greatly facilitated. |
| first_indexed | 2024-12-21T16:41:44Z |
| format | Article |
| id | doaj.art-e728432c09a2447f851b9b3f48d4d0bf |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T16:41:44Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e728432c09a2447f851b9b3f48d4d0bf2022-12-21T18:57:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011821510.1371/journal.pone.0118215A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis.Zhe ChengFan LiuShan ZhuHuimin TianLiang WangYanhai WangWe here describe a convenient method for preparation, fixation and fluorescence analysis of in vitro cultivated metacestode vesicles from E. multilocularis. Parasite materials could be prepared in one hour, did not need to be sectioned, and were subsequently utilized for further whole-mount staining assays directly. Using these preparations, in combination with conventional fluorescence staining techniques, we could detect the expression and subcellular localization of a specific protein and identify in situ proliferative or apoptotic cells in the germinal layer of metacestode vesicles. Based on this approach, future molecular and cellular analysis of Echinococcus metacestode vesicles in the in vitro system will be greatly facilitated.http://europepmc.org/articles/PMC4337908?pdf=render |
| spellingShingle | Zhe Cheng Fan Liu Shan Zhu Huimin Tian Liang Wang Yanhai Wang A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. PLoS ONE |
| title | A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. |
| title_full | A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. |
| title_fullStr | A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. |
| title_full_unstemmed | A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. |
| title_short | A rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from Echinococcus multilocularis. |
| title_sort | rapid and convenient method for fluorescence analysis of in vitro cultivated metacestode vesicles from echinococcus multilocularis |
| url | http://europepmc.org/articles/PMC4337908?pdf=render |
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